##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I AspI AviII
BbeI BbrPI BcgI BfrI BlpI
Bpu1102I BsaAI BsaHI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrFI BsrGI BssHII BstBI BstUI
CelII Cfr10I ClaI CpoI DrdI
EagI EclXI Eco52I EcoNI EcoRV
FauI FseI FspI HgaI Hin1I
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI Tth111I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 3359 GGTACC G^GTACC
AflIII 2497 ACACGT A^C[GA][CT]GT
AhdI 3623 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1433 CCTGAGG CC^T[ACGT]AGG
Aor51HI 213 AGCGCT AGC^GCT
Asp718I 3359 GGTACC G^GTACC
AspEI 3623 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3788 CCTAGG C^CTAGG
BamHI 1198 GGATCC G^GATCC
BbsI 23 GAAGAC GAAGAC(2/6)
BclI 1144 TGATCA T^GATCA
BglI 1391 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3788 CCTAGG C^CTAGG
BpuAI 23 GAAGAC GAAGAC(2/6)
BsaBI 354 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 190 CGACCG CG[GA][CT]^CG
BsmBI 906 CGTCTC CGTCTC(1/5)
BspHI 529 TCATGA T^CATGA
Bst1107I 2867 GTATAC GTA^TAC
BstEII 2695 GGTCACC G^GT[ACGT]ACC
BstPI 2695 GGTCACC G^GT[ACGT]ACC
BstZ17I 2867 GTATAC GTA^TAC
Bsu36I 1433 CCTGAGG CC^T[ACGT]AGG
Eam1105I 3623 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 213 AGCGCT AGC^GCT
Eco81I 1433 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2695 GGTCACC G^GT[ACGT]ACC
EcoRI 751 GAATTC G^AATTC
EcoT22I 2592 ATGCAT ATGCA^T
FbaI 1144 TGATCA T^GATCA
HindIII 944 AAGCTT A^AGCTT
HpaI 3774 GTTAAC GTT^AAC
KpnI 3359 GGTACC GGTAC^C
MamI 354 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4209 CATATG CA^TATG
NheI 1796 GCTAGC G^CTAGC
NsiI 2592 ATGCAT ATGCA^T
PaeR7I 1406 CTCGAG C^TCGAG
PflMI 4069 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 731 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 529 TCATGA T^CATGA
SacI 2720 GAGCTC GAGCT^C
SmaI 363 CCCGGG CCC^GGG
SphI 1621 GCATGC GCATG^C
SwaI 4011 ATTTAAAT ATTT^AAAT
Van91I 4069 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1406 CTCGAG C^TCGAG
XmaCI 363 CCCGGG C^CCGGG
XmaI 363 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 751 GAATTC [GA]^AATT[CT]
3820 AAATTC
ApaI 1841 GGGCCC GGGCC^C
2393 GGGCCC
ApoI 751 GAATTC [GA]^AATT[CT]
3820 AAATTC
BanI 1166 GGCACC G^G[CT][GA]CC
3359 GGTACC
BbvCI 1688 CCTCAGC CCTCAGC(-5/-2)
1824 CCTCAGC
BciVI 1243 GGATAC GTATCC(6/5)
3470 GGATAC
BglII 582 AGATCT A^GATCT
4061 AGATCT
BsaI 3222 GGTCTC GGTCTC(1/5)
3712 GGTCTC
BsaWI 29 TCCGGT [AT]^CCGG[AT]
468 ACCGGA
BsmI 40 GCATTC GAATGC(1/-1)
1668 GCATTC
BsrBI 52 CCGCTC CCGCTC(-3/-3)
589 CCGCTC
BssSI 2422 CACGAG CACGAG(-5/-1)
2723 CTCGTG
BstAPI 1646 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1910 GCAGCAGCTGC
DraIII 1148 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3316 CACTCGGTG
MslI 2698 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4032 CATTTTTGTG
NspI 1621 GCATGC [GA]CATG^[CT]
3027 GCATGT
StuI 679 AGGCCT AGG^CCT
1515 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII