################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI Aor51HI AscI AseI AsnI Asp718I AviII AvrII BbeI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrGI BssHII Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MamI MluI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SwaI Van91I XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 725 GTCTAC GT^[AC][GT]AC AccIII 1825 TCCGGA T^CCGGA AhdI 3536 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1941 GTGCAC G^TGCAC ApaLI 1941 GTGCAC G^TGCAC AspEI 3536 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BglI 1148 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1682 AGATCT A^GATCT BsaWI 1825 TCCGGA [AT]^CCGG[AT] BseAI 1825 TCCGGA T^CCGGA BspEI 1825 TCCGGA T^CCGGA BspHI 675 TCATGA T^CATGA BsrBI 548 GAGCGG CCGCTC(-3/-3) BsrFI 2840 GCCGGT [GA]^CCGG[CT] BstEII 3261 GGTCACC G^GT[ACGT]ACC BstPI 3261 GGTCACC G^GT[ACGT]ACC Cfr10I 2840 GCCGGT [GA]^CCGG[CT] Eam1105I 3536 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 95 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3261 GGTCACC G^GT[ACGT]ACC EcoT22I 1514 ATGCAT ATGCA^T FauI 3023 CCCGC CCCGC(4/6) MfeI 2097 CAATTG C^AATTG MroI 1825 TCCGGA T^CCGGA MunI 2097 CAATTG C^AATTG NcoI 858 CCATGG C^CATGG NsiI 1514 ATGCAT ATGCA^T PshAI 2585 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 675 TCATGA T^CATGA SbfI 2219 CCTGCAGG CCTGCA^GG ScaI 1219 AGTACT AGT^ACT SmaI 1867 CCCGGG CCC^GGG SmlI 4163 CTCAAG C^T[CT][GA]AG Sse8387I 2219 CCTGCAGG CCTGCA^GG TaqI 1266 TCGA T^CGA TthHB8I 1266 TCGA T^CGA XbaI 2255 TCTAGA T^CTAGA XmaCI 1867 CCCGGG C^CCGGG XmaI 1867 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 764 ACGTGT A^C[GA][CT]GT 1937 ACACGT AocI 2796 CCTCAGG CC^T[ACGT]AGG 3122 CCTTAGG ApaI 1616 GGGCCC GGGCC^C 2601 GGGCCC Asp700I 1025 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3916 GAATGGCTTC AspI 2020 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2464 GACTCAGTC AvaI 1867 CCCGGG C^[CT]CG[GA]G 2665 CTCGGG BalI 1561 TGGCCA TGG^CCA 2692 TGGCCA BamHI 2809 GGATCC G^GATCC 3118 GGATCC BsoBI 1867 CCCGGG C^[CT]CG[GA]G 2665 CTCGGG BssSI 1611 CTCGTG CACGAG(-5/-1) 2804 CTCGTG BstXI 525 CCAGCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1741 CCAGGAAAGTGG Bsu36I 2796 CCTCAGG CC^T[ACGT]AGG 3122 CCTTAGG CfoI 175 GCGC GCG^C 3604 GCGC Eco81I 2796 CCTCAGG CC^T[ACGT]AGG 3122 CCTTAGG HhaI 175 GCGC GCG^C 3604 GCGC HinP1I 175 GCGC G^CGC 3604 GCGC HincII 1234 GTTGAC GT[CT]^[GA]AC 4070 GTTGAC HindII 1234 GTTGAC GT[CT]^[GA]AC 4070 GTTGAC MluNI 1561 TGGCCA TGG^CCA 2692 TGGCCA MscI 1561 TGGCCA TGG^CCA 2692 TGGCCA PflFI 2020 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2464 GACTCAGTC PvuII 1328 CAGCTG CAG^CTG 2457 CAGCTG SexAI 680 ACCAGGT A^CC[AT]GGT 3462 ACCAGGT SspI 3785 AATATT AAT^ATT 4150 AATATT Tth111I 2020 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2464 GACTCAGTC XmnI 1025 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3916 GAATGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbrPI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII