##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI Asp700I AspEI
BamHI BbeI BbrPI BfrI BsaAI
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrDI BssHII BstBI
BstEII BstPI ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII KasI KspI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SwaI TaqI TthHB8I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1317 GTATAC GT^[AC][GT]AC
AcyI 2823 GGCGTC G[GA]^CG[CT]C
Alw44I 1398 GTGCAC G^TGCAC
AocI 1835 CCTCAGG CC^T[ACGT]AGG
ApaLI 1398 GTGCAC G^TGCAC
AseI 3911 ATTAAT AT^TAAT
AsnI 3911 ATTAAT AT^TAAT
AspI 193 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2458 TGCGCA TGC^GCA
AvrII 3764 CCTAGG C^CTAGG
BbvCI 289 GCTGAGG CCTCAGC(-5/-2)
BcgI 1627 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 618 GTATCC GTATCC(6/5)
BclI 1390 TGATCA T^GATCA
BglII 1981 AGATCT A^GATCT
BlnI 3764 CCTAGG C^CTAGG
BlpI 727 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 727 GCTGAGC GC^T[ACGT]AGC
BsaHI 2823 GGCGTC G[GA]^CG[CT]C
BsaI 68 GGTCTC GGTCTC(1/5)
BsaWI 12 ACCGGA [AT]^CCGG[AT]
BsmI 3668 GAATGC GAATGC(1/-1)
BsrFI 2820 GCCGGC [GA]^CCGG[CT]
BssSI 1625 CACGAG CACGAG(-5/-1)
Bst1107I 1317 GTATAC GTA^TAC
BstZ17I 1317 GTATAC GTA^TAC
Bsu36I 1835 CCTCAGG CC^T[ACGT]AGG
CelII 727 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2820 GCCGGC [GA]^CCGG[CT]
DraIII 1272 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1835 CCTCAGG CC^T[ACGT]AGG
EcoNI 3527 CCTGGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1390 TGATCA T^GATCA
FspI 2458 TGCGCA TGC^GCA
HgaI 2824 GCGTC GACGC(5/10)
Hin1I 2823 GGCGTC G[GA]^CG[CT]C
HincII 8 GTTAAC GT[CT]^[GA]AC
HindII 8 GTTAAC GT[CT]^[GA]AC
HpaI 8 GTTAAC GTT^AAC
NaeI 2820 GCCGGC GCC^GGC
NgoMIV 2820 GCCGGC G^CCGGC
PflFI 193 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3911 ATTAAT AT^TAAT
SacI 1536 GAGCTC GAGCT^C
SapI 577 GCTCTTC GCTCTTC(1/4)
ScaI 2238 AGTACT AGT^ACT
SfiI 2533 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2819 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 2889 CTTGAG C^T[CT][GA]AG
SspI 3855 AATATT AAT^ATT
Tth111I 193 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 32 CGCG CG^CG
51 CGCG
AflIII 988 ACGTGT A^C[GA][CT]GT
2909 ACACGT
ApaI 1029 GGGCCC GGGCC^C
2532 GGGCCC
Bsp1407I 222 TGTACA T^GTACA
1366 TGTACA
BspMI 1662 ACCTGC ACCTGC(4/8)
3693 GCAGGT
BsrBI 16 GAGCGG CCGCTC(-3/-3)
2424 GAGCGG
BsrGI 222 TGTACA T^GTACA
1366 TGTACA
BstUI 32 CGCG CG^CG
51 CGCG
BstXI 356 CCAAATGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1429 CCATGTACCTGG
BstYI 1981 AGATCT [GA]^GATC[CT]
2987 GGATCT
MaeII 988 ACGT A^CGT
2911 ACGT
MflI 1981 AGATCT [GA]^GATC[CT]
2987 GGATCT
MvnI 32 CGCG CG^CG
51 CGCG
NcoI 1237 CCATGG C^CATGG
3053 CCATGG
SspBI 222 TGTACA T^GTACA
1366 TGTACA
XcmI 201 CCAGCCCCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3053 CCATGGAGATACTGG
XhoII 1981 AGATCT [GA]^GATC[CT]
2987 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I