################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AccIII AclI AflII AhdI Aor51HI AscI AseI AsnI AspEI AviII BamHI BbeI BbvCI BciVI BfrI BlpI Bpu1102I BseAI BsiEI BsiWI BsmI BspDI BspEI BsrBI BssHII BstEII BstPI CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FseI FspI HaeII HpaI KasI KspI MluI MroI NaeI NarI NcoI NgoMIV NotI NruI PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI ScaI SexAI SfoI SgrAI SphI SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1997 GACGTC GACGT^C AflIII 2026 ACATGT A^C[GA][CT]GT AgeI 2498 ACCGGT A^CCGGT AocI 895 CCTCAGG CC^T[ACGT]AGG ApaI 623 GGGCCC GGGCC^C AvaI 1073 CCCGGG C^[CT]CG[GA]G AvrII 3493 CCTAGG C^CTAGG BanII 623 GGGCCC G[GA]GC[CT]^C BbrPI 2910 CACGTG CAC^GTG BbsI 1489 GTCTTC GAAGAC(2/6) BcgI 2951 CGATGCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 3493 CCTAGG C^CTAGG BpuAI 1489 GTCTTC GAAGAC(2/6) BsoBI 1073 CCCGGG C^[CT]CG[GA]G BspLU11I 2026 ACATGT A^CATGT BstBI 1475 TTCGAA TT^CGAA Bsu36I 895 CCTCAGG CC^T[ACGT]AGG DrdI 2535 GACTAAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 895 CCTCAGG CC^T[ACGT]AGG EcoRI 414 GAATTC G^AATTC EcoRV 4125 GATATC GAT^ATC EcoT22I 1883 ATGCAT ATGCA^T NheI 390 GCTAGC G^CTAGC NsiI 1883 ATGCAT ATGCA^T NspV 1475 TTCGAA TT^CGAA PinAI 2498 ACCGGT A^CCGGT PmaCI 2910 CACGTG CAC^GTG PmlI 2910 CACGTG CAC^GTG PpuMI 2228 AGGACCC [GA]G^G[AT]CC[CT] PvuII 192 CAGCTG CAG^CTG SbfI 1956 CCTGCAGG CCTGCA^GG SfiI 2406 GGCCACGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1475 TTCGAA TT^CGAA SmaI 1073 CCCGGG CCC^GGG SmlI 90 CTTGAG C^T[CT][GA]AG SnaBI 3317 TACGTA TAC^GTA Sse8387I 1956 CCTGCAGG CCTGCA^GG StuI 931 AGGCCT AGG^CCT XbaI 3731 TCTAGA T^CTAGA XmaCI 1073 CCCGGG C^CCGGG XmaI 1073 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 545 GGTACC G^GTACC 2381 GGTACC AccII 14 CGCG CG^CG 39 CGCG AcyI 1213 GACGCC G[GA]^CG[CT]C 1997 GACGTC Alw44I 355 GTGCAC G^TGCAC 3597 GTGCAC AlwI 448 GATCC GGATC(4/5) 1367 GGATC AlwNI 107 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 3335 CAGCTTCTG ApaLI 355 GTGCAC G^TGCAC 3597 GTGCAC Asp718I 545 GGTACC G^GTACC 2381 GGTACC AspI 64 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1974 GACCCTGTC BanI 545 GGTACC G^G[CT][GA]CC 2381 GGTACC BglII 3296 AGATCT A^GATCT 3802 AGATCT BsaAI 2910 CACGTG [CT]AC^GT[GA] 3317 TACGTA BsaBI 750 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 850 GATGGAAATC BsaHI 1213 GACGCC G[GA]^CG[CT]C 1997 GACGTC BsaI 317 GAGACC GGTCTC(1/5) 1673 GAGACC BsaWI 123 ACCGGA [AT]^CCGG[AT] 2498 ACCGGT BseRI 1851 CTCCTC GAGGAG(10/8) 1414 GAGGAG BsmBI 10 GAGACG CGTCTC(1/5) 2290 GAGACG BspHI 1596 TCATGA T^CATGA 4090 TCATGA BsrFI 2326 ACCGGC [GA]^CCGG[CT] 2498 ACCGGT BssSI 951 CACGAG CACGAG(-5/-1) 2106 CACGAG Bst1107I 2726 GTATAC GTA^TAC 3343 GTATAC BstUI 14 CGCG CG^CG 39 CGCG BstYI 3296 AGATCT [GA]^GATC[CT] 3802 AGATCT BstZ17I 2726 GTATAC GTA^TAC 3343 GTATAC Cfr10I 2326 ACCGGC [GA]^CCGG[CT] 2498 ACCGGT EcoNI 531 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3488 CCTAGCCTAGG EcoT14I 3493 CCTAGG C^C[AT][AT]GG 3845 CCTTGG Hin1I 1213 GACGCC G[GA]^CG[CT]C 1997 GACGTC KpnI 545 GGTACC GGTAC^C 2381 GGTACC MamI 750 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 850 GATGGAAATC MfeI 1989 CAATTG C^AATTG 3546 CAATTG MflI 3296 AGATCT [GA]^GATC[CT] 3802 AGATCT MspA1I 192 CAGCTG C[AC]G^C[GT]G 1363 CAGCGG MunI 1989 CAATTG C^AATTG 3546 CAATTG MvnI 14 CGCG CG^CG 39 CGCG NdeI 2860 CATATG CA^TATG 3348 CATATG PflFI 64 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1974 GACCCTGTC PflMI 225 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1510 CCATATAGTGG RcaI 1596 TCATGA T^CATGA 4090 TCATGA SapI 499 GAAGAGC GCTCTTC(1/4) 2926 GCTCTTC SpeI 1644 ACTAGT A^CTAGT 2888 ACTAGT SspI 3078 AATATT AAT^ATT 3113 AATATT StyI 3493 CCTAGG C^C[AT][AT]GG 3845 CCTTGG Tth111I 64 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1974 GACCCTGTC Van91I 225 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1510 CCATATAGTGG XhoII 3296 AGATCT [GA]^GATC[CT] 3802 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccI AciI AcsI AfaI AluI ApoI Asp700I AspHI AvaII BalI BbvI BclI BcnI BfaI BglI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI