##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflIII AocI ApoI AscI AseI
AsnI AviII AvrII BamHI BbsI
BclI BglII BlnI BpuAI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI MamI
MfeI MluI MroI MunI NheI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 1272 GGTACC G^GTACC
AflII 2612 CTTAAG C^TTAAG
AgeI 2166 ACCGGT A^CCGGT
AhdI 1578 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1885 AGCGCT AGC^GCT
ApaI 1902 GGGCCC GGGCC^C
Asp700I 1730 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1272 GGTACC G^GTACC
AspEI 1578 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1532 CACGTG CAC^GTG
BbvCI 2662 CCTCAGC CCTCAGC(-5/-2)
BciVI 3188 GTATCC GTATCC(6/5)
BfrI 2612 CTTAAG C^TTAAG
BsaAI 1532 CACGTG [CT]AC^GT[GA]
BsaI 1230 GAGACC GGTCTC(1/5)
BsmBI 2633 GAGACG CGTCTC(1/5)
BsmI 2956 GAATGC GAATGC(1/-1)
BstAPI 1135 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 743 GGTCACC G^GT[ACGT]ACC
BstPI 743 GGTCACC G^GT[ACGT]ACC
BstYI 2528 GGATCT [GA]^GATC[CT]
Eam1105I 1578 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1885 AGCGCT AGC^GCT
EcoNI 748 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 743 GGTCACC G^GT[ACGT]ACC
HincII 1096 GTTGAC GT[CT]^[GA]AC
HindII 1096 GTTGAC GT[CT]^[GA]AC
KpnI 1272 GGTACC GGTAC^C
KspI 50 CCGCGG CCGC^GG
MflI 2528 GGATCT [GA]^GATC[CT]
NdeI 1538 CATATG CA^TATG
NotI 126 GCGGCCGC GC^GGCCGC
PinAI 2166 ACCGGT A^CCGGT
PmaCI 1532 CACGTG CAC^GTG
PmlI 1532 CACGTG CAC^GTG
SacII 50 CCGCGG CCGC^GG
ScaI 988 AGTACT AGT^ACT
SexAI 2559 ACCTGGT A^CC[AT]GGT
StuI 521 AGGCCT AGG^CCT
XbaI 3427 TCTAGA T^CTAGA
XcmI 792 CCAAGGCCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2528 GGATCT [GA]^GATC[CT]
XmnI 1730 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 2765 GTGCAC G^TGCAC
3048 GTGCAC
AlwI 2146 GGATC GGATC(4/5)
2528 GGATC
ApaLI 2765 GTGCAC G^TGCAC
3048 GTGCAC
AspI 2489 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2813 GACATGGTC
BbeI 45 GGCGCC GGCGC^C
2996 GGCGCC
BcgI 2362 GCATGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2454 GCACTGTCATCG
BsaWI 1547 TCCGGT [AT]^CCGG[AT]
2166 ACCGGT
BsrBI 2746 CCGCTC CCGCTC(-3/-3)
124 GAGCGG
KasI 45 GGCGCC G^GCGCC
2996 GGCGCC
MaeII 1533 ACGT A^CGT
2544 ACGT
MseI 1971 TTAA T^TAA
2613 TTAA
NaeI 2377 GCCGGC GCC^GGC
2671 GCCGGC
NarI 45 GGCGCC GG^CGCC
2996 GGCGCC
NgoMIV 2377 GCCGGC G^CCGGC
2671 GCCGGC
NspI 358 GCATGT [GA]CATG^[CT]
2875 GCATGT
PflFI 2489 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2813 GACATGGTC
PshAI 1934 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2400 GACCACTGTC
SacI 22 GAGCTC GAGCT^C
718 GAGCTC
SbfI 455 CCTGCAGG CCTGCA^GG
524 CCTGCAGG
SfoI 45 GGCGCC GGC^GCC
2996 GGCGCC
SmlI 2612 CTTAAG C^T[CT][GA]AG
2966 CTCAAG
Sse8387I 455 CCTGCAGG CCTGCA^GG
524 CCTGCAGG
Tru9I 1971 TTAA T^TAA
2613 TTAA
Tsp509I 465 AATT ^AATT
1038 AATT
Tth111I 2489 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2813 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XmaCI XmaI