################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Aor51HI AscI Asp700I AspEI BamHI BbeI BbrPI BfrI BsaAI BsaBI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrDI BssHII BstBI BstEII BstPI ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI HindIII KasI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SwaI TaqI TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 1317 GTATAC GT^[AC][GT]AC AcyI 2823 GGCGTC G[GA]^CG[CT]C Alw44I 1398 GTGCAC G^TGCAC AocI 1835 CCTCAGG CC^T[ACGT]AGG ApaLI 1398 GTGCAC G^TGCAC AseI 3911 ATTAAT AT^TAAT AsnI 3911 ATTAAT AT^TAAT AspI 193 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2458 TGCGCA TGC^GCA AvrII 3764 CCTAGG C^CTAGG BbvCI 289 GCTGAGG CCTCAGC(-5/-2) BcgI 1627 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 618 GTATCC GTATCC(6/5) BclI 1390 TGATCA T^GATCA BglII 1981 AGATCT A^GATCT BlnI 3764 CCTAGG C^CTAGG BlpI 727 GCTGAGC GC^T[ACGT]AGC Bpu1102I 727 GCTGAGC GC^T[ACGT]AGC BsaHI 2823 GGCGTC G[GA]^CG[CT]C BsaI 68 GGTCTC GGTCTC(1/5) BsaWI 12 ACCGGA [AT]^CCGG[AT] BsmI 3668 GAATGC GAATGC(1/-1) BsrFI 2820 GCCGGC [GA]^CCGG[CT] BssSI 1625 CACGAG CACGAG(-5/-1) Bst1107I 1317 GTATAC GTA^TAC BstZ17I 1317 GTATAC GTA^TAC Bsu36I 1835 CCTCAGG CC^T[ACGT]AGG CelII 727 GCTGAGC GC^T[ACGT]AGC Cfr10I 2820 GCCGGC [GA]^CCGG[CT] DraIII 1272 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1835 CCTCAGG CC^T[ACGT]AGG EcoNI 3527 CCTGGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1390 TGATCA T^GATCA FspI 2458 TGCGCA TGC^GCA HgaI 2824 GCGTC GACGC(5/10) Hin1I 2823 GGCGTC G[GA]^CG[CT]C HincII 8 GTTAAC GT[CT]^[GA]AC HindII 8 GTTAAC GT[CT]^[GA]AC HpaI 8 GTTAAC GTT^AAC NaeI 2820 GCCGGC GCC^GGC NgoMIV 2820 GCCGGC G^CCGGC PflFI 193 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 3911 ATTAAT AT^TAAT SacI 1536 GAGCTC GAGCT^C SapI 577 GCTCTTC GCTCTTC(1/4) ScaI 2238 AGTACT AGT^ACT SfiI 2533 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 2819 CGCCGGCG C[GA]^CCGG[CT]G SmlI 2889 CTTGAG C^T[CT][GA]AG SspI 3855 AATATT AAT^ATT Tth111I 193 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 32 CGCG CG^CG 51 CGCG AflIII 988 ACGTGT A^C[GA][CT]GT 2909 ACACGT ApaI 1029 GGGCCC GGGCC^C 2532 GGGCCC Bsp1407I 222 TGTACA T^GTACA 1366 TGTACA BspMI 1662 ACCTGC ACCTGC(4/8) 3693 GCAGGT BsrBI 16 GAGCGG CCGCTC(-3/-3) 2424 GAGCGG BsrGI 222 TGTACA T^GTACA 1366 TGTACA BstUI 32 CGCG CG^CG 51 CGCG BstXI 356 CCAAATGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1429 CCATGTACCTGG BstYI 1981 AGATCT [GA]^GATC[CT] 2987 GGATCT MaeII 988 ACGT A^CGT 2911 ACGT MflI 1981 AGATCT [GA]^GATC[CT] 2987 GGATCT MvnI 32 CGCG CG^CG 51 CGCG NcoI 1237 CCATGG C^CATGG 3053 CCATGG SspBI 222 TGTACA T^GTACA 1366 TGTACA XcmI 201 CCAGCCCCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3053 CCATGGAGATACTGG XhoII 1981 AGATCT [GA]^GATC[CT] 2987 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### Acc65I AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I