##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       BamHI       BbrPI       BcgI        
BclI        BglII       BsaBI       BsaI        BspDI       
BspLU11I    BsrFI       BssHII      BstEII      BstPI       
Cfr10I      ClaI        CpoI        Eam1105I    EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HpaI        KpnI        MamI        MfeI        MluI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NspI        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SalI        ScaI        
SexAI       SfiI        SgrAI       SmaI        SpeI        
SphI        StuI        SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII        910  TCCGGA               T^CCGGA
AflII        3770  CTTAAG               C^TTAAG
AflIII       2400  ACGTGT               A^C[GA][CT]GT
Aor51HI      2085  AGCGCT               AGC^GCT
AspI         1532  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          572  CCCGAG               C^[CT]CG[GA]G
BbeI          205  GGCGCC               GGCGC^C
BciVI        1558  GTATCC               GTATCC(6/5)
BfrI         3770  CTTAAG               C^TTAAG
BglI           30  GCCGCCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2081  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2081  GCTCAGC              GC^T[ACGT]AGC
BsaAI         230  TACGTA               [CT]AC^GT[GA]
BsaWI         910  TCCGGA               [AT]^CCGG[AT]
BseAI         910  TCCGGA               T^CCGGA
BsoBI         572  CCCGAG               C^[CT]CG[GA]G
BspEI         910  TCCGGA               T^CCGGA
BspHI        4074  TCATGA               T^CATGA
BspMI         600  ACCTGC               ACCTGC(4/8)
BsrBI         809  GAGCGG               CCGCTC(-3/-3)
BsrDI         590  CATTGC               GCAATG(2/0)
BssSI         386  CTCGTG               CACGAG(-5/-1)
BstBI        3250  TTCGAA               TT^CGAA
CelII        2081  GCTCAGC              GC^T[ACGT]AGC
EagI           61  CGGCCG               C^GGCCG
EclXI          61  CGGCCG               C^GGCCG
Eco47III     2085  AGCGCT               AGC^GCT
Eco52I         61  CGGCCG               C^GGCCG
HincII       3700  GTTGAC               GT[CT]^[GA]AC
HindII       3700  GTTGAC               GT[CT]^[GA]AC
HindIII      2340  AAGCTT               A^AGCTT
KasI          205  GGCGCC               G^GCGCC
KspI           34  CCGCGG               CCGC^GG
MroI          910  TCCGGA               T^CCGGA
NarI          205  GGCGCC               GG^CGCC
NspV         3250  TTCGAA               TT^CGAA
PflFI        1532  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2466  GACTGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         4074  TCATGA               T^CATGA
SacII          34  CCGCGG               CCGC^GG
SapI         1042  GAAGAGC              GCTCTTC(1/4)
SbfI         1132  CCTGCAGG             CCTGCA^GG
SfoI          205  GGCGCC               GGC^GCC
SfuI         3250  TTCGAA               TT^CGAA
SnaBI         230  TACGTA               TAC^GTA
Sse8387I     1132  CCTGCAGG             CCTGCA^GG
SspI         4110  AATATT               AAT^ATT
Tth111I      1532  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AocI          239  CCTGAGG              CC^T[ACGT]AGG
             2415  CCTCAGG             
BsiEI          61  CGGCCG               CG[GA][CT]^CG
             3275  CGGTCG              
BsiWI         228  CGTACG               C^GTACG
              232  CGTACG              
BsmI         1295  GAATGC               GAATGC(1/-1)
             1339  GAATGC              
Bsp1407I     2816  TGTACA               T^GTACA
             4224  TGTACA              
BsrGI        2816  TGTACA               T^GTACA
             4224  TGTACA              
Bst1107I      945  GTATAC               GTA^TAC
             2656  GTATAC              
BstAPI       1543  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2176  GCAGAGCTTGC         
BstXI        2546  CCACAGTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3138  CCACACATTTGG        
BstYI        1140  AGATCC               [GA]^GATC[CT]
             3361  GGATCT              
BstZ17I       945  GTATAC               GTA^TAC
             2656  GTATAC              
Bsu36I        239  CCTGAGG              CC^T[ACGT]AGG
             2415  CCTCAGG             
DraI         4059  TTTAAA               TTT^AAA
             4126  TTTAAA              
DraIII        500  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1720  CACCGTGTG           
DrdI         1594  GACTGTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3988  GACTCTACAGTC        
Eco81I        239  CCTGAGG              CC^T[ACGT]AGG
             2415  CCTCAGG             
EcoNI        2614  CCTGTCATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3408  CCTTTGTAAGG         
EcoT22I      2588  ATGCAT               ATGCA^T
             3007  ATGCAT              
HaeII         205  GGCGCC               [GA]GCGC^[CT]
             2085  AGCGCT              
MflI         1140  AGATCC               [GA]^GATC[CT]
             3361  GGATCT              
NcoI          940  CCATGG               C^CATGG
             2142  CCATGG              
NsiI         2588  ATGCAT               ATGCA^T
             3007  ATGCAT              
PflMI        2113  CCAAATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2328  CCAGTGGCTGG         
PstI         1133  CTGCAG               CTGCA^G
             3182  CTGCAG              
SacI         2690  GAGCTC               GAGCT^C
             3109  GAGCTC              
SspBI        2816  TGTACA               T^GTACA
             4224  TGTACA              
Van91I       2113  CCAAATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2328  CCAGTGGCTGG         
XhoII        1140  AGATCC               [GA]^GATC[CT]
             3361  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        Alw44I      AlwI        AlwNI       
ApaLI       ApoI        Asp700I     AspHI       AvaII       
AvrII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BlnI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XmnI