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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI AhdI Aor51HI ApaI AscI
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BfrI BlnI BsaAI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmI BspDI BspEI BsrBI BsrDI
BssHII BstBI BstEII BstPI BstUI
ClaI CpoI DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KspI MfeI MluI
MroI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AclI 2795 AACGTT AA^CGTT
AseI 3333 ATTAAT AT^TAAT
AsnI 3333 ATTAAT AT^TAAT
Asp700I 2607 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1576 CCCGAG C^[CT]CG[GA]G
BamHI 2835 GGATCC G^GATCC
BbvCI 4095 GCTGAGG CCTCAGC(-5/-2)
BglII 3287 AGATCT A^GATCT
BlpI 4427 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4427 GCTAAGC GC^T[ACGT]AGC
BsaBI 1561 GATTCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2067 CGGCCG CG[GA][CT]^CG
BsoBI 1576 CCCGAG C^[CT]CG[GA]G
BspHI 3038 TCATGA T^CATGA
BsrFI 2069 GCCGGC [GA]^CCGG[CT]
BssSI 4084 CTCGTG CACGAG(-5/-1)
Bst1107I 4198 GTATAC GTA^TAC
BstAPI 1276 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 4232 CCAAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4198 GTATAC GTA^TAC
CelII 4427 GCTAAGC GC^T[ACGT]AGC
CfoI 2190 GCGC GCG^C
Cfr10I 2069 GCCGGC [GA]^CCGG[CT]
DraII 982 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1182 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2067 CGGCCG C^GGCCG
EclXI 2067 CGGCCG C^GGCCG
Eco52I 2067 CGGCCG C^GGCCG
EcoO109I 982 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 2869 GAATTC G^AATTC
HaeII 2189 AGCGCC [GA]GCGC^[CT]
HhaI 2190 GCGC GCG^C
HinP1I 2190 GCGC G^CGC
HindIII 4351 AAGCTT A^AGCTT
MamI 1561 GATTCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2069 GCCGGC GCC^GGC
NgoMIV 2069 GCCGGC G^CCGGC
PpuMI 982 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 3333 ATTAAT AT^TAAT
Psp1406I 2795 AACGTT AA^CGTT
RcaI 3038 TCATGA T^CATGA
SapI 3532 GAAGAGC GCTCTTC(1/4)
SspI 1461 AATATT AAT^ATT
SwaI 3380 ATTTAAAT ATTT^AAAT
XmnI 2607 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 623 GGTACC G^GTACC
1817 GGTACC
Alw44I 915 GTGCAC G^TGCAC
4185 GTGCAC
AocI 2547 CCTGAGG CC^T[ACGT]AGG
3948 CCTCAGG
ApaLI 915 GTGCAC G^TGCAC
4185 GTGCAC
Asp718I 623 GGTACC G^GTACC
1817 GGTACC
BciVI 2965 GGATAC GTATCC(6/5)
369 GTATCC
BglI 1775 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2459 GCCACAGTGGC
BseRI 2000 CTCCTC GAGGAG(10/8)
4062 GAGGAG
Bsp1407I 1329 TGTACA T^GTACA
4307 TGTACA
BsrGI 1329 TGTACA T^GTACA
4307 TGTACA
Bsu36I 2547 CCTGAGG CC^T[ACGT]AGG
3948 CCTCAGG
Eco81I 2547 CCTGAGG CC^T[ACGT]AGG
3948 CCTCAGG
KpnI 623 GGTACC GGTAC^C
1817 GGTACC
MspA1I 1770 CAGCTG C[AC]G^C[GT]G
2028 CAGCTG
PflMI 1257 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1868 CCAGCCTGTGG
PvuII 1770 CAGCTG CAG^CTG
2028 CAGCTG
SpeI 91 ACTAGT A^CTAGT
1314 ACTAGT
SspBI 1329 TGTACA T^GTACA
4307 TGTACA
TfiI 1561 GATTC G^A[AT]TC
2945 GAATC
Van91I 1257 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1868 CCAGCCTGTGG
XcmI 667 CCATCTTACGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
876 CCACAGTCTGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII