##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccI AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
AspEI BbsI BciVI BfrI BglII
BpuAI BsiWI BsmBI BsmI BspDI
BspHI BspLU11I Bst1107I BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI MfeI
MluI MunI NcoI NdeI NheI
NotI NruI NsiI PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SmaI
SnaBI SpeI SspI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 2999 GACGTC GACGT^C
Acc65I 876 GGTACC G^GTACC
AccIII 2468 TCCGGA T^CCGGA
AflIII 2340 ACACGT A^C[GA][CT]GT
Alw44I 905 GTGCAC G^TGCAC
Aor51HI 2770 AGCGCT AGC^GCT
ApaLI 905 GTGCAC G^TGCAC
Asp700I 3424 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 876 GGTACC G^GTACC
AviII 1619 TGCGCA TGC^GCA
AvrII 454 CCTAGG C^CTAGG
BamHI 3179 GGATCC G^GATCC
BbeI 1565 GGCGCC GGCGC^C
BcgI 1887 GCAAGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1103 TGATCA T^GATCA
BlnI 454 CCTAGG C^CTAGG
BlpI 1676 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1676 GCTCAGC GC^T[ACGT]AGC
BsaBI 2058 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2468 TCCGGA T^CCGGA
BsiEI 2217 CGACCG CG[GA][CT]^CG
Bsp1407I 3081 TGTACA T^GTACA
BspEI 2468 TCCGGA T^CCGGA
BsrDI 2222 GCAATG GCAATG(2/0)
BsrGI 3081 TGTACA T^GTACA
BssHII 100 GCGCGC G^CGCGC
BssSI 1864 CTCGTG CACGAG(-5/-1)
BstBI 3161 TTCGAA TT^CGAA
CelII 1676 GCTCAGC GC^T[ACGT]AGC
DraIII 1861 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2017 GACGACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2770 AGCGCT AGC^GCT
EcoNI 386 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1103 TGATCA T^GATCA
FspI 1619 TGCGCA TGC^GCA
HgaI 1981 GACGC GACGC(5/10)
KasI 1565 GGCGCC G^GCGCC
KpnI 876 GGTACC GGTAC^C
KspI 7 CCGCGG CCGC^GG
MamI 2058 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2468 TCCGGA T^CCGGA
MseI 998 TTAA T^TAA
NaeI 13 GCCGGC GCC^GGC
NarI 1565 GGCGCC GG^CGCC
NgoMIV 13 GCCGGC G^CCGGC
NspV 3161 TTCGAA TT^CGAA
PaeR7I 2967 CTCGAG C^TCGAG
PshAI 3383 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2209 GAGCTC GAGCT^C
SacII 7 CCGCGG CCGC^GG
SfoI 1565 GGCGCC GGC^GCC
SfuI 3161 TTCGAA TT^CGAA
SgrAI 472 CACCGGCG C[GA]^CCGG[CT]G
SphI 3405 GCATGC GCATG^C
SspBI 3081 TGTACA T^GTACA
TfiI 1138 GATTC G^A[AT]TC
Tru9I 998 TTAA T^TAA
Tsp509I 695 AATT ^AATT
XhoI 2967 CTCGAG C^TCGAG
XmnI 3424 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 850 CCTGAGG CC^T[ACGT]AGG
3055 CCTGAGG
AspI 2020 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3342 GACCCCGTC
BbrPI 1230 CACGTG CAC^GTG
2341 CACGTG
BsaAI 1230 CACGTG [CT]AC^GT[GA]
2341 CACGTG
BsaI 2025 GGTCTC GGTCTC(1/5)
2241 GGTCTC
BsaWI 1392 TCCGGT [AT]^CCGG[AT]
2468 TCCGGA
BstAPI 631 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1870 GCAGCCCATGC
BstEII 107 GGTTACC G^GT[ACGT]ACC
459 GGTCACC
BstPI 107 GGTTACC G^GT[ACGT]ACC
459 GGTCACC
Bsu36I 850 CCTGAGG CC^T[ACGT]AGG
3055 CCTGAGG
Eco81I 850 CCTGAGG CC^T[ACGT]AGG
3055 CCTGAGG
EcoO65I 107 GGTTACC G^GT[ACGT]ACC
459 GGTCACC
PflFI 2020 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3342 GACCCCGTC
PmaCI 1230 CACGTG CAC^GTG
2341 CACGTG
PmlI 1230 CACGTG CAC^GTG
2341 CACGTG
SfiI 735 GGCCGAACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1124 GGCCTGCAGGGCC
SmlI 2076 CTTGAG C^T[CT][GA]AG
2967 CTCGAG
StuI 925 AGGCCT AGG^CCT
1493 AGGCCT
Tth111I 2020 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3342 GACCCCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI Sse8387I StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII