################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccIII AclI AhdI ApaI AscI Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BglI BlnI BsaI BseAI BsiEI BsiWI BspDI BspEI BssHII BstAPI BstBI BstEII BstPI ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I FseI FspI KasI KpnI KspI MluI MluNI MroI MscI MslI NarI NotI NruI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 91 GGCGTC G[GA]^CG[CT]C AflII 2385 CTTAAG C^TTAAG AgeI 1388 ACCGGT A^CCGGT Alw44I 3193 GTGCAC G^TGCAC Aor51HI 1188 AGCGCT AGC^GCT ApaLI 3193 GTGCAC G^TGCAC AseI 4538 ATTAAT AT^TAAT AsnI 4538 ATTAAT AT^TAAT AvaI 10 CCCGAG C^[CT]CG[GA]G BamHI 3047 GGATCC G^GATCC BanI 3524 GGTGCC G^G[CT][GA]CC BbsI 1080 GAAGAC GAAGAC(2/6) BclI 4693 TGATCA T^GATCA BcnI 1314 CCGGG CC^[GC]GG BfrI 2385 CTTAAG C^TTAAG BpuAI 1080 GAAGAC GAAGAC(2/6) BsaAI 4503 TACGTG [CT]AC^GT[GA] BsaHI 91 GGCGTC G[GA]^CG[CT]C BsmBI 2605 CGTCTC CGTCTC(1/5) BsmI 237 GAATGC GAATGC(1/-1) BsoBI 10 CCCGAG C^[CT]CG[GA]G BsrBI 3207 CCGCTC CCGCTC(-3/-3) BssSI 2762 CACGAG CACGAG(-5/-1) Bst1107I 3956 GTATAC GTA^TAC BstZ17I 3956 GTATAC GTA^TAC DraIII 1489 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2826 CGGCCA [CT]^GGCC[GA] Eco47III 1188 AGCGCT AGC^GCT EcoNI 1195 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 3909 ATGCAT ATGCA^T FauI 3206 CCCGC CCCGC(4/6) FbaI 4693 TGATCA T^GATCA Hin1I 91 GGCGTC G[GA]^CG[CT]C NaeI 56 GCCGGC GCC^GGC NciI 1314 CCGGG CC^[GC]GG NcoI 2637 CCATGG C^CATGG NdeI 3432 CATATG CA^TATG NgoMIV 56 GCCGGC G^CCGGC NheI 1185 GCTAGC G^CTAGC NsiI 3909 ATGCAT ATGCA^T PinAI 1388 ACCGGT A^CCGGT PshBI 4538 ATTAAT AT^TAAT PstI 4471 CTGCAG CTGCA^G SalI 2961 GTCGAC G^TCGAC ScaI 3356 AGTACT AGT^ACT SfcI 4471 CTGCAG C^T[GA][CT]AG SphI 3596 GCATGC GCATG^C StuI 84 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 2961 GTCGAC GT^[AC][GT]AC 3956 GTATAC AccII 1118 CGCG CG^CG 4457 CGCG AocI 20 CCTGAGG CC^T[ACGT]AGG 318 CCTAAGG BpmI 934 CTCCAG CTGGAG(16/14) 48 CTGGAG BsaWI 1074 TCCGGT [AT]^CCGG[AT] 1388 ACCGGT BsgI 2767 GTGCAG GTGCAG(16/14) 3730 GTGCAG Bsp1407I 1508 TGTACA T^GTACA 3300 TGTACA BspHI 977 TCATGA T^CATGA 1433 TCATGA BspMI 1150 ACCTGC ACCTGC(4/8) 4629 GCAGGT BsrFI 56 GCCGGC [GA]^CCGG[CT] 1388 ACCGGT BsrGI 1508 TGTACA T^GTACA 3300 TGTACA BstUI 1118 CGCG CG^CG 4457 CGCG BstXI 1998 CCATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3486 CCACATGACTGG Bsu36I 20 CCTGAGG CC^T[ACGT]AGG 318 CCTAAGG Cfr10I 56 GCCGGC [GA]^CCGG[CT] 1388 ACCGGT Eco81I 20 CCTGAGG CC^T[ACGT]AGG 318 CCTAAGG EcoRV 558 GATATC GAT^ATC 4762 GATATC EcoT14I 2637 CCATGG C^C[AT][AT]GG 3323 CCTTGG HaeII 31 AGCGCC [GA]GCGC^[CT] 1188 AGCGCT HpaI 176 GTTAAC GTT^AAC 3057 GTTAAC MfeI 1360 CAATTG C^AATTG 4199 CAATTG MunI 1360 CAATTG C^AATTG 4199 CAATTG MvnI 1118 CGCG CG^CG 4457 CGCG RcaI 977 TCATGA T^CATGA 1433 TCATGA SspBI 1508 TGTACA T^GTACA 3300 TGTACA StyI 2637 CCATGG C^C[AT][AT]GG 3323 CCTTGG XbaI 278 TCTAGA T^CTAGA 2330 TCTAGA XcmI 1216 CCAACAAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2226 CCAGAGGAGGAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanII BbvI BfaI BglII BlpI BmyI Bpu1102I BsaBI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DrdI DsaI EarI EcoO109I EcoRI EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI