##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I Asp718I AviII BbeI BglII
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BstAPI
BstBI CelII ClaI CpoI DraI
EcoRV EcoT22I FseI FspI HpaI
KasI KpnI KspI MamI MluI
MroI NarI NdeI NheI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AcyI 1679 GACGCC G[GA]^CG[CT]C
AflII 3426 CTTAAG C^TTAAG
AhdI 605 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2406 AGCGCT AGC^GCT
AspEI 605 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2270 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1039 CCTAGG C^CTAGG
BamHI 3453 GGATCC G^GATCC
BbrPI 2240 CACGTG CAC^GTG
BbvCI 596 GCTGAGG CCTCAGC(-5/-2)
BcgI 3013 GCAGGCTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1950 GTATCC GTATCC(6/5)
BclI 258 TGATCA T^GATCA
BfrI 3426 CTTAAG C^TTAAG
BlnI 1039 CCTAGG C^CTAGG
BsaHI 1679 GACGCC G[GA]^CG[CT]C
BsiEI 845 CGGCCG CG[GA][CT]^CG
BspHI 643 TCATGA T^CATGA
BsrBI 848 CCGCTC CCGCTC(-3/-3)
BsrDI 2746 CATTGC GCAATG(2/0)
BssHII 592 GCGCGC G^CGCGC
BssSI 3167 CTCGTG CACGAG(-5/-1)
Bst1107I 752 GTATAC GTA^TAC
BstEII 3727 GGTGACC G^GT[ACGT]ACC
BstPI 3727 GGTGACC G^GT[ACGT]ACC
BstZ17I 752 GTATAC GTA^TAC
DrdI 2733 GACCTGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 845 CGGCCG C^GGCCG
Eam1105I 605 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 845 CGGCCG C^GGCCG
Eco47III 2406 AGCGCT AGC^GCT
Eco52I 845 CGGCCG C^GGCCG
EcoO65I 3727 GGTGACC G^GT[ACGT]ACC
FbaI 258 TGATCA T^GATCA
Hin1I 1679 GACGCC G[GA]^CG[CT]C
HincII 2856 GTCAAC GT[CT]^[GA]AC
HindII 2856 GTCAAC GT[CT]^[GA]AC
MfeI 728 CAATTG C^AATTG
MunI 728 CAATTG C^AATTG
NaeI 327 GCCGGC GCC^GGC
NgoMIV 327 GCCGGC G^CCGGC
NotI 844 GCGGCCGC GC^GGCCGC
PaeR7I 624 CTCGAG C^TCGAG
PflFI 2270 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2457 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2240 CACGTG CAC^GTG
PmlI 2240 CACGTG CAC^GTG
RcaI 643 TCATGA T^CATGA
SacI 2937 GAGCTC GAGCT^C
SbfI 130 CCTGCAGG CCTGCA^GG
SexAI 357 ACCTGGT A^CC[AT]GGT
SmaI 2035 CCCGGG CCC^GGG
Sse8387I 130 CCTGCAGG CCTGCA^GG
Tth111I 2270 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2457 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 624 CTCGAG C^TCGAG
XmaCI 2035 CCCGGG C^CCGGG
XmaI 2035 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 752 GTATAC GT^[AC][GT]AC
3130 GTCTAC
AcsI 1480 GAATTC [GA]^AATT[CT]
3743 GAATTC
AflIII 2898 ACATGT A^C[GA][CT]GT
4074 ACATGT
ApaI 903 GGGCCC GGGCC^C
1490 GGGCCC
ApoI 1480 GAATTC [GA]^AATT[CT]
3743 GAATTC
AspHI 350 GAGCAC G[AT]GC[AT]^C
2937 GAGCTC
BsiHKAI 350 GAGCAC G[AT]GC[AT]^C
2937 GAGCTC
BsmI 1611 GAATGC GAATGC(1/-1)
166 GCATTC
BspLU11I 2898 ACATGT A^CATGT
4074 ACATGT
DraIII 788 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
2237 CACCACGTG
EcoRI 1480 GAATTC G^AATTC
3743 GAATTC
HaeII 1339 GGCGCT [GA]GCGC^[CT]
2406 AGCGCT
HgaI 14 GACGC GACGC(5/10)
1679 GACGC
HindIII 1279 AAGCTT A^AGCTT
1691 AAGCTT
NcoI 1131 CCATGG C^CATGG
2091 CCATGG
NspI 2898 ACATGT [GA]CATG^[CT]
4074 ACATGT
PshAI 659 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2760 GACACTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII