##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AflII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
AviII BamHI BciVI BfrI BglII
BseAI BsiWI BsmBI BspEI BspHI
BsrDI BstAPI BstBI CpoI DrdI
Eam1105I EcoRI EcoRV EcoT22I FseI
FspI HindIII MfeI MluI MroI
MunI NdeI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI PvuI RcaI RsrII
SapI SbfI ScaI SfuI SmaI
SnaBI SphI Sse8387I SspI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 763 GACGTC GACGT^C
AclI 3059 AACGTT AA^CGTT
AcsI 1951 AAATTC [GA]^AATT[CT]
ApaI 91 GGGCCC GGGCC^C
ApoI 1951 AAATTC [GA]^AATT[CT]
Asp700I 2835 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2938 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1083 CACGTG CAC^GTG
BbvCI 3388 GCTGAGG CCTCAGC(-5/-2)
BcgI 890 CGATGAGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3301 TGATCA T^GATCA
BsaBI 3865 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 475 TCCGGT [AT]^CCGG[AT]
BseRI 2619 CTCCTC GAGGAG(10/8)
BsgI 2462 CTGCAC GTGCAG(16/14)
BspDI 1180 ATCGAT AT^CGAT
BsrBI 533 GAGCGG CCGCTC(-3/-3)
Bst1107I 2713 GTATAC GTA^TAC
BstEII 3997 GGTGACC G^GT[ACGT]ACC
BstPI 3997 GGTGACC G^GT[ACGT]ACC
BstZ17I 2713 GTATAC GTA^TAC
ClaI 1180 ATCGAT AT^CGAT
DraIII 2926 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3997 GGTGACC G^GT[ACGT]ACC
FbaI 3301 TGATCA T^GATCA
HpaI 3067 GTTAAC GTT^AAC
KspI 305 CCGCGG CCGC^GG
MamI 3865 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3237 CCATGG C^CATGG
PflFI 2938 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1083 CACGTG CAC^GTG
PmlI 1083 CACGTG CAC^GTG
Psp1406I 3059 AACGTT AA^CGTT
SacII 305 CCGCGG CCGC^GG
SalI 2239 GTCGAC G^TCGAC
SfiI 3147 GGCCATGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 799 CTTGAG C^T[CT][GA]AG
SpeI 3034 ACTAGT A^CTAGT
Tth111I 2938 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2835 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1044 GGTACC G^GTACC
1643 GGTACC
AlwNI 2587 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3796 CAGAGCCTG
Aor51HI 803 AGCGCT AGC^GCT
2417 AGCGCT
Asp718I 1044 GGTACC G^GTACC
1643 GGTACC
AvaI 530 CCCGAG C^[CT]CG[GA]G
1445 CCCGAG
AvrII 2293 CCTAGG C^CTAGG
3337 CCTAGG
BalI 729 TGGCCA TGG^CCA
2985 TGGCCA
BlnI 2293 CCTAGG C^CTAGG
3337 CCTAGG
BlpI 960 GCTCAGC GC^T[ACGT]AGC
3023 GCTCAGC
Bpu1102I 960 GCTCAGC GC^T[ACGT]AGC
3023 GCTCAGC
BsaAI 1083 CACGTG [CT]AC^GT[GA]
1855 TACGTG
BsiEI 536 CGGCCG CG[GA][CT]^CG
1818 CGGCCG
BsoBI 530 CCCGAG C^[CT]CG[GA]G
1445 CCCGAG
BstXI 2947 CCACTCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3882 CCAATTCTTTGG
CelII 960 GCTCAGC GC^T[ACGT]AGC
3023 GCTCAGC
EagI 536 CGGCCG C^GGCCG
1818 CGGCCG
EarI 2526 CTCTTC CTCTTC(1/4)
3013 GAAGAG
EclXI 536 CGGCCG C^GGCCG
1818 CGGCCG
Eco47III 803 AGCGCT AGC^GCT
2417 AGCGCT
Eco52I 536 CGGCCG C^GGCCG
1818 CGGCCG
EcoNI 114 CCTTCGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2244 CCTGGATGAGG
KpnI 1044 GGTACC GGTAC^C
1643 GGTACC
Ksp632I 2526 CTCTTC CTCTTC(1/4)
3013 GAAGAG
MluNI 729 TGGCCA TGG^CCA
2985 TGGCCA
MscI 729 TGGCCA TGG^CCA
2985 TGGCCA
NheI 6 GCTAGC G^CTAGC
1623 GCTAGC
PflMI 931 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1574 CCAGTGTGTGG
PpuMI 29 AGGACCC [GA]G^G[AT]CC[CT]
3835 AGGTCCT
SacI 958 GAGCTC GAGCT^C
1849 GAGCTC
SexAI 177 ACCTGGT A^CC[AT]GGT
2939 ACCTGGT
SgrAI 2123 CGCCGGTG C[GA]^CCGG[CT]G
2213 CGCCGGTG
Van91I 931 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1574 CCAGTGTGTGG
XbaI 483 TCTAGA T^CTAGA
3105 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AocI AspHI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspLU11I BspMI
BsrFI BsrGI BsrI BssHII BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII