################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII BamHI BbvCI BciVI BclI BsaBI BseAI BsiWI BsmI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraIII EcoNI EcoRI EcoRV FbaI FseI FspI HincII HindII HpaI KpnI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AflII 3109 CTTAAG C^TTAAG AocI 856 CCTCAGG CC^T[ACGT]AGG Aor51HI 341 AGCGCT AGC^GCT AvrII 3247 CCTAGG C^CTAGG BalI 2565 TGGCCA TGG^CCA BbeI 242 GGCGCC GGCGC^C BcgI 2793 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3109 CTTAAG C^TTAAG BlnI 3247 CCTAGG C^CTAGG BlpI 2822 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2822 GCTCAGC GC^T[ACGT]AGC BsaI 2587 GGTCTC GGTCTC(1/5) BsaWI 1478 ACCGGA [AT]^CCGG[AT] BsiEI 202 CGGCCG CG[GA][CT]^CG BsmBI 74 GAGACG CGTCTC(1/5) Bsp1407I 3097 TGTACA T^GTACA BspLU11I 2751 ACATGT A^CATGT BsrDI 1241 CATTGC GCAATG(2/0) BsrGI 3097 TGTACA T^GTACA BstAPI 1674 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2558 GGTGACC G^GT[ACGT]ACC BstF5I 3094 GGATG GGATG(2/0) BstPI 2558 GGTGACC G^GT[ACGT]ACC Bsu36I 856 CCTCAGG CC^T[ACGT]AGG CelII 2822 GCTCAGC GC^T[ACGT]AGC DraI 1991 TTTAAA TTT^AAA DrdI 1230 GACGTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 202 CGGCCG C^GGCCG EclXI 202 CGGCCG C^GGCCG Eco47III 341 AGCGCT AGC^GCT Eco52I 202 CGGCCG C^GGCCG Eco81I 856 CCTCAGG CC^T[ACGT]AGG EcoO65I 2558 GGTGACC G^GT[ACGT]ACC EcoT22I 2385 ATGCAT ATGCA^T FokI 3094 GGATG GGATG(9/13) KasI 242 GGCGCC G^GCGCC MluNI 2565 TGGCCA TGG^CCA MscI 2565 TGGCCA TGG^CCA NarI 242 GGCGCC GG^CGCC NheI 395 GCTAGC G^CTAGC NsiI 2385 ATGCAT ATGCA^T PaeR7I 2801 CTCGAG C^TCGAG ScaI 2211 AGTACT AGT^ACT SfoI 242 GGCGCC GGC^GCC SpeI 2461 ACTAGT A^CTAGT SphI 1138 GCATGC GCATG^C SspBI 3097 TGTACA T^GTACA SspI 3331 AATATT AAT^ATT XbaI 2467 TCTAGA T^CTAGA XcmI 2529 CCAATGTGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2801 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 1425 AAATTT [GA]^AATT[CT] 2265 AAATTC AhdI 76 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2957 GACCTTGGGTC ApoI 1425 AAATTT [GA]^AATT[CT] 2265 AAATTC AspEI 76 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2957 GACCTTGGGTC BglII 2857 AGATCT A^GATCT 3025 AGATCT BspHI 794 TCATGA T^CATGA 1270 TCATGA BsrBI 4 CCGCTC CCGCTC(-3/-3) 870 GAGCGG BstXI 320 CCAGTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2352 CCAGAGCTGTGG Eam1105I 76 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2957 GACCTTGGGTC HindIII 2313 AAGCTT A^AGCTT 2676 AAGCTT KspI 235 CCGCGG CCGC^GG 267 CCGCGG PflMI 2270 CCATAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2993 CCATTTCTTGG RcaI 794 TCATGA T^CATGA 1270 TCATGA SacII 235 CCGCGG CCGC^GG 267 CCGCGG SmaI 113 CCCGGG CCC^GGG 246 CCCGGG Van91I 2270 CCATAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2993 CCATTTCTTGG XmaCI 113 CCCGGG C^CCGGG 246 CCCGGG XmaI 113 CCCGGG C^CCGGG 246 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbrPI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII