##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AgeI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       BamHI       BbvCI       BciVI       BclI        
BsaBI       BseAI       BsiWI       BsmI        BspDI       
BspEI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     Cfr10I      ClaI        CpoI        
DraIII      EcoNI       EcoRI       EcoRV       FbaI        
FseI        FspI        HincII      HindII      HpaI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NdeI        NgoMIV      NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SalI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       Sse8387I    
SwaI        Tth111I     XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AflII        3109  CTTAAG               C^TTAAG
AocI          856  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       341  AGCGCT               AGC^GCT
AvrII        3247  CCTAGG               C^CTAGG
BalI         2565  TGGCCA               TGG^CCA
BbeI          242  GGCGCC               GGCGC^C
BcgI         2793  GCAGAGGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         3109  CTTAAG               C^TTAAG
BlnI         3247  CCTAGG               C^CTAGG
BlpI         2822  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2822  GCTCAGC              GC^T[ACGT]AGC
BsaI         2587  GGTCTC               GGTCTC(1/5)
BsaWI        1478  ACCGGA               [AT]^CCGG[AT]
BsiEI         202  CGGCCG               CG[GA][CT]^CG
BsmBI          74  GAGACG               CGTCTC(1/5)
Bsp1407I     3097  TGTACA               T^GTACA
BspLU11I     2751  ACATGT               A^CATGT
BsrDI        1241  CATTGC               GCAATG(2/0)
BsrGI        3097  TGTACA               T^GTACA
BstAPI       1674  GCAGTCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2558  GGTGACC              G^GT[ACGT]ACC
BstF5I       3094  GGATG                GGATG(2/0)
BstPI        2558  GGTGACC              G^GT[ACGT]ACC
Bsu36I        856  CCTCAGG              CC^T[ACGT]AGG
CelII        2822  GCTCAGC              GC^T[ACGT]AGC
DraI         1991  TTTAAA               TTT^AAA
DrdI         1230  GACGTGGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          202  CGGCCG               C^GGCCG
EclXI         202  CGGCCG               C^GGCCG
Eco47III      341  AGCGCT               AGC^GCT
Eco52I        202  CGGCCG               C^GGCCG
Eco81I        856  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      2558  GGTGACC              G^GT[ACGT]ACC
EcoT22I      2385  ATGCAT               ATGCA^T
FokI         3094  GGATG                GGATG(9/13)
KasI          242  GGCGCC               G^GCGCC
MluNI        2565  TGGCCA               TGG^CCA
MscI         2565  TGGCCA               TGG^CCA
NarI          242  GGCGCC               GG^CGCC
NheI          395  GCTAGC               G^CTAGC
NsiI         2385  ATGCAT               ATGCA^T
PaeR7I       2801  CTCGAG               C^TCGAG
ScaI         2211  AGTACT               AGT^ACT
SfoI          242  GGCGCC               GGC^GCC
SpeI         2461  ACTAGT               A^CTAGT
SphI         1138  GCATGC               GCATG^C
SspBI        3097  TGTACA               T^GTACA
SspI         3331  AATATT               AAT^ATT
XbaI         2467  TCTAGA               T^CTAGA
XcmI         2529  CCAATGTGTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2801  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI         1425  AAATTT               [GA]^AATT[CT]
             2265  AAATTC              
AhdI           76  GACGAACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2957  GACCTTGGGTC         
ApoI         1425  AAATTT               [GA]^AATT[CT]
             2265  AAATTC              
AspEI          76  GACGAACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2957  GACCTTGGGTC         
BglII        2857  AGATCT               A^GATCT
             3025  AGATCT              
BspHI         794  TCATGA               T^CATGA
             1270  TCATGA              
BsrBI           4  CCGCTC               CCGCTC(-3/-3)
              870  GAGCGG              
BstXI         320  CCAGTCCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2352  CCAGAGCTGTGG        
Eam1105I       76  GACGAACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2957  GACCTTGGGTC         
HindIII      2313  AAGCTT               A^AGCTT
             2676  AAGCTT              
KspI          235  CCGCGG               CCGC^GG
              267  CCGCGG              
PflMI        2270  CCATAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2993  CCATTTCTTGG         
RcaI          794  TCATGA               T^CATGA
             1270  TCATGA              
SacII         235  CCGCGG               CCGC^GG
              267  CCGCGG              
SmaI          113  CCCGGG               CCC^GGG
              246  CCCGGG              
Van91I       2270  CCATAGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2993  CCATTTCTTGG         
XmaCI         113  CCCGGG               C^CCGGG
              246  CCCGGG              
XmaI          113  CCCGGG               C^CCGGG
              246  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AspHI       AvaI        
AvaII       BanI        BanII       BbrPI       BbsI        
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PmaCI       PmlI        PpuMI       
PstI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII