##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AviII       AvrII       BbeI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstUI       BstZ17I     CelII       ClaI        
CpoI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HpaI        KasI        KpnI        KspI        MamI        
MluI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SspBI       SwaI        
Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          725  GTCTAC               GT^[AC][GT]AC
AccIII       1825  TCCGGA               T^CCGGA
AhdI         3536  GACTCAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1941  GTGCAC               G^TGCAC
ApaLI        1941  GTGCAC               G^TGCAC
AspEI        3536  GACTCAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI         1148  GCCACACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1682  AGATCT               A^GATCT
BsaWI        1825  TCCGGA               [AT]^CCGG[AT]
BseAI        1825  TCCGGA               T^CCGGA
BspEI        1825  TCCGGA               T^CCGGA
BspHI         675  TCATGA               T^CATGA
BsrBI         548  GAGCGG               CCGCTC(-3/-3)
BsrFI        2840  GCCGGT               [GA]^CCGG[CT]
BstEII       3261  GGTCACC              G^GT[ACGT]ACC
BstPI        3261  GGTCACC              G^GT[ACGT]ACC
Cfr10I       2840  GCCGGT               [GA]^CCGG[CT]
Eam1105I     3536  GACTCAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI          95  CCTTCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3261  GGTCACC              G^GT[ACGT]ACC
EcoT22I      1514  ATGCAT               ATGCA^T
FauI         3023  CCCGC                CCCGC(4/6)
MfeI         2097  CAATTG               C^AATTG
MroI         1825  TCCGGA               T^CCGGA
MunI         2097  CAATTG               C^AATTG
NcoI          858  CCATGG               C^CATGG
NsiI         1514  ATGCAT               ATGCA^T
PshAI        2585  GACCATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          675  TCATGA               T^CATGA
SbfI         2219  CCTGCAGG             CCTGCA^GG
ScaI         1219  AGTACT               AGT^ACT
SmaI         1867  CCCGGG               CCC^GGG
SmlI         4163  CTCAAG               C^T[CT][GA]AG
Sse8387I     2219  CCTGCAGG             CCTGCA^GG
TaqI         1266  TCGA                 T^CGA
TthHB8I      1266  TCGA                 T^CGA
XbaI         2255  TCTAGA               T^CTAGA
XmaCI        1867  CCCGGG               C^CCGGG
XmaI         1867  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AflIII        764  ACGTGT               A^C[GA][CT]GT
             1937  ACACGT              
AocI         2796  CCTCAGG              CC^T[ACGT]AGG
             3122  CCTTAGG             
ApaI         1616  GGGCCC               GGGCC^C
             2601  GGGCCC              
Asp700I      1025  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3916  GAATGGCTTC          
AspI         2020  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2464  GACTCAGTC           
AvaI         1867  CCCGGG               C^[CT]CG[GA]G
             2665  CTCGGG              
BalI         1561  TGGCCA               TGG^CCA
             2692  TGGCCA              
BamHI        2809  GGATCC               G^GATCC
             3118  GGATCC              
BsoBI        1867  CCCGGG               C^[CT]CG[GA]G
             2665  CTCGGG              
BssSI        1611  CTCGTG               CACGAG(-5/-1)
             2804  CTCGTG              
BstXI         525  CCAGCGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1741  CCAGGAAAGTGG        
Bsu36I       2796  CCTCAGG              CC^T[ACGT]AGG
             3122  CCTTAGG             
CfoI          175  GCGC                 GCG^C
             3604  GCGC                
Eco81I       2796  CCTCAGG              CC^T[ACGT]AGG
             3122  CCTTAGG             
HhaI          175  GCGC                 GCG^C
             3604  GCGC                
HinP1I        175  GCGC                 G^CGC
             3604  GCGC                
HincII       1234  GTTGAC               GT[CT]^[GA]AC
             4070  GTTGAC              
HindII       1234  GTTGAC               GT[CT]^[GA]AC
             4070  GTTGAC              
MluNI        1561  TGGCCA               TGG^CCA
             2692  TGGCCA              
MscI         1561  TGGCCA               TGG^CCA
             2692  TGGCCA              
PflFI        2020  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2464  GACTCAGTC           
PvuII        1328  CAGCTG               CAG^CTG
             2457  CAGCTG              
SexAI         680  ACCAGGT              A^CC[AT]GGT
             3462  ACCAGGT             
SspI         3785  AATATT               AAT^ATT
             4150  AATATT              
Tth111I      2020  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2464  GACTCAGTC           
XmnI         1025  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3916  GAATGGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BanI        
BanII       BbrPI       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PmaCI       
PmlI        PpuMI       PstI        RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StuI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XcmI        
XhoII