##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AvaI AvrII BamHI BbeI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BseAI BsiWI BsoBI BspDI BspEI
BspMI BssHII Bsu36I CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT22I FseI
HpaI KasI KspI MluI MroI
NarI NotI NruI NsiI PacI
PaeR7I PinAI PmeI PpuMI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I StuI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 1569 CGCG CG^CG
AcyI 850 GACGCC G[GA]^CG[CT]C
AflIII 426 ACATGT A^C[GA][CT]GT
AhdI 3401 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1447 GGGCCC GGGCC^C
AseI 2413 ATTAAT AT^TAAT
AsnI 2413 ATTAAT AT^TAAT
AspEI 3401 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 917 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 321 TGCGCA TGC^GCA
BbrPI 1354 CACGTG CAC^GTG
BbvCI 1395 GCTGAGG CCTCAGC(-5/-2)
BcgI 1736 GCACGGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 1580 CTGGAG CTGGAG(16/14)
BsaBI 1503 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 850 GACGCC G[GA]^CG[CT]C
BseRI 1861 GAGGAG GAGGAG(10/8)
BsiEI 483 CGACCG CG[GA][CT]^CG
BsmBI 848 GAGACG CGTCTC(1/5)
BsmFI 3324 GTCCC GGGAC(10/14)
BspLU11I 426 ACATGT A^CATGT
BsrBI 3047 GAGCGG CCGCTC(-3/-3)
Bst1107I 4096 GTATAC GTA^TAC
BstBI 1034 TTCGAA TT^CGAA
BstUI 1569 CGCG CG^CG
BstXI 1928 CCATCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4096 GTATAC GTA^TAC
DraII 1447 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 4067 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1195 GACTCGGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 357 CCATGG C^C[GA][CT]GG
Eam1105I 3401 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1447 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 3257 GATATC GAT^ATC
FspI 321 TGCGCA TGC^GCA
HaeII 990 GGCGCT [GA]GCGC^[CT]
HgaI 850 GACGC GACGC(5/10)
Hin1I 850 GACGCC G[GA]^CG[CT]C
HindIII 1120 AAGCTT A^AGCTT
MamI 1503 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1682 CAATTG C^AATTG
MunI 1682 CAATTG C^AATTG
MvnI 1569 CGCG CG^CG
NaeI 1792 GCCGGC GCC^GGC
NcoI 357 CCATGG C^CATGG
NdeI 2405 CATATG CA^TATG
NgoMIV 1792 GCCGGC G^CCGGC
NheI 3226 GCTAGC G^CTAGC
NspV 1034 TTCGAA TT^CGAA
PflFI 917 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3286 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1354 CACGTG CAC^GTG
PmlI 1354 CACGTG CAC^GTG
PshAI 892 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2413 ATTAAT AT^TAAT
SfuI 1034 TTCGAA TT^CGAA
SmlI 1171 CTCAAG C^T[CT][GA]AG
SphI 3535 GCATGC GCATG^C
SwaI 650 ATTTAAAT ATTT^AAAT
Tth111I 917 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3286 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2956 GGTACC G^GTACC
2973 GGTACC
AccI 3142 GTAGAC GT^[AC][GT]AC
4096 GTATAC
AlwNI 2092 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
3874 CAGTTGCTG
Asp700I 1118 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4163 GAACCTTTTC
Asp718I 2956 GGTACC G^GTACC
2973 GGTACC
BalI 309 TGGCCA TGG^CCA
354 TGGCCA
BanII 106 GAGCCC G[GA]GC[CT]^C
1447 GGGCCC
BclI 249 TGATCA T^GATCA
1508 TGATCA
BcnI 109 CCCGG CC^[GC]GG
276 CCGGG
BsaAI 570 CACGTA [CT]AC^GT[GA]
1354 CACGTG
BsaI 130 GAGACC GGTCTC(1/5)
3945 GGTCTC
BsaWI 558 ACCGGA [AT]^CCGG[AT]
1149 ACCGGA
Bsp1407I 297 TGTACA T^GTACA
1688 TGTACA
BsrFI 1214 ACCGGC [GA]^CCGG[CT]
1792 GCCGGC
BsrGI 297 TGTACA T^GTACA
1688 TGTACA
BssSI 1345 CACGAG CACGAG(-5/-1)
2020 CTCGTG
BstEII 1852 GGTGACC G^GT[ACGT]ACC
4038 GGTCACC
BstPI 1852 GGTGACC G^GT[ACGT]ACC
4038 GGTCACC
Cfr10I 1214 ACCGGC [GA]^CCGG[CT]
1792 GCCGGC
EaeI 309 TGGCCA [CT]^GGCC[GA]
354 TGGCCA
EcoO65I 1852 GGTGACC G^GT[ACGT]ACC
4038 GGTCACC
FauI 1570 GCGGG CCCGC(4/6)
3743 GCGGG
FbaI 249 TGATCA T^GATCA
1508 TGATCA
HincII 3618 GTCAAC GT[CT]^[GA]AC
4319 GTCAAC
HindII 3618 GTCAAC GT[CT]^[GA]AC
4319 GTCAAC
KpnI 2956 GGTACC GGTAC^C
2973 GGTACC
MluNI 309 TGGCCA TGG^CCA
354 TGGCCA
MscI 309 TGGCCA TGG^CCA
354 TGGCCA
MspA1I 3162 CAGCTG C[AC]G^C[GT]G
4117 CAGCTG
NciI 109 CCCGG CC^[GC]GG
276 CCGGG
PvuII 3162 CAGCTG CAG^CTG
4117 CAGCTG
ScaI 2135 AGTACT AGT^ACT
2794 AGTACT
SexAI 228 ACCAGGT A^CC[AT]GGT
3402 ACCAGGT
SpeI 2243 ACTAGT A^CTAGT
3146 ACTAGT
SspBI 297 TGTACA T^GTACA
1688 TGTACA
XbaI 2534 TCTAGA T^CTAGA
2756 TCTAGA
XmnI 1118 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4163 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BbsI
BbvI BfaI BglI BmyI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BspHI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII