##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AccI        AccIII      AclI        AflIII      AgeI        
AhdI        AocI        AscI        AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BglI        BlnI        
BsaAI       BsaWI       BseAI       BsoBI       BspEI       
BspLU11I    BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CpoI        Eam1105I    
Eco81I      EcoO65I     EcoRI       EcoT14I     EcoT22I     
FseI        FspI        KasI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NarI        NcoI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspI        StyI        SwaI        Tth111I     Van91I      
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        3405  GACGTC               GACGT^C
AflII        3926  CTTAAG               C^TTAAG
ApaI         1750  GGGCCC               GGGCC^C
AseI         2592  ATTAAT               AT^TAAT
AsnI         2592  ATTAAT               AT^TAAT
BcgI          931  CGAAAGATTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2057  GTATCC               GTATCC(6/5)
BclI         1235  TGATCA               T^GATCA
BfrI         3926  CTTAAG               C^TTAAG
BglII         262  AGATCT               A^GATCT
BlpI         1914  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1914  GCTCAGC              GC^T[ACGT]AGC
BsaBI         387  GATTATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          362  GAGACC               GGTCTC(1/5)
BsiEI        2740  CGGCCG               CG[GA][CT]^CG
BsiWI        1362  CGTACG               C^GTACG
BsmBI        3407  CGTCTC               CGTCTC(1/5)
Bsp1407I     3802  TGTACA               T^GTACA
BspDI        1447  ATCGAT               AT^CGAT
BsrBI        2136  GAGCGG               CCGCTC(-3/-3)
BsrGI        3802  TGTACA               T^GTACA
BssHII        253  GCGCGC               G^CGCGC
CelII        1914  GCTCAGC              GC^T[ACGT]AGC
ClaI         1447  ATCGAT               AT^CGAT
DraIII       1939  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         3103  CCGTGG               C^C[GA][CT]GG
EaeI         2740  CGGCCG               [CT]^GGCC[GA]
EagI         2740  CGGCCG               C^GGCCG
EclXI        2740  CGGCCG               C^GGCCG
Eco52I       2740  CGGCCG               C^GGCCG
FbaI         1235  TGATCA               T^GATCA
HgaI           28  GCGTC                GACGC(5/10)
HpaI         1487  GTTAAC               GTT^AAC
MamI          387  GATTATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         2222  GCCGGC               GCC^GGC
NdeI         2052  CATATG               CA^TATG
NgoMIV       2222  GCCGGC               G^CCGGC
NheI         2888  GCTAGC               G^CTAGC
PshBI        2592  ATTAAT               AT^TAAT
SacI         2989  GAGCTC               GAGCT^C
SapI          525  GAAGAGC              GCTCTTC(1/4)
SexAI        1283  ACCTGGT              A^CC[AT]GGT
SmlI         3926  CTTAAG               C^T[CT][GA]AG
SspBI        3802  TGTACA               T^GTACA
StuI         2863  AGGCCT               AGG^CCT
XbaI         3769  TCTAGA               T^CTAGA
XcmI          586  CCAAACGCTCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

Acc65I       1814  GGTACC               G^GTACC
             3682  GGTACC              
AcyI           27  GGCGTC               G[GA]^CG[CT]C
             3405  GACGTC              
Aor51HI      2164  AGCGCT               AGC^GCT
             3256  AGCGCT              
Asp700I       260  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1158  GAACCAGTTC          
Asp718I      1814  GGTACC               G^GTACC
             3682  GGTACC              
BanI         1814  GGTACC               G^G[CT][GA]CC
             3682  GGTACC              
BanII        1750  GGGCCC               G[GA]GC[CT]^C
             2989  GAGCTC              
BpmI         2612  CTCCAG               CTGGAG(16/14)
             3721  CTCCAG              
BsaHI          27  GGCGTC               G[GA]^CG[CT]C
             3405  GACGTC              
BsgI         2083  CTGCAC               GTGCAG(16/14)
             2823  GTGCAG              
BspHI          86  TCATGA               T^CATGA
             2521  TCATGA              
BsrDI        2982  CATTGC               GCAATG(2/0)
             3916  GCAATG              
BsrFI          43  GCCGGT               [GA]^CCGG[CT]
             2222  GCCGGC              
BstAPI       2977  GCAGCCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3086  GCAGAAGCTGC         
BstXI        2782  CCATCATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3481  CCAGAACACTGG        
BstYI         262  AGATCT               [GA]^GATC[CT]
             2669  GGATCT              
Cfr10I         43  GCCGGT               [GA]^CCGG[CT]
             2222  GCCGGC              
DraI         2571  TTTAAA               TTT^AAA
             4021  TTTAAA              
DrdI          897  GACTATGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2568  GACTTTAAAGTC        
Eco47III     2164  AGCGCT               AGC^GCT
             3256  AGCGCT              
Hin1I          27  GGCGTC               G[GA]^CG[CT]C
             3405  GACGTC              
KpnI         1814  GGTACC               GGTAC^C
             3682  GGTACC              
MflI          262  AGATCT               [GA]^GATC[CT]
             2669  GGATCT              
PpuMI        2013  GGGACCT              [GA]G^G[AT]CC[CT]
             2125  AGGTCCC             
RcaI           86  TCATGA               T^CATGA
             2521  TCATGA              
SbfI         2198  CCTGCAGG             CCTGCA^GG
             2732  CCTGCAGG            
Sse8387I     2198  CCTGCAGG             CCTGCA^GG
             2732  CCTGCAGG            
XhoII         262  AGATCT               [GA]^GATC[CT]
             2669  GGATCT              
XmnI          260  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1158  GAACCAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
AspHI       AvaII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpuAI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoNI       EcoO109I    EcoRII      EcoRV       FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I