##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AclI AflII AflIII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AviII BbeI BbrPI BclI
BfrI BsaAI BsiEI BsiWI BsmBI
BspDI BspLU11I BspMI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
FbaI FseI FspI HpaI KasI
KspI MaeII MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1383 GGTACC G^GTACC
AccIII 659 TCCGGA T^CCGGA
AcyI 3252 GACGCC G[GA]^CG[CT]C
AgeI 1487 ACCGGT A^CCGGT
AocI 325 CCTCAGG CC^T[ACGT]AGG
Asp700I 962 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1383 GGTACC G^GTACC
AspI 720 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1550 CCTAGG C^CTAGG
BcgI 294 GCAAACAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3698 GTATCC GTATCC(6/5)
BglI 2572 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1550 CCTAGG C^CTAGG
BlpI 475 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 475 GCTCAGC GC^T[ACGT]AGC
BsaHI 3252 GACGCC G[GA]^CG[CT]C
BsaI 3671 GAGACC GGTCTC(1/5)
BseAI 659 TCCGGA T^CCGGA
Bsp1407I 1740 TGTACA T^GTACA
BspEI 659 TCCGGA T^CCGGA
BsrBI 738 CCGCTC CCGCTC(-3/-3)
BsrGI 1740 TGTACA T^GTACA
BstAPI 560 GCAACACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 325 CCTCAGG CC^T[ACGT]AGG
CelII 475 GCTCAGC GC^T[ACGT]AGC
DrdI 3167 GACAATAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 325 CCTCAGG CC^T[ACGT]AGG
EcoNI 2830 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1992 ATGCAT ATGCA^T
HgaI 3252 GACGC GACGC(5/10)
Hin1I 3252 GACGCC G[GA]^CG[CT]C
HindIII 1270 AAGCTT A^AGCTT
KpnI 1383 GGTACC GGTAC^C
MroI 659 TCCGGA T^CCGGA
NsiI 1992 ATGCAT ATGCA^T
PflFI 720 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1487 ACCGGT A^CCGGT
PstI 2606 CTGCAG CTGCA^G
SacI 649 GAGCTC GAGCT^C
SalI 3249 GTCGAC G^TCGAC
SpeI 285 ACTAGT A^CTAGT
SspBI 1740 TGTACA T^GTACA
StuI 695 AGGCCT AGG^CCT
Tth111I 720 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 962 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 338 CGCG CG^CG
2558 CGCG
AcsI 2138 GAATTT [GA]^AATT[CT]
2955 AAATTT
ApoI 2138 GAATTT [GA]^AATT[CT]
2955 AAATTT
AseI 1873 ATTAAT AT^TAAT
1914 ATTAAT
AsnI 1873 ATTAAT AT^TAAT
1914 ATTAAT
AspHI 473 GTGCTC G[AT]GC[AT]^C
649 GAGCTC
BamHI 1125 GGATCC G^GATCC
1287 GGATCC
BanI 1383 GGTACC G^G[CT][GA]CC
1457 GGCACC
BbvCI 691 GCTGAGG CCTCAGC(-5/-2)
2732 GCTGAGG
BglII 705 AGATCT A^GATCT
1185 AGATCT
BsaBI 859 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2529 GATGCCCATC
BsgI 2786 GTGCAG GTGCAG(16/14)
2984 GTGCAG
BsiHKAI 473 GTGCTC G[AT]GC[AT]^C
649 GAGCTC
BsmI 182 GCATTC GAATGC(1/-1)
2186 GCATTC
BspHI 572 TCATGA T^CATGA
2021 TCATGA
BsrFI 1353 GCCGGT [GA]^CCGG[CT]
1487 ACCGGT
BstUI 338 CGCG CG^CG
2558 CGCG
Cfr10I 1353 GCCGGT [GA]^CCGG[CT]
1487 ACCGGT
DraIII 3650 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3898 CACTGTGTG
EcoRV 1318 GATATC GAT^ATC
3329 GATATC
FauI 1130 CCCGC CCCGC(4/6)
1398 GCGGG
HaeII 96 AGCGCC [GA]GCGC^[CT]
3234 AGCGCC
HincII 3249 GTCGAC GT[CT]^[GA]AC
3791 GTTGAC
HindII 3249 GTCGAC GT[CT]^[GA]AC
3791 GTTGAC
MamI 859 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2529 GATGCCCATC
MvnI 338 CGCG CG^CG
2558 CGCG
PpuMI 2474 GGGACCC [GA]G^G[AT]CC[CT]
3000 GGGACCT
PshAI 1583 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3252 GACGCCAGTC
PshBI 1873 ATTAAT AT^TAAT
1914 ATTAAT
RcaI 572 TCATGA T^CATGA
2021 TCATGA
SmaI 1481 CCCGGG CCC^GGG
2961 CCCGGG
SmlI 415 CTTGAG C^T[CT][GA]AG
2110 CTTGAG
SphI 2051 GCATGC GCATG^C
3099 GCATGC
XmaCI 1481 CCCGGG C^CCGGG
2961 CCCGGG
XmaI 1481 CCCGGG C^CCGGG
2961 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII