##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
ApaI AscI Asp718I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BglI BlnI
BsaI BseAI BsiEI BsiWI BspDI
BspEI BssHII BstAPI BstBI BstEII
BstPI ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoO65I FseI FspI
KasI KpnI KspI MluI MluNI
MroI MscI MslI NarI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 91 GGCGTC G[GA]^CG[CT]C
AflII 2385 CTTAAG C^TTAAG
AgeI 1388 ACCGGT A^CCGGT
Alw44I 3193 GTGCAC G^TGCAC
Aor51HI 1188 AGCGCT AGC^GCT
ApaLI 3193 GTGCAC G^TGCAC
AseI 4538 ATTAAT AT^TAAT
AsnI 4538 ATTAAT AT^TAAT
AvaI 10 CCCGAG C^[CT]CG[GA]G
BamHI 3047 GGATCC G^GATCC
BanI 3524 GGTGCC G^G[CT][GA]CC
BbsI 1080 GAAGAC GAAGAC(2/6)
BclI 4693 TGATCA T^GATCA
BcnI 1314 CCGGG CC^[GC]GG
BfrI 2385 CTTAAG C^TTAAG
BpuAI 1080 GAAGAC GAAGAC(2/6)
BsaAI 4503 TACGTG [CT]AC^GT[GA]
BsaHI 91 GGCGTC G[GA]^CG[CT]C
BsmBI 2605 CGTCTC CGTCTC(1/5)
BsmI 237 GAATGC GAATGC(1/-1)
BsoBI 10 CCCGAG C^[CT]CG[GA]G
BsrBI 3207 CCGCTC CCGCTC(-3/-3)
BssSI 2762 CACGAG CACGAG(-5/-1)
Bst1107I 3956 GTATAC GTA^TAC
BstZ17I 3956 GTATAC GTA^TAC
DraIII 1489 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2826 CGGCCA [CT]^GGCC[GA]
Eco47III 1188 AGCGCT AGC^GCT
EcoNI 1195 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3909 ATGCAT ATGCA^T
FauI 3206 CCCGC CCCGC(4/6)
FbaI 4693 TGATCA T^GATCA
Hin1I 91 GGCGTC G[GA]^CG[CT]C
NaeI 56 GCCGGC GCC^GGC
NciI 1314 CCGGG CC^[GC]GG
NcoI 2637 CCATGG C^CATGG
NdeI 3432 CATATG CA^TATG
NgoMIV 56 GCCGGC G^CCGGC
NheI 1185 GCTAGC G^CTAGC
NsiI 3909 ATGCAT ATGCA^T
PinAI 1388 ACCGGT A^CCGGT
PshBI 4538 ATTAAT AT^TAAT
PstI 4471 CTGCAG CTGCA^G
SalI 2961 GTCGAC G^TCGAC
ScaI 3356 AGTACT AGT^ACT
SfcI 4471 CTGCAG C^T[GA][CT]AG
SphI 3596 GCATGC GCATG^C
StuI 84 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2961 GTCGAC GT^[AC][GT]AC
3956 GTATAC
AccII 1118 CGCG CG^CG
4457 CGCG
AocI 20 CCTGAGG CC^T[ACGT]AGG
318 CCTAAGG
BpmI 934 CTCCAG CTGGAG(16/14)
48 CTGGAG
BsaWI 1074 TCCGGT [AT]^CCGG[AT]
1388 ACCGGT
BsgI 2767 GTGCAG GTGCAG(16/14)
3730 GTGCAG
Bsp1407I 1508 TGTACA T^GTACA
3300 TGTACA
BspHI 977 TCATGA T^CATGA
1433 TCATGA
BspMI 1150 ACCTGC ACCTGC(4/8)
4629 GCAGGT
BsrFI 56 GCCGGC [GA]^CCGG[CT]
1388 ACCGGT
BsrGI 1508 TGTACA T^GTACA
3300 TGTACA
BstUI 1118 CGCG CG^CG
4457 CGCG
BstXI 1998 CCATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3486 CCACATGACTGG
Bsu36I 20 CCTGAGG CC^T[ACGT]AGG
318 CCTAAGG
Cfr10I 56 GCCGGC [GA]^CCGG[CT]
1388 ACCGGT
Eco81I 20 CCTGAGG CC^T[ACGT]AGG
318 CCTAAGG
EcoRV 558 GATATC GAT^ATC
4762 GATATC
EcoT14I 2637 CCATGG C^C[AT][AT]GG
3323 CCTTGG
HaeII 31 AGCGCC [GA]GCGC^[CT]
1188 AGCGCT
HpaI 176 GTTAAC GTT^AAC
3057 GTTAAC
MfeI 1360 CAATTG C^AATTG
4199 CAATTG
MunI 1360 CAATTG C^AATTG
4199 CAATTG
MvnI 1118 CGCG CG^CG
4457 CGCG
RcaI 977 TCATGA T^CATGA
1433 TCATGA
SspBI 1508 TGTACA T^GTACA
3300 TGTACA
StyI 2637 CCATGG C^C[AT][AT]GG
3323 CCTTGG
XbaI 278 TCTAGA T^CTAGA
2330 TCTAGA
XcmI 1216 CCAACAAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2226 CCAGAGGAGGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanII BbvI BfaI BglII
BlpI BmyI Bpu1102I BsaBI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EarI
EcoO109I EcoRI EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI