##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI AviII
AvrII BbrPI BlnI BsaAI BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI Eco47III EcoRV EcoT22I FseI
FspI HpaI MamI MluI MroI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SbfI SfiI SfuI SgrAI
SnaBI Sse8387I SspBI SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 452 GACGTC GACGT^C
Acc65I 1499 GGTACC G^GTACC
AflIII 2564 ACGTGT A^C[GA][CT]GT
AhdI 1669 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 181 GGGCCC GGGCC^C
Asp700I 1586 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1499 GGTACC G^GTACC
AspEI 1669 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3446 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3908 TGGCCA TGG^CCA
BamHI 899 GGATCC G^GATCC
BbsI 26 GTCTTC GAAGAC(2/6)
BcgI 338 GCATTAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 672 GGATAC GTATCC(6/5)
BclI 1818 TGATCA T^GATCA
BglI 69 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1684 AGATCT A^GATCT
BpuAI 26 GTCTTC GAAGAC(2/6)
BsmBI 484 GAGACG CGTCTC(1/5)
BspHI 351 TCATGA T^CATGA
BsrBI 241 CCGCTC CCGCTC(-3/-3)
BsrDI 886 CATTGC GCAATG(2/0)
BstAPI 1824 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 431 GGTCACC G^GT[ACGT]ACC
BstPI 431 GGTCACC G^GT[ACGT]ACC
DraI 3513 TTTAAA TTT^AAA
DraIII 533 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1669 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3580 GAAGAG CTCTTC(1/4)
EcoO65I 431 GGTCACC G^GT[ACGT]ACC
EcoRI 929 GAATTC G^AATTC
FbaI 1818 TGATCA T^GATCA
HincII 1881 GTTGAC GT[CT]^[GA]AC
HindII 1881 GTTGAC GT[CT]^[GA]AC
HindIII 2428 AAGCTT A^AGCTT
KpnI 1499 GGTACC GGTAC^C
Ksp632I 3580 GAAGAG CTCTTC(1/4)
KspI 147 CCGCGG CCGC^GG
MluNI 3908 TGGCCA TGG^CCA
MscI 3908 TGGCCA TGG^CCA
NaeI 504 GCCGGC GCC^GGC
NdeI 1536 CATATG CA^TATG
NgoMIV 504 GCCGGC G^CCGGC
PflFI 3446 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 351 TCATGA T^CATGA
SacII 147 CCGCGG CCGC^GG
SapI 3580 GAAGAGC GCTCTTC(1/4)
ScaI 2195 AGTACT AGT^ACT
SmaI 856 CCCGGG CCC^GGG
SphI 252 GCATGC GCATG^C
Tth111I 3446 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3981 TCTAGA T^CTAGA
XmaCI 856 CCCGGG C^CCGGG
XmaI 856 CCCGGG C^CCGGG
XmnI 1586 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2044 GTCTAC GT^[AC][GT]AC
3640 GTAGAC
AflII 1870 CTTAAG C^TTAAG
3028 CTTAAG
BbeI 66 GGCGCC GGCGC^C
1320 GGCGCC
BfrI 1870 CTTAAG C^TTAAG
3028 CTTAAG
BsrFI 14 GCCGGT [GA]^CCGG[CT]
504 GCCGGC
Cfr10I 14 GCCGGT [GA]^CCGG[CT]
504 GCCGGC
EagI 144 CGGCCG C^GGCCG
150 CGGCCG
EclXI 144 CGGCCG C^GGCCG
150 CGGCCG
Eco52I 144 CGGCCG C^GGCCG
150 CGGCCG
HgaI 24 GCGTC GACGC(5/10)
2004 GCGTC
KasI 66 GGCGCC G^GCGCC
1320 GGCGCC
MfeI 1998 CAATTG C^AATTG
3464 CAATTG
MunI 1998 CAATTG C^AATTG
3464 CAATTG
NarI 66 GGCGCC GG^CGCC
1320 GGCGCC
PshAI 425 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
541 GACAACAGTC
SexAI 360 ACCTGGT A^CC[AT]GGT
3661 ACCTGGT
SfoI 66 GGCGCC GGC^GCC
1320 GGCGCC
SpeI 704 ACTAGT A^CTAGT
1841 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII