################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII AccII AccIII AcyI AflII AgeI AhdI Aor51HI ApaI AscI AspEI AspI AviII AvrII BbeI BbrPI BcgI BfrI BlnI BsaAI BsaHI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BsrBI BsrDI BssHII BstBI BstEII BstPI BstUI ClaI CpoI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRV EcoT22I FseI FspI HgaI Hin1I HincII HindII HpaI KasI KspI MfeI MluI MroI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AclI 2795 AACGTT AA^CGTT AseI 3333 ATTAAT AT^TAAT AsnI 3333 ATTAAT AT^TAAT Asp700I 2607 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1576 CCCGAG C^[CT]CG[GA]G BamHI 2835 GGATCC G^GATCC BbvCI 4095 GCTGAGG CCTCAGC(-5/-2) BglII 3287 AGATCT A^GATCT BlpI 4427 GCTAAGC GC^T[ACGT]AGC Bpu1102I 4427 GCTAAGC GC^T[ACGT]AGC BsaBI 1561 GATTCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 2067 CGGCCG CG[GA][CT]^CG BsoBI 1576 CCCGAG C^[CT]CG[GA]G BspHI 3038 TCATGA T^CATGA BsrFI 2069 GCCGGC [GA]^CCGG[CT] BssSI 4084 CTCGTG CACGAG(-5/-1) Bst1107I 4198 GTATAC GTA^TAC BstAPI 1276 GCAAATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 4232 CCAAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 4198 GTATAC GTA^TAC CelII 4427 GCTAAGC GC^T[ACGT]AGC CfoI 2190 GCGC GCG^C Cfr10I 2069 GCCGGC [GA]^CCGG[CT] DraII 982 GGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 1182 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2067 CGGCCG C^GGCCG EclXI 2067 CGGCCG C^GGCCG Eco52I 2067 CGGCCG C^GGCCG EcoO109I 982 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 2869 GAATTC G^AATTC HaeII 2189 AGCGCC [GA]GCGC^[CT] HhaI 2190 GCGC GCG^C HinP1I 2190 GCGC G^CGC HindIII 4351 AAGCTT A^AGCTT MamI 1561 GATTCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2069 GCCGGC GCC^GGC NgoMIV 2069 GCCGGC G^CCGGC PpuMI 982 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 3333 ATTAAT AT^TAAT Psp1406I 2795 AACGTT AA^CGTT RcaI 3038 TCATGA T^CATGA SapI 3532 GAAGAGC GCTCTTC(1/4) SspI 1461 AATATT AAT^ATT SwaI 3380 ATTTAAAT ATTT^AAAT XmnI 2607 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 623 GGTACC G^GTACC 1817 GGTACC Alw44I 915 GTGCAC G^TGCAC 4185 GTGCAC AocI 2547 CCTGAGG CC^T[ACGT]AGG 3948 CCTCAGG ApaLI 915 GTGCAC G^TGCAC 4185 GTGCAC Asp718I 623 GGTACC G^GTACC 1817 GGTACC BciVI 2965 GGATAC GTATCC(6/5) 369 GTATCC BglI 1775 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2459 GCCACAGTGGC BseRI 2000 CTCCTC GAGGAG(10/8) 4062 GAGGAG Bsp1407I 1329 TGTACA T^GTACA 4307 TGTACA BsrGI 1329 TGTACA T^GTACA 4307 TGTACA Bsu36I 2547 CCTGAGG CC^T[ACGT]AGG 3948 CCTCAGG Eco81I 2547 CCTGAGG CC^T[ACGT]AGG 3948 CCTCAGG KpnI 623 GGTACC GGTAC^C 1817 GGTACC MspA1I 1770 CAGCTG C[AC]G^C[GT]G 2028 CAGCTG PflMI 1257 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1868 CCAGCCTGTGG PvuII 1770 CAGCTG CAG^CTG 2028 CAGCTG SpeI 91 ACTAGT A^CTAGT 1314 ACTAGT SspBI 1329 TGTACA T^GTACA 4307 TGTACA TfiI 1561 GATTC G^A[AT]TC 2945 GAATC Van91I 1257 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1868 CCAGCCTGTGG XcmI 667 CCATCTTACGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 876 CCACAGTCTGAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII