##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
ApaI AscI AseI AsnI Asp718I
AspEI AviII BamHI BbrPI BcgI
BclI BglII BsaBI BsaI BspDI
BspLU11I BsrFI BssHII BstEII BstPI
Cfr10I ClaI CpoI Eam1105I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HpaI KpnI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfiI SgrAI SmaI SpeI
SphI StuI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 910 TCCGGA T^CCGGA
AflII 3770 CTTAAG C^TTAAG
AflIII 2400 ACGTGT A^C[GA][CT]GT
Aor51HI 2085 AGCGCT AGC^GCT
AspI 1532 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 572 CCCGAG C^[CT]CG[GA]G
BbeI 205 GGCGCC GGCGC^C
BciVI 1558 GTATCC GTATCC(6/5)
BfrI 3770 CTTAAG C^TTAAG
BglI 30 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2081 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2081 GCTCAGC GC^T[ACGT]AGC
BsaAI 230 TACGTA [CT]AC^GT[GA]
BsaWI 910 TCCGGA [AT]^CCGG[AT]
BseAI 910 TCCGGA T^CCGGA
BsoBI 572 CCCGAG C^[CT]CG[GA]G
BspEI 910 TCCGGA T^CCGGA
BspHI 4074 TCATGA T^CATGA
BspMI 600 ACCTGC ACCTGC(4/8)
BsrBI 809 GAGCGG CCGCTC(-3/-3)
BsrDI 590 CATTGC GCAATG(2/0)
BssSI 386 CTCGTG CACGAG(-5/-1)
BstBI 3250 TTCGAA TT^CGAA
CelII 2081 GCTCAGC GC^T[ACGT]AGC
EagI 61 CGGCCG C^GGCCG
EclXI 61 CGGCCG C^GGCCG
Eco47III 2085 AGCGCT AGC^GCT
Eco52I 61 CGGCCG C^GGCCG
HincII 3700 GTTGAC GT[CT]^[GA]AC
HindII 3700 GTTGAC GT[CT]^[GA]AC
HindIII 2340 AAGCTT A^AGCTT
KasI 205 GGCGCC G^GCGCC
KspI 34 CCGCGG CCGC^GG
MroI 910 TCCGGA T^CCGGA
NarI 205 GGCGCC GG^CGCC
NspV 3250 TTCGAA TT^CGAA
PflFI 1532 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2466 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4074 TCATGA T^CATGA
SacII 34 CCGCGG CCGC^GG
SapI 1042 GAAGAGC GCTCTTC(1/4)
SbfI 1132 CCTGCAGG CCTGCA^GG
SfoI 205 GGCGCC GGC^GCC
SfuI 3250 TTCGAA TT^CGAA
SnaBI 230 TACGTA TAC^GTA
Sse8387I 1132 CCTGCAGG CCTGCA^GG
SspI 4110 AATATT AAT^ATT
Tth111I 1532 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AocI 239 CCTGAGG CC^T[ACGT]AGG
2415 CCTCAGG
BsiEI 61 CGGCCG CG[GA][CT]^CG
3275 CGGTCG
BsiWI 228 CGTACG C^GTACG
232 CGTACG
BsmI 1295 GAATGC GAATGC(1/-1)
1339 GAATGC
Bsp1407I 2816 TGTACA T^GTACA
4224 TGTACA
BsrGI 2816 TGTACA T^GTACA
4224 TGTACA
Bst1107I 945 GTATAC GTA^TAC
2656 GTATAC
BstAPI 1543 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2176 GCAGAGCTTGC
BstXI 2546 CCACAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3138 CCACACATTTGG
BstYI 1140 AGATCC [GA]^GATC[CT]
3361 GGATCT
BstZ17I 945 GTATAC GTA^TAC
2656 GTATAC
Bsu36I 239 CCTGAGG CC^T[ACGT]AGG
2415 CCTCAGG
DraI 4059 TTTAAA TTT^AAA
4126 TTTAAA
DraIII 500 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1720 CACCGTGTG
DrdI 1594 GACTGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3988 GACTCTACAGTC
Eco81I 239 CCTGAGG CC^T[ACGT]AGG
2415 CCTCAGG
EcoNI 2614 CCTGTCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3408 CCTTTGTAAGG
EcoT22I 2588 ATGCAT ATGCA^T
3007 ATGCAT
HaeII 205 GGCGCC [GA]GCGC^[CT]
2085 AGCGCT
MflI 1140 AGATCC [GA]^GATC[CT]
3361 GGATCT
NcoI 940 CCATGG C^CATGG
2142 CCATGG
NsiI 2588 ATGCAT ATGCA^T
3007 ATGCAT
PflMI 2113 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2328 CCAGTGGCTGG
PstI 1133 CTGCAG CTGCA^G
3182 CTGCAG
SacI 2690 GAGCTC GAGCT^C
3109 GAGCTC
SspBI 2816 TGTACA T^GTACA
4224 TGTACA
Van91I 2113 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2328 CCAGTGGCTGG
XhoII 1140 AGATCC [GA]^GATC[CT]
3361 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaLI ApoI Asp700I AspHI AvaII
AvrII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmnI