##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AspEI BamHI BbeI
BbrPI BcgI BsaBI BseAI BsiWI
BspDI BspEI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI KasI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I RsrII SacII SalI
SbfI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 22 GGTACC G^GTACC
AccII 1008 CGCG CG^CG
AcyI 2831 GGCGTC G[GA]^CG[CT]C
AflII 261 CTTAAG C^TTAAG
Alw44I 1358 GTGCAC G^TGCAC
ApaI 2732 GGGCCC GGGCC^C
ApaLI 1358 GTGCAC G^TGCAC
AseI 3659 ATTAAT AT^TAAT
AsnI 3659 ATTAAT AT^TAAT
Asp700I 1851 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 22 GGTACC G^GTACC
AvaI 1917 CTCGGG C^[CT]CG[GA]G
BbvCI 2305 CCTCAGC CCTCAGC(-5/-2)
BcnI 1830 CCGGG CC^[GC]GG
BfrI 261 CTTAAG C^TTAAG
BglII 3754 AGATCT A^GATCT
BsaHI 2831 GGCGTC G[GA]^CG[CT]C
BsaI 180 GGTCTC GGTCTC(1/5)
BsaWI 7 TCCGGT [AT]^CCGG[AT]
BsiEI 3035 CGATCG CG[GA][CT]^CG
BsmBI 1451 CGTCTC CGTCTC(1/5)
BsoBI 1917 CTCGGG C^[CT]CG[GA]G
BspLU11I 594 ACATGT A^CATGT
BsrBI 2990 GAGCGG CCGCTC(-3/-3)
BstAPI 945 GCAGAGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1008 CGCG CG^CG
DraIII 3637 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2523 CCATGG C^C[GA][CT]GG
EcoNI 3603 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2384 GAATTC G^AATTC
HaeII 2513 AGCGCC [GA]GCGC^[CT]
Hin1I 2831 GGCGTC G[GA]^CG[CT]C
HpaI 3492 GTTAAC GTT^AAC
KpnI 22 GGTACC GGTAC^C
MvnI 1008 CGCG CG^CG
NciI 1830 CCGGG CC^[GC]GG
NcoI 2523 CCATGG C^CATGG
PshBI 3659 ATTAAT AT^TAAT
PvuI 3035 CGATCG CGAT^CG
SacI 3264 GAGCTC GAGCT^C
ScaI 2802 AGTACT AGT^ACT
SfiI 3061 GGCCCGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 3543 AATATT AAT^ATT
XcmI 1797 CCACGACTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1851 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 1121 GATCC GGATC(4/5)
2622 GGATC
AviII 2712 TGCGCA TGC^GCA
3117 TGCGCA
BalI 2766 TGGCCA TGG^CCA
3069 TGGCCA
BciVI 545 GTATCC GTATCC(6/5)
2966 GTATCC
BsaAI 958 TACGTG [CT]AC^GT[GA]
2961 TACGTG
BsmI 713 GAATGC GAATGC(1/-1)
1059 GAATGC
Bsp1407I 3832 TGTACA T^GTACA
3855 TGTACA
BsrGI 3832 TGTACA T^GTACA
3855 TGTACA
DrdI 660 GACAAGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1955 GACCTTTCTGTC
EaeI 2766 TGGCCA [CT]^GGCC[GA]
3069 TGGCCA
FspI 2712 TGCGCA TGC^GCA
3117 TGCGCA
HincII 3029 GTTGAC GT[CT]^[GA]AC
3492 GTTAAC
HindII 3029 GTTGAC GT[CT]^[GA]AC
3492 GTTAAC
HindIII 563 AAGCTT A^AGCTT
3316 AAGCTT
MluNI 2766 TGGCCA TGG^CCA
3069 TGGCCA
MscI 2766 TGGCCA TGG^CCA
3069 TGGCCA
SapI 2939 GAAGAGC GCTCTTC(1/4)
2575 GCTCTTC
SexAI 2760 ACCAGGT A^CC[AT]GGT
3045 ACCAGGT
SphI 995 GCATGC GCATG^C
1112 GCATGC
SspBI 3832 TGTACA T^GTACA
3855 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwNI AocI ApoI AspHI
AspI AvaII AvrII BanI BanII
BbsI BbvI BclI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EarI Eco81I EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NheI
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII