##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AgeI Aor51HI ApaI AscI Asp700I
Asp718I AspI AvrII BbeI BbrPI
BcgI BglI BlnI BsaAI BsaBI
BseAI BsmI BspDI BspEI BspMI
BssHII Bst1107I BstBI BstUI BstZ17I
ClaI CpoI DrdI Eco47III EcoRI
EcoT22I FseI HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 196 GACGTC GACGT^C
AcyI 196 GACGTC G[GA]^CG[CT]C
AflII 2980 CTTAAG C^TTAAG
AflIII 77 ACATGT A^C[GA][CT]GT
Alw44I 2779 GTGCAC G^TGCAC
AlwNI 2992 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3180 CCTGAGG CC^T[ACGT]AGG
ApaLI 2779 GTGCAC G^TGCAC
AseI 3451 ATTAAT AT^TAAT
AsnI 3451 ATTAAT AT^TAAT
AviII 308 TGCGCA TGC^GCA
BciVI 1857 GTATCC GTATCC(6/5)
BclI 729 TGATCA T^GATCA
BfrI 2980 CTTAAG C^TTAAG
BglII 534 AGATCT A^GATCT
BsaHI 196 GACGTC G[GA]^CG[CT]C
BsaWI 677 ACCGGA [AT]^CCGG[AT]
BsiEI 3042 CGGCCG CG[GA][CT]^CG
BsiWI 2920 CGTACG C^GTACG
BspHI 3552 TCATGA T^CATGA
BspLU11I 77 ACATGT A^CATGT
BsrBI 2793 GAGCGG CCGCTC(-3/-3)
BsrDI 899 CATTGC GCAATG(2/0)
BstEII 1338 GGTCACC G^GT[ACGT]ACC
BstPI 1338 GGTCACC G^GT[ACGT]ACC
Bsu36I 3180 CCTGAGG CC^T[ACGT]AGG
DraI 3426 TTTAAA TTT^AAA
EagI 3042 CGGCCG C^GGCCG
EclXI 3042 CGGCCG C^GGCCG
Eco52I 3042 CGGCCG C^GGCCG
Eco81I 3180 CCTGAGG CC^T[ACGT]AGG
EcoNI 3001 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1338 GGTCACC G^GT[ACGT]ACC
EcoRV 998 GATATC GAT^ATC
FauI 3011 GCGGG CCCGC(4/6)
FbaI 729 TGATCA T^GATCA
FspI 308 TGCGCA TGC^GCA
HaeII 2696 GGCGCT [GA]GCGC^[CT]
HgaI 228 GCGTC GACGC(5/10)
Hin1I 196 GACGTC G[GA]^CG[CT]C
NheI 3199 GCTAGC G^CTAGC
PshAI 1883 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3451 ATTAAT AT^TAAT
RcaI 3552 TCATGA T^CATGA
SacI 1129 GAGCTC GAGCT^C
ScaI 2363 AGTACT AGT^ACT
SspI 969 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AhdI 249 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1196 GACCTCCTGTC
AspEI 249 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1196 GACCTCCTGTC
AvaI 977 CCCGAG C^[CT]CG[GA]G
1244 CCCGAG
BalI 261 TGGCCA TGG^CCA
2760 TGGCCA
BamHI 776 GGATCC G^GATCC
3185 GGATCC
BanI 1315 GGTGCC G^G[CT][GA]CC
2962 GGTGCC
BlpI 1086 GCTGAGC GC^T[ACGT]AGC
2251 GCTCAGC
Bpu1102I 1086 GCTGAGC GC^T[ACGT]AGC
2251 GCTCAGC
BsaI 982 GAGACC GGTCTC(1/5)
2510 GAGACC
BsmBI 3624 CGTCTC CGTCTC(1/5)
243 GAGACG
BsoBI 977 CCCGAG C^[CT]CG[GA]G
1244 CCCGAG
Bsp1407I 2516 TGTACA T^GTACA
3068 TGTACA
BsrFI 985 ACCGGC [GA]^CCGG[CT]
1512 GCCGGT
BsrGI 2516 TGTACA T^GTACA
3068 TGTACA
BssSI 771 CACGAG CACGAG(-5/-1)
2607 CACGAG
CelII 1086 GCTGAGC GC^T[ACGT]AGC
2251 GCTCAGC
CfoI 309 GCGC GCG^C
2697 GCGC
Cfr10I 985 ACCGGC [GA]^CCGG[CT]
1512 GCCGGT
Eam1105I 249 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1196 GACCTCCTGTC
HhaI 309 GCGC GCG^C
2697 GCGC
HinP1I 309 GCGC G^CGC
2697 GCGC
HincII 1922 GTCAAC GT[CT]^[GA]AC
2766 GTCAAC
HindII 1922 GTCAAC GT[CT]^[GA]AC
2766 GTCAAC
MluNI 261 TGGCCA TGG^CCA
2760 TGGCCA
MscI 261 TGGCCA TGG^CCA
2760 TGGCCA
NcoI 2450 CCATGG C^CATGG
2757 CCATGG
SexAI 1334 ACCAGGT A^CC[AT]GGT
2388 ACCAGGT
SphI 1190 GCATGC GCATG^C
2000 GCATGC
SspBI 2516 TGTACA T^GTACA
3068 TGTACA
StuI 2076 AGGCCT AGG^CCT
2202 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII