################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AgeI Aor51HI ApaI AscI Asp700I Asp718I AspI AvrII BbeI BbrPI BcgI BglI BlnI BsaAI BsaBI BseAI BsmI BspDI BspEI BspMI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DrdI Eco47III EcoRI EcoT22I FseI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 196 GACGTC GACGT^C AcyI 196 GACGTC G[GA]^CG[CT]C AflII 2980 CTTAAG C^TTAAG AflIII 77 ACATGT A^C[GA][CT]GT Alw44I 2779 GTGCAC G^TGCAC AlwNI 2992 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 3180 CCTGAGG CC^T[ACGT]AGG ApaLI 2779 GTGCAC G^TGCAC AseI 3451 ATTAAT AT^TAAT AsnI 3451 ATTAAT AT^TAAT AviII 308 TGCGCA TGC^GCA BciVI 1857 GTATCC GTATCC(6/5) BclI 729 TGATCA T^GATCA BfrI 2980 CTTAAG C^TTAAG BglII 534 AGATCT A^GATCT BsaHI 196 GACGTC G[GA]^CG[CT]C BsaWI 677 ACCGGA [AT]^CCGG[AT] BsiEI 3042 CGGCCG CG[GA][CT]^CG BsiWI 2920 CGTACG C^GTACG BspHI 3552 TCATGA T^CATGA BspLU11I 77 ACATGT A^CATGT BsrBI 2793 GAGCGG CCGCTC(-3/-3) BsrDI 899 CATTGC GCAATG(2/0) BstEII 1338 GGTCACC G^GT[ACGT]ACC BstPI 1338 GGTCACC G^GT[ACGT]ACC Bsu36I 3180 CCTGAGG CC^T[ACGT]AGG DraI 3426 TTTAAA TTT^AAA EagI 3042 CGGCCG C^GGCCG EclXI 3042 CGGCCG C^GGCCG Eco52I 3042 CGGCCG C^GGCCG Eco81I 3180 CCTGAGG CC^T[ACGT]AGG EcoNI 3001 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1338 GGTCACC G^GT[ACGT]ACC EcoRV 998 GATATC GAT^ATC FauI 3011 GCGGG CCCGC(4/6) FbaI 729 TGATCA T^GATCA FspI 308 TGCGCA TGC^GCA HaeII 2696 GGCGCT [GA]GCGC^[CT] HgaI 228 GCGTC GACGC(5/10) Hin1I 196 GACGTC G[GA]^CG[CT]C NheI 3199 GCTAGC G^CTAGC PshAI 1883 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3451 ATTAAT AT^TAAT RcaI 3552 TCATGA T^CATGA SacI 1129 GAGCTC GAGCT^C ScaI 2363 AGTACT AGT^ACT SspI 969 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AhdI 249 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1196 GACCTCCTGTC AspEI 249 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1196 GACCTCCTGTC AvaI 977 CCCGAG C^[CT]CG[GA]G 1244 CCCGAG BalI 261 TGGCCA TGG^CCA 2760 TGGCCA BamHI 776 GGATCC G^GATCC 3185 GGATCC BanI 1315 GGTGCC G^G[CT][GA]CC 2962 GGTGCC BlpI 1086 GCTGAGC GC^T[ACGT]AGC 2251 GCTCAGC Bpu1102I 1086 GCTGAGC GC^T[ACGT]AGC 2251 GCTCAGC BsaI 982 GAGACC GGTCTC(1/5) 2510 GAGACC BsmBI 3624 CGTCTC CGTCTC(1/5) 243 GAGACG BsoBI 977 CCCGAG C^[CT]CG[GA]G 1244 CCCGAG Bsp1407I 2516 TGTACA T^GTACA 3068 TGTACA BsrFI 985 ACCGGC [GA]^CCGG[CT] 1512 GCCGGT BsrGI 2516 TGTACA T^GTACA 3068 TGTACA BssSI 771 CACGAG CACGAG(-5/-1) 2607 CACGAG CelII 1086 GCTGAGC GC^T[ACGT]AGC 2251 GCTCAGC CfoI 309 GCGC GCG^C 2697 GCGC Cfr10I 985 ACCGGC [GA]^CCGG[CT] 1512 GCCGGT Eam1105I 249 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1196 GACCTCCTGTC HhaI 309 GCGC GCG^C 2697 GCGC HinP1I 309 GCGC G^CGC 2697 GCGC HincII 1922 GTCAAC GT[CT]^[GA]AC 2766 GTCAAC HindII 1922 GTCAAC GT[CT]^[GA]AC 2766 GTCAAC MluNI 261 TGGCCA TGG^CCA 2760 TGGCCA MscI 261 TGGCCA TGG^CCA 2760 TGGCCA NcoI 2450 CCATGG C^CATGG 2757 CCATGG SexAI 1334 ACCAGGT A^CC[AT]GGT 2388 ACCAGGT SphI 1190 GCATGC GCATG^C 2000 GCATGC SspBI 2516 TGTACA T^GTACA 3068 TGTACA StuI 2076 AGGCCT AGG^CCT 2202 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII