################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AccI AclI AgeI Aor51HI AscI AspI AviII AvrII BamHI BbeI BciVI BlnI BsiWI BsmBI BspDI BsrBI BsrDI BssHII Bst1107I BstZ17I ClaI DrdI Eco47III EcoRV FseI FspI KasI MfeI MluI MunI NaeI NarI NcoI NgoMIV NotI NruI PacI PflFI PinAI PmeI PshAI Psp1406I PvuI SalI SbfI SfiI SfoI SgrAI SnaBI SphI Sse8387I Tth111I XcmI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 724 GACGTC GACGT^C Acc65I 635 GGTACC G^GTACC AccIII 1591 TCCGGA T^CCGGA AflII 3932 CTTAAG C^TTAAG Alw44I 2965 GTGCAC G^TGCAC ApaLI 2965 GTGCAC G^TGCAC AseI 2872 ATTAAT AT^TAAT AsnI 2872 ATTAAT AT^TAAT Asp718I 635 GGTACC G^GTACC BanI 635 GGTACC G^G[CT][GA]CC BbvCI 290 GCTGAGG CCTCAGC(-5/-2) BcgI 823 CGAATGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1169 TGATCA T^GATCA BfrI 3932 CTTAAG C^TTAAG BglI 189 GCCCCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 2523 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1404 GAGACC GGTCTC(1/5) BsaWI 1591 TCCGGA [AT]^CCGG[AT] BseAI 1591 TCCGGA T^CCGGA BsiEI 643 CGGCCG CG[GA][CT]^CG BspEI 1591 TCCGGA T^CCGGA BspHI 4613 TCATGA T^CATGA BspLU11I 3775 ACATGT A^CATGT BspMI 3811 ACCTGC ACCTGC(4/8) BssSI 1399 CTCGTG CACGAG(-5/-1) BstBI 941 TTCGAA TT^CGAA BstEII 2118 GGTAACC G^GT[ACGT]ACC BstPI 2118 GGTAACC G^GT[ACGT]ACC CpoI 199 CGGACCG CG^G[AT]CCG EagI 643 CGGCCG C^GGCCG EclXI 643 CGGCCG C^GGCCG Eco52I 643 CGGCCG C^GGCCG EcoO65I 2118 GGTAACC G^GT[ACGT]ACC EcoRI 4131 GAATTC G^AATTC EcoT22I 1109 ATGCAT ATGCA^T FbaI 1169 TGATCA T^GATCA KpnI 635 GGTACC GGTAC^C MamI 2523 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1591 TCCGGA T^CCGGA NdeI 1432 CATATG CA^TATG NheI 2277 GCTAGC G^CTAGC NsiI 1109 ATGCAT ATGCA^T NspV 941 TTCGAA TT^CGAA PaeR7I 3410 CTCGAG C^TCGAG PshBI 2872 ATTAAT AT^TAAT RcaI 4613 TCATGA T^CATGA RsrII 199 CGGACCG CG^G[AT]CCG SacI 453 GAGCTC GAGCT^C SfuI 941 TTCGAA TT^CGAA SpeI 4424 ACTAGT A^CTAGT SwaI 3851 ATTTAAAT ATTT^AAAT XbaI 1726 TCTAGA T^CTAGA XhoI 3410 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AcyI 225 GGCGTC G[GA]^CG[CT]C 724 GACGTC AhdI 1252 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2770 GACAATGTGTC AlwI 3331 GATCC GGATC(4/5) 789 GGATC AocI 192 CCTGAGG CC^T[ACGT]AGG 2010 CCTCAGG ApaI 156 GGGCCC GGGCC^C 3419 GGGCCC Asp700I 2813 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4319 GAAAGTTTTC AspEI 1252 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2770 GACAATGTGTC BbrPI 1782 CACGTG CAC^GTG 4119 CACGTG BsaHI 225 GGCGTC G[GA]^CG[CT]C 724 GACGTC BsgI 889 GTGCAG GTGCAG(16/14) 1147 GTGCAG Bsp1407I 3260 TGTACA T^GTACA 3906 TGTACA BsrFI 311 GCCGGT [GA]^CCGG[CT] 641 ACCGGC BsrGI 3260 TGTACA T^GTACA 3906 TGTACA BstAPI 462 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2413 GCAACGGCTGC BstXI 1703 CCAATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3594 CCAATACTGTGG Bsu36I 192 CCTGAGG CC^T[ACGT]AGG 2010 CCTCAGG Cfr10I 311 GCCGGT [GA]^CCGG[CT] 641 ACCGGC Eam1105I 1252 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2770 GACAATGTGTC Eco81I 192 CCTGAGG CC^T[ACGT]AGG 2010 CCTCAGG EcoT14I 1133 CCTTGG C^C[AT][AT]GG 4200 CCAAGG HgaI 226 GCGTC GACGC(5/10) 896 GCGTC Hin1I 225 GGCGTC G[GA]^CG[CT]C 724 GACGTC HindIII 1928 AAGCTT A^AGCTT 2918 AAGCTT HpaI 1492 GTTAAC GTT^AAC 1554 GTTAAC KspI 54 CCGCGG CCGC^GG 203 CCGCGG MslI 2968 CACTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3155 CACAAGAGTG PmaCI 1782 CACGTG CAC^GTG 4119 CACGTG PmlI 1782 CACGTG CAC^GTG 4119 CACGTG PstI 1162 CTGCAG CTGCA^G 1239 CTGCAG SacII 54 CCGCGG CCGC^GG 203 CCGCGG SapI 2389 GAAGAGC GCTCTTC(1/4) 2495 GCTCTTC ScaI 2633 AGTACT AGT^ACT 4279 AGTACT SexAI 611 ACCAGGT A^CC[AT]GGT 1407 ACCTGGT SspBI 3260 TGTACA T^GTACA 3906 TGTACA StuI 444 AGGCCT AGG^CCT 623 AGGCCT StyI 1133 CCTTGG C^C[AT][AT]GG 4200 CCAAGG XmnI 2813 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4319 GAAAGTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwNI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI