################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AviII BamHI BbrPI BcgI BclI BglII BsaBI BsaI BspDI BspLU11I BsrFI BssHII BstEII BstPI Cfr10I ClaI CpoI Eam1105I EcoO65I EcoRI EcoRV FbaI FseI FspI HpaI KpnI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI ScaI SexAI SfiI SgrAI SmaI SpeI SphI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 910 TCCGGA T^CCGGA AflII 3770 CTTAAG C^TTAAG AflIII 2400 ACGTGT A^C[GA][CT]GT Aor51HI 2085 AGCGCT AGC^GCT AspI 1532 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 572 CCCGAG C^[CT]CG[GA]G BbeI 205 GGCGCC GGCGC^C BciVI 1558 GTATCC GTATCC(6/5) BfrI 3770 CTTAAG C^TTAAG BglI 30 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2081 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2081 GCTCAGC GC^T[ACGT]AGC BsaAI 230 TACGTA [CT]AC^GT[GA] BsaWI 910 TCCGGA [AT]^CCGG[AT] BseAI 910 TCCGGA T^CCGGA BsoBI 572 CCCGAG C^[CT]CG[GA]G BspEI 910 TCCGGA T^CCGGA BspHI 4074 TCATGA T^CATGA BspMI 600 ACCTGC ACCTGC(4/8) BsrBI 809 GAGCGG CCGCTC(-3/-3) BsrDI 590 CATTGC GCAATG(2/0) BssSI 386 CTCGTG CACGAG(-5/-1) BstBI 3250 TTCGAA TT^CGAA CelII 2081 GCTCAGC GC^T[ACGT]AGC EagI 61 CGGCCG C^GGCCG EclXI 61 CGGCCG C^GGCCG Eco47III 2085 AGCGCT AGC^GCT Eco52I 61 CGGCCG C^GGCCG HincII 3700 GTTGAC GT[CT]^[GA]AC HindII 3700 GTTGAC GT[CT]^[GA]AC HindIII 2340 AAGCTT A^AGCTT KasI 205 GGCGCC G^GCGCC KspI 34 CCGCGG CCGC^GG MroI 910 TCCGGA T^CCGGA NarI 205 GGCGCC GG^CGCC NspV 3250 TTCGAA TT^CGAA PflFI 1532 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2466 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4074 TCATGA T^CATGA SacII 34 CCGCGG CCGC^GG SapI 1042 GAAGAGC GCTCTTC(1/4) SbfI 1132 CCTGCAGG CCTGCA^GG SfoI 205 GGCGCC GGC^GCC SfuI 3250 TTCGAA TT^CGAA SnaBI 230 TACGTA TAC^GTA Sse8387I 1132 CCTGCAGG CCTGCA^GG SspI 4110 AATATT AAT^ATT Tth111I 1532 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AocI 239 CCTGAGG CC^T[ACGT]AGG 2415 CCTCAGG BsiEI 61 CGGCCG CG[GA][CT]^CG 3275 CGGTCG BsiWI 228 CGTACG C^GTACG 232 CGTACG BsmI 1295 GAATGC GAATGC(1/-1) 1339 GAATGC Bsp1407I 2816 TGTACA T^GTACA 4224 TGTACA BsrGI 2816 TGTACA T^GTACA 4224 TGTACA Bst1107I 945 GTATAC GTA^TAC 2656 GTATAC BstAPI 1543 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2176 GCAGAGCTTGC BstXI 2546 CCACAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3138 CCACACATTTGG BstYI 1140 AGATCC [GA]^GATC[CT] 3361 GGATCT BstZ17I 945 GTATAC GTA^TAC 2656 GTATAC Bsu36I 239 CCTGAGG CC^T[ACGT]AGG 2415 CCTCAGG DraI 4059 TTTAAA TTT^AAA 4126 TTTAAA DraIII 500 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1720 CACCGTGTG DrdI 1594 GACTGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3988 GACTCTACAGTC Eco81I 239 CCTGAGG CC^T[ACGT]AGG 2415 CCTCAGG EcoNI 2614 CCTGTCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3408 CCTTTGTAAGG EcoT22I 2588 ATGCAT ATGCA^T 3007 ATGCAT HaeII 205 GGCGCC [GA]GCGC^[CT] 2085 AGCGCT MflI 1140 AGATCC [GA]^GATC[CT] 3361 GGATCT NcoI 940 CCATGG C^CATGG 2142 CCATGG NsiI 2588 ATGCAT ATGCA^T 3007 ATGCAT PflMI 2113 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2328 CCAGTGGCTGG PstI 1133 CTGCAG CTGCA^G 3182 CTGCAG SacI 2690 GAGCTC GAGCT^C 3109 GAGCTC SspBI 2816 TGTACA T^GTACA 4224 TGTACA Van91I 2113 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2328 CCAGTGGCTGG XhoII 1140 AGATCC [GA]^GATC[CT] 3361 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmnI