################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AccI AccIII AclI AflIII AgeI AhdI AocI AscI AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BglI BlnI BsaAI BsaWI BseAI BsoBI BspEI BspLU11I BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CpoI Eam1105I Eco81I EcoO65I EcoRI EcoT14I EcoT22I FseI FspI KasI KspI MfeI MluI MluNI MroI MscI MunI NarI NcoI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI StyI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 3405 GACGTC GACGT^C AflII 3926 CTTAAG C^TTAAG ApaI 1750 GGGCCC GGGCC^C AseI 2592 ATTAAT AT^TAAT AsnI 2592 ATTAAT AT^TAAT BcgI 931 CGAAAGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2057 GTATCC GTATCC(6/5) BclI 1235 TGATCA T^GATCA BfrI 3926 CTTAAG C^TTAAG BglII 262 AGATCT A^GATCT BlpI 1914 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1914 GCTCAGC GC^T[ACGT]AGC BsaBI 387 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 362 GAGACC GGTCTC(1/5) BsiEI 2740 CGGCCG CG[GA][CT]^CG BsiWI 1362 CGTACG C^GTACG BsmBI 3407 CGTCTC CGTCTC(1/5) Bsp1407I 3802 TGTACA T^GTACA BspDI 1447 ATCGAT AT^CGAT BsrBI 2136 GAGCGG CCGCTC(-3/-3) BsrGI 3802 TGTACA T^GTACA BssHII 253 GCGCGC G^CGCGC CelII 1914 GCTCAGC GC^T[ACGT]AGC ClaI 1447 ATCGAT AT^CGAT DraIII 1939 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 3103 CCGTGG C^C[GA][CT]GG EaeI 2740 CGGCCG [CT]^GGCC[GA] EagI 2740 CGGCCG C^GGCCG EclXI 2740 CGGCCG C^GGCCG Eco52I 2740 CGGCCG C^GGCCG FbaI 1235 TGATCA T^GATCA HgaI 28 GCGTC GACGC(5/10) HpaI 1487 GTTAAC GTT^AAC MamI 387 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2222 GCCGGC GCC^GGC NdeI 2052 CATATG CA^TATG NgoMIV 2222 GCCGGC G^CCGGC NheI 2888 GCTAGC G^CTAGC PshBI 2592 ATTAAT AT^TAAT SacI 2989 GAGCTC GAGCT^C SapI 525 GAAGAGC GCTCTTC(1/4) SexAI 1283 ACCTGGT A^CC[AT]GGT SmlI 3926 CTTAAG C^T[CT][GA]AG SspBI 3802 TGTACA T^GTACA StuI 2863 AGGCCT AGG^CCT XbaI 3769 TCTAGA T^CTAGA XcmI 586 CCAAACGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 1814 GGTACC G^GTACC 3682 GGTACC AcyI 27 GGCGTC G[GA]^CG[CT]C 3405 GACGTC Aor51HI 2164 AGCGCT AGC^GCT 3256 AGCGCT Asp700I 260 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1158 GAACCAGTTC Asp718I 1814 GGTACC G^GTACC 3682 GGTACC BanI 1814 GGTACC G^G[CT][GA]CC 3682 GGTACC BanII 1750 GGGCCC G[GA]GC[CT]^C 2989 GAGCTC BpmI 2612 CTCCAG CTGGAG(16/14) 3721 CTCCAG BsaHI 27 GGCGTC G[GA]^CG[CT]C 3405 GACGTC BsgI 2083 CTGCAC GTGCAG(16/14) 2823 GTGCAG BspHI 86 TCATGA T^CATGA 2521 TCATGA BsrDI 2982 CATTGC GCAATG(2/0) 3916 GCAATG BsrFI 43 GCCGGT [GA]^CCGG[CT] 2222 GCCGGC BstAPI 2977 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3086 GCAGAAGCTGC BstXI 2782 CCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3481 CCAGAACACTGG BstYI 262 AGATCT [GA]^GATC[CT] 2669 GGATCT Cfr10I 43 GCCGGT [GA]^CCGG[CT] 2222 GCCGGC DraI 2571 TTTAAA TTT^AAA 4021 TTTAAA DrdI 897 GACTATGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2568 GACTTTAAAGTC Eco47III 2164 AGCGCT AGC^GCT 3256 AGCGCT Hin1I 27 GGCGTC G[GA]^CG[CT]C 3405 GACGTC KpnI 1814 GGTACC GGTAC^C 3682 GGTACC MflI 262 AGATCT [GA]^GATC[CT] 2669 GGATCT PpuMI 2013 GGGACCT [GA]G^G[AT]CC[CT] 2125 AGGTCCC RcaI 86 TCATGA T^CATGA 2521 TCATGA SbfI 2198 CCTGCAGG CCTGCA^GG 2732 CCTGCAGG Sse8387I 2198 CCTGCAGG CCTGCA^GG 2732 CCTGCAGG XhoII 262 AGATCT [GA]^GATC[CT] 2669 GGATCT XmnI 260 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1158 GAACCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaII BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII EcoRV FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I