##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI Aor51HI AscI AseI
AsnI Asp718I AviII AvrII BbeI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MamI
MluI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SwaI
Van91I XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 725 GTCTAC GT^[AC][GT]AC
AccIII 1825 TCCGGA T^CCGGA
AhdI 3536 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1941 GTGCAC G^TGCAC
ApaLI 1941 GTGCAC G^TGCAC
AspEI 3536 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI 1148 GCCACACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1682 AGATCT A^GATCT
BsaWI 1825 TCCGGA [AT]^CCGG[AT]
BseAI 1825 TCCGGA T^CCGGA
BspEI 1825 TCCGGA T^CCGGA
BspHI 675 TCATGA T^CATGA
BsrBI 548 GAGCGG CCGCTC(-3/-3)
BsrFI 2840 GCCGGT [GA]^CCGG[CT]
BstEII 3261 GGTCACC G^GT[ACGT]ACC
BstPI 3261 GGTCACC G^GT[ACGT]ACC
Cfr10I 2840 GCCGGT [GA]^CCGG[CT]
Eam1105I 3536 GACTCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 95 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3261 GGTCACC G^GT[ACGT]ACC
EcoT22I 1514 ATGCAT ATGCA^T
FauI 3023 CCCGC CCCGC(4/6)
MfeI 2097 CAATTG C^AATTG
MroI 1825 TCCGGA T^CCGGA
MunI 2097 CAATTG C^AATTG
NcoI 858 CCATGG C^CATGG
NsiI 1514 ATGCAT ATGCA^T
PshAI 2585 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 675 TCATGA T^CATGA
SbfI 2219 CCTGCAGG CCTGCA^GG
ScaI 1219 AGTACT AGT^ACT
SmaI 1867 CCCGGG CCC^GGG
SmlI 4163 CTCAAG C^T[CT][GA]AG
Sse8387I 2219 CCTGCAGG CCTGCA^GG
TaqI 1266 TCGA T^CGA
TthHB8I 1266 TCGA T^CGA
XbaI 2255 TCTAGA T^CTAGA
XmaCI 1867 CCCGGG C^CCGGG
XmaI 1867 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 764 ACGTGT A^C[GA][CT]GT
1937 ACACGT
AocI 2796 CCTCAGG CC^T[ACGT]AGG
3122 CCTTAGG
ApaI 1616 GGGCCC GGGCC^C
2601 GGGCCC
Asp700I 1025 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3916 GAATGGCTTC
AspI 2020 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2464 GACTCAGTC
AvaI 1867 CCCGGG C^[CT]CG[GA]G
2665 CTCGGG
BalI 1561 TGGCCA TGG^CCA
2692 TGGCCA
BamHI 2809 GGATCC G^GATCC
3118 GGATCC
BsoBI 1867 CCCGGG C^[CT]CG[GA]G
2665 CTCGGG
BssSI 1611 CTCGTG CACGAG(-5/-1)
2804 CTCGTG
BstXI 525 CCAGCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1741 CCAGGAAAGTGG
Bsu36I 2796 CCTCAGG CC^T[ACGT]AGG
3122 CCTTAGG
CfoI 175 GCGC GCG^C
3604 GCGC
Eco81I 2796 CCTCAGG CC^T[ACGT]AGG
3122 CCTTAGG
HhaI 175 GCGC GCG^C
3604 GCGC
HinP1I 175 GCGC G^CGC
3604 GCGC
HincII 1234 GTTGAC GT[CT]^[GA]AC
4070 GTTGAC
HindII 1234 GTTGAC GT[CT]^[GA]AC
4070 GTTGAC
MluNI 1561 TGGCCA TGG^CCA
2692 TGGCCA
MscI 1561 TGGCCA TGG^CCA
2692 TGGCCA
PflFI 2020 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2464 GACTCAGTC
PvuII 1328 CAGCTG CAG^CTG
2457 CAGCTG
SexAI 680 ACCAGGT A^CC[AT]GGT
3462 ACCAGGT
SspI 3785 AATATT AAT^ATT
4150 AATATT
Tth111I 2020 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2464 GACTCAGTC
XmnI 1025 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3916 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PstI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII