##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccI AclI AgeI Aor51HI AscI
AspI AviII AvrII BamHI BbeI
BciVI BlnI BsiWI BsmBI BspDI
BsrBI BsrDI BssHII Bst1107I BstZ17I
ClaI DrdI Eco47III EcoRV FseI
FspI KasI MfeI MluI MunI
NaeI NarI NcoI NgoMIV NotI
NruI PacI PflFI PinAI PmeI
PshAI Psp1406I PvuI SalI SbfI
SfiI SfoI SgrAI SnaBI SphI
Sse8387I Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 724 GACGTC GACGT^C
Acc65I 635 GGTACC G^GTACC
AccIII 1591 TCCGGA T^CCGGA
AflII 3932 CTTAAG C^TTAAG
Alw44I 2965 GTGCAC G^TGCAC
ApaLI 2965 GTGCAC G^TGCAC
AseI 2872 ATTAAT AT^TAAT
AsnI 2872 ATTAAT AT^TAAT
Asp718I 635 GGTACC G^GTACC
BanI 635 GGTACC G^G[CT][GA]CC
BbvCI 290 GCTGAGG CCTCAGC(-5/-2)
BcgI 823 CGAATGGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1169 TGATCA T^GATCA
BfrI 3932 CTTAAG C^TTAAG
BglI 189 GCCCCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2523 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1404 GAGACC GGTCTC(1/5)
BsaWI 1591 TCCGGA [AT]^CCGG[AT]
BseAI 1591 TCCGGA T^CCGGA
BsiEI 643 CGGCCG CG[GA][CT]^CG
BspEI 1591 TCCGGA T^CCGGA
BspHI 4613 TCATGA T^CATGA
BspLU11I 3775 ACATGT A^CATGT
BspMI 3811 ACCTGC ACCTGC(4/8)
BssSI 1399 CTCGTG CACGAG(-5/-1)
BstBI 941 TTCGAA TT^CGAA
BstEII 2118 GGTAACC G^GT[ACGT]ACC
BstPI 2118 GGTAACC G^GT[ACGT]ACC
CpoI 199 CGGACCG CG^G[AT]CCG
EagI 643 CGGCCG C^GGCCG
EclXI 643 CGGCCG C^GGCCG
Eco52I 643 CGGCCG C^GGCCG
EcoO65I 2118 GGTAACC G^GT[ACGT]ACC
EcoRI 4131 GAATTC G^AATTC
EcoT22I 1109 ATGCAT ATGCA^T
FbaI 1169 TGATCA T^GATCA
KpnI 635 GGTACC GGTAC^C
MamI 2523 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1591 TCCGGA T^CCGGA
NdeI 1432 CATATG CA^TATG
NheI 2277 GCTAGC G^CTAGC
NsiI 1109 ATGCAT ATGCA^T
NspV 941 TTCGAA TT^CGAA
PaeR7I 3410 CTCGAG C^TCGAG
PshBI 2872 ATTAAT AT^TAAT
RcaI 4613 TCATGA T^CATGA
RsrII 199 CGGACCG CG^G[AT]CCG
SacI 453 GAGCTC GAGCT^C
SfuI 941 TTCGAA TT^CGAA
SpeI 4424 ACTAGT A^CTAGT
SwaI 3851 ATTTAAAT ATTT^AAAT
XbaI 1726 TCTAGA T^CTAGA
XhoI 3410 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 225 GGCGTC G[GA]^CG[CT]C
724 GACGTC
AhdI 1252 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2770 GACAATGTGTC
AlwI 3331 GATCC GGATC(4/5)
789 GGATC
AocI 192 CCTGAGG CC^T[ACGT]AGG
2010 CCTCAGG
ApaI 156 GGGCCC GGGCC^C
3419 GGGCCC
Asp700I 2813 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4319 GAAAGTTTTC
AspEI 1252 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2770 GACAATGTGTC
BbrPI 1782 CACGTG CAC^GTG
4119 CACGTG
BsaHI 225 GGCGTC G[GA]^CG[CT]C
724 GACGTC
BsgI 889 GTGCAG GTGCAG(16/14)
1147 GTGCAG
Bsp1407I 3260 TGTACA T^GTACA
3906 TGTACA
BsrFI 311 GCCGGT [GA]^CCGG[CT]
641 ACCGGC
BsrGI 3260 TGTACA T^GTACA
3906 TGTACA
BstAPI 462 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2413 GCAACGGCTGC
BstXI 1703 CCAATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3594 CCAATACTGTGG
Bsu36I 192 CCTGAGG CC^T[ACGT]AGG
2010 CCTCAGG
Cfr10I 311 GCCGGT [GA]^CCGG[CT]
641 ACCGGC
Eam1105I 1252 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2770 GACAATGTGTC
Eco81I 192 CCTGAGG CC^T[ACGT]AGG
2010 CCTCAGG
EcoT14I 1133 CCTTGG C^C[AT][AT]GG
4200 CCAAGG
HgaI 226 GCGTC GACGC(5/10)
896 GCGTC
Hin1I 225 GGCGTC G[GA]^CG[CT]C
724 GACGTC
HindIII 1928 AAGCTT A^AGCTT
2918 AAGCTT
HpaI 1492 GTTAAC GTT^AAC
1554 GTTAAC
KspI 54 CCGCGG CCGC^GG
203 CCGCGG
MslI 2968 CACTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3155 CACAAGAGTG
PmaCI 1782 CACGTG CAC^GTG
4119 CACGTG
PmlI 1782 CACGTG CAC^GTG
4119 CACGTG
PstI 1162 CTGCAG CTGCA^G
1239 CTGCAG
SacII 54 CCGCGG CCGC^GG
203 CCGCGG
SapI 2389 GAAGAGC GCTCTTC(1/4)
2495 GCTCTTC
ScaI 2633 AGTACT AGT^ACT
4279 AGTACT
SexAI 611 ACCAGGT A^CC[AT]GGT
1407 ACCTGGT
SspBI 3260 TGTACA T^GTACA
3906 TGTACA
StuI 444 AGGCCT AGG^CCT
623 AGGCCT
StyI 1133 CCTTGG C^C[AT][AT]GG
4200 CCAAGG
XmnI 2813 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4319 GAAAGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwNI ApoI AspHI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI