##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI Aor51HI AscI Asp718I AviII
BbeI BglII BsaHI BsiEI BsiWI
BspDI BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CfoI
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MluI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SapI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 3882 GTCTAC GT^[AC][GT]AC
AflII 2789 CTTAAG C^TTAAG
AhdI 3927 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 48 GTGCAC G^TGCAC
ApaLI 48 GTGCAC G^TGCAC
AspEI 3927 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3051 GGATCC G^GATCC
BanI 2751 GGCACC G^G[CT][GA]CC
BbrPI 211 CACGTG CAC^GTG
BcgI 1257 GCATTTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 28 TGATCA T^GATCA
BfrI 2789 CTTAAG C^TTAAG
BsaAI 211 CACGTG [CT]AC^GT[GA]
BsaBI 1345 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1453 CGTCTC CGTCTC(1/5)
Bsp1407I 1246 TGTACA T^GTACA
BspHI 1406 TCATGA T^CATGA
BsrFI 1641 ACCGGC [GA]^CCGG[CT]
BsrGI 1246 TGTACA T^GTACA
BssSI 1265 CTCGTG CACGAG(-5/-1)
Cfr10I 1641 ACCGGC [GA]^CCGG[CT]
DrdI 719 GACTAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3927 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 3308 ATGCAT ATGCA^T
FbaI 28 TGATCA T^GATCA
HgaI 1410 GACGC GACGC(5/10)
HincII 1551 GTTGAC GT[CT]^[GA]AC
HindII 1551 GTTGAC GT[CT]^[GA]AC
MaeII 212 ACGT A^CGT
MamI 1345 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3219 CAATTG C^AATTG
MunI 3219 CAATTG C^AATTG
NdeI 978 CATATG CA^TATG
NsiI 3308 ATGCAT ATGCA^T
PaeR7I 3317 CTCGAG C^TCGAG
PflMI 1182 CCAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 211 CACGTG CAC^GTG
PmlI 211 CACGTG CAC^GTG
RcaI 1406 TCATGA T^CATGA
SacI 2996 GAGCTC GAGCT^C
SbfI 1858 CCTGCAGG CCTGCA^GG
ScaI 1776 AGTACT AGT^ACT
SfiI 2960 GGCCTCTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1858 CCTGCAGG CCTGCA^GG
SspBI 1246 TGTACA T^GTACA
Van91I 1182 CCAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2608 TCTAGA T^CTAGA
XhoI 3317 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 94 TCCGGA T^CCGGA
1719 TCCGGA
ApaI 3241 GGGCCC GGGCC^C
3356 GGGCCC
AseI 2229 ATTAAT AT^TAAT
3624 ATTAAT
AsnI 2229 ATTAAT AT^TAAT
3624 ATTAAT
AspI 159 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
789 GACCCTGTC
BbvCI 1392 CCTCAGC CCTCAGC(-5/-2)
1086 GCTGAGG
BciVI 216 GTATCC GTATCC(6/5)
3867 GTATCC
BglI 2961 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4192 GCCCAGGAGGC
BsaWI 94 TCCGGA [AT]^CCGG[AT]
1719 TCCGGA
BseAI 94 TCCGGA T^CCGGA
1719 TCCGGA
BsmI 1535 GAATGC GAATGC(1/-1)
360 GCATTC
BspEI 94 TCCGGA T^CCGGA
1719 TCCGGA
DsaI 2364 CCATGG C^C[GA][CT]GG
3065 CCATGG
EarI 2452 CTCTTC CTCTTC(1/4)
83 GAAGAG
Ksp632I 2452 CTCTTC CTCTTC(1/4)
83 GAAGAG
MroI 94 TCCGGA T^CCGGA
1719 TCCGGA
NcoI 2364 CCATGG C^CATGG
3065 CCATGG
PflFI 159 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
789 GACCCTGTC
PshAI 2394 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2536 GACCCCAGTC
PshBI 2229 ATTAAT AT^TAAT
3624 ATTAAT
SmaI 580 CCCGGG CCC^GGG
1460 CCCGGG
SphI 523 GCATGC GCATG^C
2411 GCATGC
TaqI 1709 TCGA T^CGA
3318 TCGA
Tth111I 159 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
789 GACCCTGTC
TthHB8I 1709 TCGA T^CGA
3318 TCGA
XmaCI 580 CCCGGG C^CCGGG
1460 CCCGGG
XmaI 580 CCCGGG C^CCGGG
1460 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII AvrII BalI
BanII BbsI BbvI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
Cfr13I DdeI DpnII DraI DraII
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmnI