################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AvaI AvrII BamHI BbeI BciVI BfrI BglII BlnI BlpI Bpu1102I BseAI BsiWI BsoBI BspDI BspEI BspMI BssHII Bsu36I CelII ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FseI HpaI KasI KspI MluI MroI NarI NotI NruI NsiI PacI PaeR7I PinAI PmeI PpuMI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I StuI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccII 1569 CGCG CG^CG AcyI 850 GACGCC G[GA]^CG[CT]C AflIII 426 ACATGT A^C[GA][CT]GT AhdI 3401 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1447 GGGCCC GGGCC^C AseI 2413 ATTAAT AT^TAAT AsnI 2413 ATTAAT AT^TAAT AspEI 3401 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 917 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 321 TGCGCA TGC^GCA BbrPI 1354 CACGTG CAC^GTG BbvCI 1395 GCTGAGG CCTCAGC(-5/-2) BcgI 1736 GCACGGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 1580 CTGGAG CTGGAG(16/14) BsaBI 1503 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 850 GACGCC G[GA]^CG[CT]C BseRI 1861 GAGGAG GAGGAG(10/8) BsiEI 483 CGACCG CG[GA][CT]^CG BsmBI 848 GAGACG CGTCTC(1/5) BsmFI 3324 GTCCC GGGAC(10/14) BspLU11I 426 ACATGT A^CATGT BsrBI 3047 GAGCGG CCGCTC(-3/-3) Bst1107I 4096 GTATAC GTA^TAC BstBI 1034 TTCGAA TT^CGAA BstUI 1569 CGCG CG^CG BstXI 1928 CCATCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 4096 GTATAC GTA^TAC DraII 1447 GGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 4067 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1195 GACTCGGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 357 CCATGG C^C[GA][CT]GG Eam1105I 3401 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 1447 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 3257 GATATC GAT^ATC FspI 321 TGCGCA TGC^GCA HaeII 990 GGCGCT [GA]GCGC^[CT] HgaI 850 GACGC GACGC(5/10) Hin1I 850 GACGCC G[GA]^CG[CT]C HindIII 1120 AAGCTT A^AGCTT MamI 1503 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1682 CAATTG C^AATTG MunI 1682 CAATTG C^AATTG MvnI 1569 CGCG CG^CG NaeI 1792 GCCGGC GCC^GGC NcoI 357 CCATGG C^CATGG NdeI 2405 CATATG CA^TATG NgoMIV 1792 GCCGGC G^CCGGC NheI 3226 GCTAGC G^CTAGC NspV 1034 TTCGAA TT^CGAA PflFI 917 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3286 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1354 CACGTG CAC^GTG PmlI 1354 CACGTG CAC^GTG PshAI 892 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2413 ATTAAT AT^TAAT SfuI 1034 TTCGAA TT^CGAA SmlI 1171 CTCAAG C^T[CT][GA]AG SphI 3535 GCATGC GCATG^C SwaI 650 ATTTAAAT ATTT^AAAT Tth111I 917 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3286 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 2956 GGTACC G^GTACC 2973 GGTACC AccI 3142 GTAGAC GT^[AC][GT]AC 4096 GTATAC AlwNI 2092 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG 3874 CAGTTGCTG Asp700I 1118 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4163 GAACCTTTTC Asp718I 2956 GGTACC G^GTACC 2973 GGTACC BalI 309 TGGCCA TGG^CCA 354 TGGCCA BanII 106 GAGCCC G[GA]GC[CT]^C 1447 GGGCCC BclI 249 TGATCA T^GATCA 1508 TGATCA BcnI 109 CCCGG CC^[GC]GG 276 CCGGG BsaAI 570 CACGTA [CT]AC^GT[GA] 1354 CACGTG BsaI 130 GAGACC GGTCTC(1/5) 3945 GGTCTC BsaWI 558 ACCGGA [AT]^CCGG[AT] 1149 ACCGGA Bsp1407I 297 TGTACA T^GTACA 1688 TGTACA BsrFI 1214 ACCGGC [GA]^CCGG[CT] 1792 GCCGGC BsrGI 297 TGTACA T^GTACA 1688 TGTACA BssSI 1345 CACGAG CACGAG(-5/-1) 2020 CTCGTG BstEII 1852 GGTGACC G^GT[ACGT]ACC 4038 GGTCACC BstPI 1852 GGTGACC G^GT[ACGT]ACC 4038 GGTCACC Cfr10I 1214 ACCGGC [GA]^CCGG[CT] 1792 GCCGGC EaeI 309 TGGCCA [CT]^GGCC[GA] 354 TGGCCA EcoO65I 1852 GGTGACC G^GT[ACGT]ACC 4038 GGTCACC FauI 1570 GCGGG CCCGC(4/6) 3743 GCGGG FbaI 249 TGATCA T^GATCA 1508 TGATCA HincII 3618 GTCAAC GT[CT]^[GA]AC 4319 GTCAAC HindII 3618 GTCAAC GT[CT]^[GA]AC 4319 GTCAAC KpnI 2956 GGTACC GGTAC^C 2973 GGTACC MluNI 309 TGGCCA TGG^CCA 354 TGGCCA MscI 309 TGGCCA TGG^CCA 354 TGGCCA MspA1I 3162 CAGCTG C[AC]G^C[GT]G 4117 CAGCTG NciI 109 CCCGG CC^[GC]GG 276 CCGGG PvuII 3162 CAGCTG CAG^CTG 4117 CAGCTG ScaI 2135 AGTACT AGT^ACT 2794 AGTACT SexAI 228 ACCAGGT A^CC[AT]GGT 3402 ACCAGGT SpeI 2243 ACTAGT A^CTAGT 3146 ACTAGT SspBI 297 TGTACA T^GTACA 1688 TGTACA XbaI 2534 TCTAGA T^CTAGA 2756 TCTAGA XmnI 1118 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4163 GAACCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BbsI BbvI BfaI BglI BmyI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmI Bsp1286I BspHI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII