##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AclI AcsI AflII ApoI
AseI AsnI Asp700I Asp718I AspI
BamHI BbeI BbrPI BclI BfrI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrGI BstAPI BstBI ClaI
CpoI DraI EcoRI EcoRV EcoT22I
FbaI FseI HindIII KasI KpnI
MamI MfeI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 964 GACGTC GACGT^C
AccI 544 GTATAC GT^[AC][GT]AC
AccIII 2073 TCCGGA T^CCGGA
AgeI 1540 ACCGGT A^CCGGT
AhdI 748 GACGGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2210 CCTCAGG CC^T[ACGT]AGG
Aor51HI 24 AGCGCT AGC^GCT
AscI 323 GGCGCGCC GG^CGCGCC
AspEI 748 GACGGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 116 TGCGCA TGC^GCA
AvrII 2863 CCTAGG C^CTAGG
BalI 1680 TGGCCA TGG^CCA
BciVI 560 GTATCC GTATCC(6/5)
BglII 1614 AGATCT A^GATCT
BlnI 2863 CCTAGG C^CTAGG
BlpI 39 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 39 GCTGAGC GC^T[ACGT]AGC
BseAI 2073 TCCGGA T^CCGGA
BsmBI 2194 GAGACG CGTCTC(1/5)
BspEI 2073 TCCGGA T^CCGGA
BspHI 1665 TCATGA T^CATGA
BsrDI 3116 GCAATG GCAATG(2/0)
Bst1107I 544 GTATAC GTA^TAC
BstYI 1614 AGATCT [GA]^GATC[CT]
BstZ17I 544 GTATAC GTA^TAC
Bsu36I 2210 CCTCAGG CC^T[ACGT]AGG
CelII 39 GCTGAGC GC^T[ACGT]AGC
Eam1105I 748 GACGGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 24 AGCGCT AGC^GCT
Eco81I 2210 CCTCAGG CC^T[ACGT]AGG
FspI 116 TGCGCA TGC^GCA
HpaI 1903 GTTAAC GTT^AAC
MflI 1614 AGATCT [GA]^GATC[CT]
MluI 80 ACGCGT A^CGCGT
MluNI 1680 TGGCCA TGG^CCA
MroI 2073 TCCGGA T^CCGGA
MscI 1680 TGGCCA TGG^CCA
NaeI 1117 GCCGGC GCC^GGC
NgoMIV 1117 GCCGGC G^CCGGC
PaeR7I 1931 CTCGAG C^TCGAG
PinAI 1540 ACCGGT A^CCGGT
RcaI 1665 TCATGA T^CATGA
XhoI 1931 CTCGAG C^TCGAG
XhoII 1614 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1160 GTGCAC G^TGCAC
2065 GTGCAC
ApaLI 1160 GTGCAC G^TGCAC
2065 GTGCAC
BcgI 98 GCAACGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2493 GCAGACACTTCG
BglI 1112 GCCCAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3100 GCCTTGCAGGC
BsaWI 1540 ACCGGT [AT]^CCGG[AT]
2073 TCCGGA
BsgI 16 CTGCAC GTGCAG(16/14)
2351 GTGCAG
BsiEI 221 CGGCCG CG[GA][CT]^CG
750 CGGCCG
BspMI 236 ACCTGC ACCTGC(4/8)
1187 ACCTGC
BsrBI 275 CCGCTC CCGCTC(-3/-3)
52 GAGCGG
BstEII 486 GGTGACC G^GT[ACGT]ACC
1128 GGTGACC
BstPI 486 GGTGACC G^GT[ACGT]ACC
1128 GGTGACC
DraIII 376 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1369 CACCTGGTG
DrdI 139 GACAGCCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2575 GACTTTCTGGTC
EagI 221 CGGCCG C^GGCCG
750 CGGCCG
EclXI 221 CGGCCG C^GGCCG
750 CGGCCG
Eco52I 221 CGGCCG C^GGCCG
750 CGGCCG
EcoNI 1317 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2163 CCTGAGCGAGG
EcoO65I 486 GGTGACC G^GT[ACGT]ACC
1128 GGTGACC
HaeII 24 AGCGCT [GA]GCGC^[CT]
33 GGCGCT
HincII 463 GTCAAC GT[CT]^[GA]AC
1903 GTTAAC
HindII 463 GTCAAC GT[CT]^[GA]AC
1903 GTTAAC
KspI 360 CCGCGG CCGC^GG
2282 CCGCGG
PstI 706 CTGCAG CTGCA^G
1967 CTGCAG
SacII 360 CCGCGG CCGC^GG
2282 CCGCGG
SapI 1956 GAAGAGC GCTCTTC(1/4)
204 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssHII BssKI BssSI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmaCI XmaI