##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
AspEI AvrII BbeI BbrPI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI FbaI FseI HincII HindII
HindIII HpaI KasI MamI MfeI
MluI MunI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1358 GGTACC G^GTACC
AccIII 1672 TCCGGA T^CCGGA
AocI 492 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1119 AGCGCT AGC^GCT
ApaI 16 GGGCCC GGGCC^C
Asp700I 1347 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1358 GGTACC G^GTACC
AspI 931 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2408 TGCGCA TGC^GCA
BalI 2368 TGGCCA TGG^CCA
BamHI 285 GGATCC G^GATCC
BanI 1358 GGTACC G^G[CT][GA]CC
BbvCI 1566 CCTCAGC CCTCAGC(-5/-2)
BsaWI 1672 TCCGGA [AT]^CCGG[AT]
BseAI 1672 TCCGGA T^CCGGA
BsgI 557 CTGCAC GTGCAG(16/14)
BsmI 1257 GCATTC GAATGC(1/-1)
BspEI 1672 TCCGGA T^CCGGA
BsrDI 952 GCAATG GCAATG(2/0)
BstEII 1296 GGTTACC G^GT[ACGT]ACC
BstPI 1296 GGTTACC G^GT[ACGT]ACC
Bsu36I 492 CCTCAGG CC^T[ACGT]AGG
DraI 1983 TTTAAA TTT^AAA
DraIII 123 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2368 TGGCCA [CT]^GGCC[GA]
Eco47III 1119 AGCGCT AGC^GCT
Eco81I 492 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1296 GGTTACC G^GT[ACGT]ACC
EcoRV 2178 GATATC GAT^ATC
FspI 2408 TGCGCA TGC^GCA
KpnI 1358 GGTACC GGTAC^C
KspI 1142 CCGCGG CCGC^GG
MluNI 2368 TGGCCA TGG^CCA
MroI 1672 TCCGGA T^CCGGA
MscI 2368 TGGCCA TGG^CCA
NcoI 193 CCATGG C^CATGG
NheI 2208 GCTAGC G^CTAGC
PflFI 931 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 706 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 788 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 177 CAGCTG CAG^CTG
SacI 1832 GAGCTC GAGCT^C
SacII 1142 CCGCGG CCGC^GG
ScaI 2703 AGTACT AGT^ACT
SmaI 12 CCCGGG CCC^GGG
StuI 1852 AGGCCT AGG^CCT
Tth111I 931 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 706 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2431 TCTAGA T^CTAGA
XmaCI 12 CCCGGG C^CCGGG
XmaI 12 CCCGGG C^CCGGG
XmnI 1347 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 547 GGCGTC G[GA]^CG[CT]C
735 GGCGTC
AseI 1194 ATTAAT AT^TAAT
1968 ATTAAT
AsnI 1194 ATTAAT AT^TAAT
1968 ATTAAT
AvaI 12 CCCGGG C^[CT]CG[GA]G
1007 CCCGAG
BcnI 12 CCCGG CC^[GC]GG
13 CCGGG
BglII 1514 AGATCT A^GATCT
2846 AGATCT
BsaHI 547 GGCGTC G[GA]^CG[CT]C
735 GGCGTC
BsoBI 12 CCCGGG C^[CT]CG[GA]G
1007 CCCGAG
BstBI 251 TTCGAA TT^CGAA
1338 TTCGAA
DraII 15 GGGGCCC [GA]G^G[ACGT]CC[CT]
788 AGGACCT
EcoO109I 15 GGGGCCC [GA]G^G[ACGT]CC[CT]
788 AGGACCT
EcoRI 682 GAATTC G^AATTC
2055 GAATTC
EcoT14I 193 CCATGG C^C[AT][AT]GG
2371 CCAAGG
HaeII 40 GGCGCT [GA]GCGC^[CT]
1119 AGCGCT
HapII 13 CCGG C^CGG
1673 CCGG
HgaI 548 GCGTC GACGC(5/10)
736 GCGTC
Hin1I 547 GGCGTC G[GA]^CG[CT]C
735 GGCGTC
HpaII 13 CCGG C^CGG
1673 CCGG
MaeII 645 ACGT A^CGT
2874 ACGT
MslI 1999 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2656 CATCAGCATG
MspA1I 177 CAGCTG C[AC]G^C[GT]G
1142 CCGCGG
MspI 13 CCGG C^CGG
1673 CCGG
NciI 12 CCCGG CC^[GC]GG
13 CCGGG
NdeI 1999 CATATG CA^TATG
2747 CATATG
NspI 298 GCATGC [GA]CATG^[CT]
2661 GCATGC
NspV 251 TTCGAA TT^CGAA
1338 TTCGAA
PshBI 1194 ATTAAT AT^TAAT
1968 ATTAAT
SfuI 251 TTCGAA TT^CGAA
1338 TTCGAA
SphI 298 GCATGC GCATG^C
2661 GCATGC
StyI 193 CCATGG C^C[AT][AT]GG
2371 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRII
EcoT22I FauI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MvnI
MwoI NdeII NlaIII NlaIV NsiI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII