##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII BamHI BbvCI BciVI BclI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BsrFI BssHII BssSI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraIII EcoNI EcoRI EcoRV FbaI
FseI FspI HincII HindII HpaI
KpnI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NotI
NruI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AflII 3109 CTTAAG C^TTAAG
AocI 856 CCTCAGG CC^T[ACGT]AGG
Aor51HI 341 AGCGCT AGC^GCT
AvrII 3247 CCTAGG C^CTAGG
BalI 2565 TGGCCA TGG^CCA
BbeI 242 GGCGCC GGCGC^C
BcgI 2793 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3109 CTTAAG C^TTAAG
BlnI 3247 CCTAGG C^CTAGG
BlpI 2822 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2822 GCTCAGC GC^T[ACGT]AGC
BsaI 2587 GGTCTC GGTCTC(1/5)
BsaWI 1478 ACCGGA [AT]^CCGG[AT]
BsiEI 202 CGGCCG CG[GA][CT]^CG
BsmBI 74 GAGACG CGTCTC(1/5)
Bsp1407I 3097 TGTACA T^GTACA
BspLU11I 2751 ACATGT A^CATGT
BsrDI 1241 CATTGC GCAATG(2/0)
BsrGI 3097 TGTACA T^GTACA
BstAPI 1674 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2558 GGTGACC G^GT[ACGT]ACC
BstF5I 3094 GGATG GGATG(2/0)
BstPI 2558 GGTGACC G^GT[ACGT]ACC
Bsu36I 856 CCTCAGG CC^T[ACGT]AGG
CelII 2822 GCTCAGC GC^T[ACGT]AGC
DraI 1991 TTTAAA TTT^AAA
DrdI 1230 GACGTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 202 CGGCCG C^GGCCG
EclXI 202 CGGCCG C^GGCCG
Eco47III 341 AGCGCT AGC^GCT
Eco52I 202 CGGCCG C^GGCCG
Eco81I 856 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2558 GGTGACC G^GT[ACGT]ACC
EcoT22I 2385 ATGCAT ATGCA^T
FokI 3094 GGATG GGATG(9/13)
KasI 242 GGCGCC G^GCGCC
MluNI 2565 TGGCCA TGG^CCA
MscI 2565 TGGCCA TGG^CCA
NarI 242 GGCGCC GG^CGCC
NheI 395 GCTAGC G^CTAGC
NsiI 2385 ATGCAT ATGCA^T
PaeR7I 2801 CTCGAG C^TCGAG
ScaI 2211 AGTACT AGT^ACT
SfoI 242 GGCGCC GGC^GCC
SpeI 2461 ACTAGT A^CTAGT
SphI 1138 GCATGC GCATG^C
SspBI 3097 TGTACA T^GTACA
SspI 3331 AATATT AAT^ATT
XbaI 2467 TCTAGA T^CTAGA
XcmI 2529 CCAATGTGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2801 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 1425 AAATTT [GA]^AATT[CT]
2265 AAATTC
AhdI 76 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2957 GACCTTGGGTC
ApoI 1425 AAATTT [GA]^AATT[CT]
2265 AAATTC
AspEI 76 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2957 GACCTTGGGTC
BglII 2857 AGATCT A^GATCT
3025 AGATCT
BspHI 794 TCATGA T^CATGA
1270 TCATGA
BsrBI 4 CCGCTC CCGCTC(-3/-3)
870 GAGCGG
BstXI 320 CCAGTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2352 CCAGAGCTGTGG
Eam1105I 76 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2957 GACCTTGGGTC
HindIII 2313 AAGCTT A^AGCTT
2676 AAGCTT
KspI 235 CCGCGG CCGC^GG
267 CCGCGG
PflMI 2270 CCATAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2993 CCATTTCTTGG
RcaI 794 TCATGA T^CATGA
1270 TCATGA
SacII 235 CCGCGG CCGC^GG
267 CCGCGG
SmaI 113 CCCGGG CCC^GGG
246 CCCGGG
Van91I 2270 CCATAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2993 CCATTTCTTGG
XmaCI 113 CCCGGG C^CCGGG
246 CCCGGG
XmaI 113 CCCGGG C^CCGGG
246 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BanII BbrPI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PpuMI
PstI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII