##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
ApaI AscI AseI AsnI AviII
AvrII BbeI BbrPI BcgI BclI
BglII BlnI BsaBI BseAI BsiWI
BsmI BspDI BspEI BspLU11I BssHII
BssSI BstBI ClaI DraIII Eco47III
EcoNI EcoRI EcoRV FbaI FseI
FspI HaeII HpaI KasI KspI
MamI MluI MroI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SacII
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SphI Sse8387I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 1884 GGTACC G^GTACC
AcsI 3773 AAATTT [GA]^AATT[CT]
AcyI 1243 GGCGTC G[GA]^CG[CT]C
AflII 51 CTTAAG C^TTAAG
AflIII 2358 ACGTGT A^C[GA][CT]GT
AhdI 666 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1571 CCTGAGG CC^T[ACGT]AGG
ApoI 3773 AAATTT [GA]^AATT[CT]
Asp718I 1884 GGTACC G^GTACC
AspEI 666 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 991 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 66 GGATAC GTATCC(6/5)
BfrI 51 CTTAAG C^TTAAG
BlpI 3015 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3015 GCTGAGC GC^T[ACGT]AGC
BsaAI 1691 TACGTA [CT]AC^GT[GA]
BsaHI 1243 GGCGTC G[GA]^CG[CT]C
BsaI 1112 GGTCTC GGTCTC(1/5)
BsiEI 2421 CGGCCG CG[GA][CT]^CG
Bsp1407I 1555 TGTACA T^GTACA
BsrFI 3091 ACCGGC [GA]^CCGG[CT]
BsrGI 1555 TGTACA T^GTACA
Bst1107I 37 GTATAC GTA^TAC
BstEII 84 GGTCACC G^GT[ACGT]ACC
BstPI 84 GGTCACC G^GT[ACGT]ACC
BstZ17I 37 GTATAC GTA^TAC
Bsu36I 1571 CCTGAGG CC^T[ACGT]AGG
CelII 3015 GCTGAGC GC^T[ACGT]AGC
CfoI 1282 GCGC GCG^C
Cfr10I 3091 ACCGGC [GA]^CCGG[CT]
CpoI 995 CGGTCCG CG^G[AT]CCG
EagI 2421 CGGCCG C^GGCCG
Eam1105I 666 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2421 CGGCCG C^GGCCG
Eco52I 2421 CGGCCG C^GGCCG
Eco81I 1571 CCTGAGG CC^T[ACGT]AGG
EcoO65I 84 GGTCACC G^GT[ACGT]ACC
EcoT22I 526 ATGCAT ATGCA^T
HhaI 1282 GCGC GCG^C
Hin1I 1243 GGCGTC G[GA]^CG[CT]C
HinP1I 1282 GCGC G^CGC
HindIII 2115 AAGCTT A^AGCTT
KpnI 1884 GGTACC GGTAC^C
MfeI 3434 CAATTG C^AATTG
MunI 3434 CAATTG C^AATTG
NcoI 2205 CCATGG C^CATGG
NdeI 3406 CATATG CA^TATG
NheI 2521 GCTAGC G^CTAGC
NsiI 526 ATGCAT ATGCA^T
PflFI 991 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 412 GGGACCT [GA]G^G[AT]CC[CT]
RsrII 995 CGGTCCG CG^G[AT]CCG
SapI 1845 GCTCTTC GCTCTTC(1/4)
SfiI 306 GGCCACAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 1691 TACGTA TAC^GTA
SpeI 427 ACTAGT A^CTAGT
SspBI 1555 TGTACA T^GTACA
SwaI 3726 ATTTAAAT ATTT^AAAT
Tth111I 991 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 37 GTATAC GT^[AC][GT]AC
3123 GTAGAC
Alw44I 728 GTGCAC G^TGCAC
842 GTGCAC
ApaLI 728 GTGCAC G^TGCAC
842 GTGCAC
BbvCI 893 CCTCAGC CCTCAGC(-5/-2)
1229 CCTCAGC
BpmI 2156 CTCCAG CTGGAG(16/14)
825 CTGGAG
BsaWI 566 ACCGGA [AT]^CCGG[AT]
2256 TCCGGT
BsmBI 91 GAGACG CGTCTC(1/5)
2475 GAGACG
BspHI 1425 TCATGA T^CATGA
1728 TCATGA
DraI 3302 TTTAAA TTT^AAA
3727 TTTAAA
HgaI 963 GCGTC GACGC(5/10)
1244 GCGTC
HincII 298 GTTGAC GT[CT]^[GA]AC
1150 GTTGAC
HindII 298 GTTGAC GT[CT]^[GA]AC
1150 GTTGAC
MspA1I 775 CAGCTG C[AC]G^C[GT]G
2467 CAGCTG
PvuII 775 CAGCTG CAG^CTG
2467 CAGCTG
RcaI 1425 TCATGA T^CATGA
1728 TCATGA
SacI 1211 GAGCTC GAGCT^C
1448 GAGCTC
SmlI 51 CTTAAG C^T[CT][GA]AG
259 CTCAAG
StuI 185 AGGCCT AGG^CCT
1528 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Asp700I AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI