##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccI AccIII AclI AflIII AgeI
AhdI AocI AscI AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BglI BlnI
BsaAI BsaWI BseAI BsoBI BspEI
BspLU11I BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CpoI Eam1105I
Eco81I EcoO65I EcoRI EcoT14I EcoT22I
FseI FspI KasI KspI MfeI
MluI MluNI MroI MscI MunI
NarI NcoI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI StyI SwaI Tth111I Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3405 GACGTC GACGT^C
AflII 3926 CTTAAG C^TTAAG
ApaI 1750 GGGCCC GGGCC^C
AseI 2592 ATTAAT AT^TAAT
AsnI 2592 ATTAAT AT^TAAT
BcgI 931 CGAAAGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2057 GTATCC GTATCC(6/5)
BclI 1235 TGATCA T^GATCA
BfrI 3926 CTTAAG C^TTAAG
BglII 262 AGATCT A^GATCT
BlpI 1914 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1914 GCTCAGC GC^T[ACGT]AGC
BsaBI 387 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 362 GAGACC GGTCTC(1/5)
BsiEI 2740 CGGCCG CG[GA][CT]^CG
BsiWI 1362 CGTACG C^GTACG
BsmBI 3407 CGTCTC CGTCTC(1/5)
Bsp1407I 3802 TGTACA T^GTACA
BspDI 1447 ATCGAT AT^CGAT
BsrBI 2136 GAGCGG CCGCTC(-3/-3)
BsrGI 3802 TGTACA T^GTACA
BssHII 253 GCGCGC G^CGCGC
CelII 1914 GCTCAGC GC^T[ACGT]AGC
ClaI 1447 ATCGAT AT^CGAT
DraIII 1939 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3103 CCGTGG C^C[GA][CT]GG
EaeI 2740 CGGCCG [CT]^GGCC[GA]
EagI 2740 CGGCCG C^GGCCG
EclXI 2740 CGGCCG C^GGCCG
Eco52I 2740 CGGCCG C^GGCCG
FbaI 1235 TGATCA T^GATCA
HgaI 28 GCGTC GACGC(5/10)
HpaI 1487 GTTAAC GTT^AAC
MamI 387 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2222 GCCGGC GCC^GGC
NdeI 2052 CATATG CA^TATG
NgoMIV 2222 GCCGGC G^CCGGC
NheI 2888 GCTAGC G^CTAGC
PshBI 2592 ATTAAT AT^TAAT
SacI 2989 GAGCTC GAGCT^C
SapI 525 GAAGAGC GCTCTTC(1/4)
SexAI 1283 ACCTGGT A^CC[AT]GGT
SmlI 3926 CTTAAG C^T[CT][GA]AG
SspBI 3802 TGTACA T^GTACA
StuI 2863 AGGCCT AGG^CCT
XbaI 3769 TCTAGA T^CTAGA
XcmI 586 CCAAACGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1814 GGTACC G^GTACC
3682 GGTACC
AcyI 27 GGCGTC G[GA]^CG[CT]C
3405 GACGTC
Aor51HI 2164 AGCGCT AGC^GCT
3256 AGCGCT
Asp700I 260 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1158 GAACCAGTTC
Asp718I 1814 GGTACC G^GTACC
3682 GGTACC
BanI 1814 GGTACC G^G[CT][GA]CC
3682 GGTACC
BanII 1750 GGGCCC G[GA]GC[CT]^C
2989 GAGCTC
BpmI 2612 CTCCAG CTGGAG(16/14)
3721 CTCCAG
BsaHI 27 GGCGTC G[GA]^CG[CT]C
3405 GACGTC
BsgI 2083 CTGCAC GTGCAG(16/14)
2823 GTGCAG
BspHI 86 TCATGA T^CATGA
2521 TCATGA
BsrDI 2982 CATTGC GCAATG(2/0)
3916 GCAATG
BsrFI 43 GCCGGT [GA]^CCGG[CT]
2222 GCCGGC
BstAPI 2977 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3086 GCAGAAGCTGC
BstXI 2782 CCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3481 CCAGAACACTGG
BstYI 262 AGATCT [GA]^GATC[CT]
2669 GGATCT
Cfr10I 43 GCCGGT [GA]^CCGG[CT]
2222 GCCGGC
DraI 2571 TTTAAA TTT^AAA
4021 TTTAAA
DrdI 897 GACTATGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2568 GACTTTAAAGTC
Eco47III 2164 AGCGCT AGC^GCT
3256 AGCGCT
Hin1I 27 GGCGTC G[GA]^CG[CT]C
3405 GACGTC
KpnI 1814 GGTACC GGTAC^C
3682 GGTACC
MflI 262 AGATCT [GA]^GATC[CT]
2669 GGATCT
PpuMI 2013 GGGACCT [GA]G^G[AT]CC[CT]
2125 AGGTCCC
RcaI 86 TCATGA T^CATGA
2521 TCATGA
SbfI 2198 CCTGCAGG CCTGCA^GG
2732 CCTGCAGG
Sse8387I 2198 CCTGCAGG CCTGCA^GG
2732 CCTGCAGG
XhoII 262 AGATCT [GA]^GATC[CT]
2669 GGATCT
XmnI 260 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1158 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaII BbsI BbvI BcnI
BfaI BmyI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoNI EcoO109I EcoRII EcoRV FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I