##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AscI AseI AsnI AviII
BbeI BbrPI BcgI BfrI BglI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrFI BssHII
BssSI BstAPI BstBI Cfr10I ClaI
CpoI DraI EagI EclXI Eco52I
EcoNI EcoT22I FseI FspI Hin1I
HpaI KasI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspI StuI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2249 GGTACC G^GTACC
AccII 16 CGCG CG^CG
AhdI 1061 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1996 GTGCAC G^TGCAC
ApaLI 1996 GTGCAC G^TGCAC
Asp718I 2249 GGTACC G^GTACC
AspEI 1061 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3269 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3245 CCTAGG C^CTAGG
BbvCI 1600 CCTCAGC CCTCAGC(-5/-2)
BglII 2141 AGATCT A^GATCT
BlnI 3245 CCTAGG C^CTAGG
BlpI 1775 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1775 GCTCAGC GC^T[ACGT]AGC
BsaBI 2142 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2554 GTGCAG GTGCAG(16/14)
BsmI 384 GCATTC GAATGC(1/-1)
Bsp1407I 1097 TGTACA T^GTACA
BspHI 2435 TCATGA T^CATGA
BsrBI 2195 GAGCGG CCGCTC(-3/-3)
BsrGI 1097 TGTACA T^GTACA
Bst1107I 2394 GTATAC GTA^TAC
BstUI 16 CGCG CG^CG
BstZ17I 2394 GTATAC GTA^TAC
CelII 1775 GCTCAGC GC^T[ACGT]AGC
DraIII 2548 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3234 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1061 GACTGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3256 GAATTC G^AATTC
EcoRV 566 GATATC GAT^ATC
HincII 3215 GTCAAC GT[CT]^[GA]AC
HindII 3215 GTCAAC GT[CT]^[GA]AC
HindIII 269 AAGCTT A^AGCTT
KpnI 2249 GGTACC GGTAC^C
MamI 2142 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 16 CGCG CG^CG
PflFI 3269 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3442 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1285 CTGCAG CTGCA^G
RcaI 2435 TCATGA T^CATGA
SacI 2788 GAGCTC GAGCT^C
SapI 2714 GCTCTTC GCTCTTC(1/4)
SbfI 1284 CCTGCAGG CCTGCA^GG
ScaI 606 AGTACT AGT^ACT
SexAI 3051 ACCTGGT A^CC[AT]GGT
SphI 1044 GCATGC GCATG^C
Sse8387I 1284 CCTGCAGG CCTGCA^GG
SspBI 1097 TGTACA T^GTACA
Tth111I 3269 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3442 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2796 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 3256 GAATTC [GA]^AATT[CT]
3944 GAATTT
AflIII 1023 ACATGT A^C[GA][CT]GT
1829 ACATGT
Aor51HI 2027 AGCGCT AGC^GCT
2087 AGCGCT
ApaI 2543 GGGCCC GGGCC^C
2620 GGGCCC
ApoI 3256 GAATTC [GA]^AATT[CT]
3944 GAATTT
AvaI 681 CCCGAG C^[CT]CG[GA]G
1736 CTCGGG
BamHI 1034 GGATCC G^GATCC
2986 GGATCC
BanI 2249 GGTACC G^G[CT][GA]CC
2260 GGTGCC
BclI 470 TGATCA T^GATCA
1512 TGATCA
BsoBI 681 CCCGAG C^[CT]CG[GA]G
1736 CTCGGG
BspLU11I 1023 ACATGT A^CATGT
1829 ACATGT
BspMI 1283 ACCTGC ACCTGC(4/8)
2556 GCAGGT
BsrDI 1421 CATTGC GCAATG(2/0)
848 GCAATG
BstEII 1920 GGTCACC G^GT[ACGT]ACC
3815 GGTCACC
BstPI 1920 GGTCACC G^GT[ACGT]ACC
3815 GGTCACC
BstXI 928 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2085 CCAGCGCTTTGG
Eco47III 2027 AGCGCT AGC^GCT
2087 AGCGCT
EcoO65I 1920 GGTCACC G^GT[ACGT]ACC
3815 GGTCACC
FauI 778 CCCGC CCCGC(4/6)
3901 GCGGG
FbaI 470 TGATCA T^GATCA
1512 TGATCA
MaeII 985 ACGT A^CGT
3630 ACGT
MslI 1024 CATGTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1555 CATGCTGATG
MspA1I 839 CAGCTG C[AC]G^C[GT]G
2963 CAGCTG
PvuII 839 CAGCTG CAG^CTG
2963 CAGCTG
SfcI 989 CTGTAG C^T[GA][CT]AG
1285 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI Asp700I AspHI AvaII
BalI BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI