##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AflII       
AgeI        AhdI        Aor51HI     ApaI        AscI        
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BcgI        BfrI        BlnI        BsaAI       
BsaHI       BsaWI       BseAI       BsiWI       BsmBI       
BsmI        BspDI       BspEI       BsrBI       BsrDI       
BssHII      BstBI       BstEII      BstPI       BstUI       
ClaI        CpoI        DrdI        Eam1105I    Eco47III    
EcoNI       EcoO65I     EcoRV       EcoT22I     FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KspI        MfeI        MluI        
MroI        MunI        MvnI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AclI         2795  AACGTT               AA^CGTT
AseI         3333  ATTAAT               AT^TAAT
AsnI         3333  ATTAAT               AT^TAAT
Asp700I      2607  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1576  CCCGAG               C^[CT]CG[GA]G
BamHI        2835  GGATCC               G^GATCC
BbvCI        4095  GCTGAGG              CCTCAGC(-5/-2)
BglII        3287  AGATCT               A^GATCT
BlpI         4427  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     4427  GCTAAGC              GC^T[ACGT]AGC
BsaBI        1561  GATTCCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        2067  CGGCCG               CG[GA][CT]^CG
BsoBI        1576  CCCGAG               C^[CT]CG[GA]G
BspHI        3038  TCATGA               T^CATGA
BsrFI        2069  GCCGGC               [GA]^CCGG[CT]
BssSI        4084  CTCGTG               CACGAG(-5/-1)
Bst1107I     4198  GTATAC               GTA^TAC
BstAPI       1276  GCAAATTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        4232  CCAAAAAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      4198  GTATAC               GTA^TAC
CelII        4427  GCTAAGC              GC^T[ACGT]AGC
CfoI         2190  GCGC                 GCG^C
Cfr10I       2069  GCCGGC               [GA]^CCGG[CT]
DraII         982  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII       1182  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2067  CGGCCG               C^GGCCG
EclXI        2067  CGGCCG               C^GGCCG
Eco52I       2067  CGGCCG               C^GGCCG
EcoO109I      982  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        2869  GAATTC               G^AATTC
HaeII        2189  AGCGCC               [GA]GCGC^[CT]
HhaI         2190  GCGC                 GCG^C
HinP1I       2190  GCGC                 G^CGC
HindIII      4351  AAGCTT               A^AGCTT
MamI         1561  GATTCCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         2069  GCCGGC               GCC^GGC
NgoMIV       2069  GCCGGC               G^CCGGC
PpuMI         982  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        3333  ATTAAT               AT^TAAT
Psp1406I     2795  AACGTT               AA^CGTT
RcaI         3038  TCATGA               T^CATGA
SapI         3532  GAAGAGC              GCTCTTC(1/4)
SspI         1461  AATATT               AAT^ATT
SwaI         3380  ATTTAAAT             ATTT^AAAT
XmnI         2607  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Acc65I        623  GGTACC               G^GTACC
             1817  GGTACC              
Alw44I        915  GTGCAC               G^TGCAC
             4185  GTGCAC              
AocI         2547  CCTGAGG              CC^T[ACGT]AGG
             3948  CCTCAGG             
ApaLI         915  GTGCAC               G^TGCAC
             4185  GTGCAC              
Asp718I       623  GGTACC               G^GTACC
             1817  GGTACC              
BciVI        2965  GGATAC               GTATCC(6/5)
              369  GTATCC              
BglI         1775  GCCATCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2459  GCCACAGTGGC         
BseRI        2000  CTCCTC               GAGGAG(10/8)
             4062  GAGGAG              
Bsp1407I     1329  TGTACA               T^GTACA
             4307  TGTACA              
BsrGI        1329  TGTACA               T^GTACA
             4307  TGTACA              
Bsu36I       2547  CCTGAGG              CC^T[ACGT]AGG
             3948  CCTCAGG             
Eco81I       2547  CCTGAGG              CC^T[ACGT]AGG
             3948  CCTCAGG             
KpnI          623  GGTACC               GGTAC^C
             1817  GGTACC              
MspA1I       1770  CAGCTG               C[AC]G^C[GT]G
             2028  CAGCTG              
PflMI        1257  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1868  CCAGCCTGTGG         
PvuII        1770  CAGCTG               CAG^CTG
             2028  CAGCTG              
SpeI           91  ACTAGT               A^CTAGT
             1314  ACTAGT              
SspBI        1329  TGTACA               T^GTACA
             4307  TGTACA              
TfiI         1561  GATTC                G^A[AT]TC
             2945  GAATC               
Van91I       1257  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1868  CCAGCCTGTGG         
XcmI          667  CCATCTTACGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              876  CCACAGTCTGAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BclI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspLU11I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DsaI        EaeI        
EarI        EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII