################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AccI AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AspEI AvrII BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstXI BstZ17I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoNI FbaI FseI HincII HindII HindIII HpaI KasI MamI MfeI MluI MunI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 1358 GGTACC G^GTACC AccIII 1672 TCCGGA T^CCGGA AocI 492 CCTCAGG CC^T[ACGT]AGG Aor51HI 1119 AGCGCT AGC^GCT ApaI 16 GGGCCC GGGCC^C Asp700I 1347 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1358 GGTACC G^GTACC AspI 931 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2408 TGCGCA TGC^GCA BalI 2368 TGGCCA TGG^CCA BamHI 285 GGATCC G^GATCC BanI 1358 GGTACC G^G[CT][GA]CC BbvCI 1566 CCTCAGC CCTCAGC(-5/-2) BsaWI 1672 TCCGGA [AT]^CCGG[AT] BseAI 1672 TCCGGA T^CCGGA BsgI 557 CTGCAC GTGCAG(16/14) BsmI 1257 GCATTC GAATGC(1/-1) BspEI 1672 TCCGGA T^CCGGA BsrDI 952 GCAATG GCAATG(2/0) BstEII 1296 GGTTACC G^GT[ACGT]ACC BstPI 1296 GGTTACC G^GT[ACGT]ACC Bsu36I 492 CCTCAGG CC^T[ACGT]AGG DraI 1983 TTTAAA TTT^AAA DraIII 123 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2368 TGGCCA [CT]^GGCC[GA] Eco47III 1119 AGCGCT AGC^GCT Eco81I 492 CCTCAGG CC^T[ACGT]AGG EcoO65I 1296 GGTTACC G^GT[ACGT]ACC EcoRV 2178 GATATC GAT^ATC FspI 2408 TGCGCA TGC^GCA KpnI 1358 GGTACC GGTAC^C KspI 1142 CCGCGG CCGC^GG MluNI 2368 TGGCCA TGG^CCA MroI 1672 TCCGGA T^CCGGA MscI 2368 TGGCCA TGG^CCA NcoI 193 CCATGG C^CATGG NheI 2208 GCTAGC G^CTAGC PflFI 931 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 706 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 788 AGGACCT [GA]G^G[AT]CC[CT] PvuII 177 CAGCTG CAG^CTG SacI 1832 GAGCTC GAGCT^C SacII 1142 CCGCGG CCGC^GG ScaI 2703 AGTACT AGT^ACT SmaI 12 CCCGGG CCC^GGG StuI 1852 AGGCCT AGG^CCT Tth111I 931 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 706 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2431 TCTAGA T^CTAGA XmaCI 12 CCCGGG C^CCGGG XmaI 12 CCCGGG C^CCGGG XmnI 1347 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 547 GGCGTC G[GA]^CG[CT]C 735 GGCGTC AseI 1194 ATTAAT AT^TAAT 1968 ATTAAT AsnI 1194 ATTAAT AT^TAAT 1968 ATTAAT AvaI 12 CCCGGG C^[CT]CG[GA]G 1007 CCCGAG BcnI 12 CCCGG CC^[GC]GG 13 CCGGG BglII 1514 AGATCT A^GATCT 2846 AGATCT BsaHI 547 GGCGTC G[GA]^CG[CT]C 735 GGCGTC BsoBI 12 CCCGGG C^[CT]CG[GA]G 1007 CCCGAG BstBI 251 TTCGAA TT^CGAA 1338 TTCGAA DraII 15 GGGGCCC [GA]G^G[ACGT]CC[CT] 788 AGGACCT EcoO109I 15 GGGGCCC [GA]G^G[ACGT]CC[CT] 788 AGGACCT EcoRI 682 GAATTC G^AATTC 2055 GAATTC EcoT14I 193 CCATGG C^C[AT][AT]GG 2371 CCAAGG HaeII 40 GGCGCT [GA]GCGC^[CT] 1119 AGCGCT HapII 13 CCGG C^CGG 1673 CCGG HgaI 548 GCGTC GACGC(5/10) 736 GCGTC Hin1I 547 GGCGTC G[GA]^CG[CT]C 735 GGCGTC HpaII 13 CCGG C^CGG 1673 CCGG MaeII 645 ACGT A^CGT 2874 ACGT MslI 1999 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2656 CATCAGCATG MspA1I 177 CAGCTG C[AC]G^C[GT]G 1142 CCGCGG MspI 13 CCGG C^CGG 1673 CCGG NciI 12 CCCGG CC^[GC]GG 13 CCGGG NdeI 1999 CATATG CA^TATG 2747 CATATG NspI 298 GCATGC [GA]CATG^[CT] 2661 GCATGC NspV 251 TTCGAA TT^CGAA 1338 TTCGAA PshBI 1194 ATTAAT AT^TAAT 1968 ATTAAT SfuI 251 TTCGAA TT^CGAA 1338 TTCGAA SphI 298 GCATGC GCATG^C 2661 GCATGC StyI 193 CCATGG C^C[AT][AT]GG 2371 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT22I FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MvnI MwoI NdeII NlaIII NlaIV NsiI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII