##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AclI AflII AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BamHI BbeI BbvCI BciVI BfrI
BlpI Bpu1102I BseAI BsiEI BsiWI
BsmI BspDI BspEI BsrBI BssHII
BstEII BstPI CelII ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI HaeII
HpaI KasI KspI MluI MroI
NaeI NarI NcoI NgoMIV NotI
NruI PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI ScaI SexAI SfoI
SgrAI SphI SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1997 GACGTC GACGT^C
AflIII 2026 ACATGT A^C[GA][CT]GT
AgeI 2498 ACCGGT A^CCGGT
AocI 895 CCTCAGG CC^T[ACGT]AGG
ApaI 623 GGGCCC GGGCC^C
AvaI 1073 CCCGGG C^[CT]CG[GA]G
AvrII 3493 CCTAGG C^CTAGG
BanII 623 GGGCCC G[GA]GC[CT]^C
BbrPI 2910 CACGTG CAC^GTG
BbsI 1489 GTCTTC GAAGAC(2/6)
BcgI 2951 CGATGCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3493 CCTAGG C^CTAGG
BpuAI 1489 GTCTTC GAAGAC(2/6)
BsoBI 1073 CCCGGG C^[CT]CG[GA]G
BspLU11I 2026 ACATGT A^CATGT
BstBI 1475 TTCGAA TT^CGAA
Bsu36I 895 CCTCAGG CC^T[ACGT]AGG
DrdI 2535 GACTAAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 895 CCTCAGG CC^T[ACGT]AGG
EcoRI 414 GAATTC G^AATTC
EcoRV 4125 GATATC GAT^ATC
EcoT22I 1883 ATGCAT ATGCA^T
NheI 390 GCTAGC G^CTAGC
NsiI 1883 ATGCAT ATGCA^T
NspV 1475 TTCGAA TT^CGAA
PinAI 2498 ACCGGT A^CCGGT
PmaCI 2910 CACGTG CAC^GTG
PmlI 2910 CACGTG CAC^GTG
PpuMI 2228 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 192 CAGCTG CAG^CTG
SbfI 1956 CCTGCAGG CCTGCA^GG
SfiI 2406 GGCCACGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1475 TTCGAA TT^CGAA
SmaI 1073 CCCGGG CCC^GGG
SmlI 90 CTTGAG C^T[CT][GA]AG
SnaBI 3317 TACGTA TAC^GTA
Sse8387I 1956 CCTGCAGG CCTGCA^GG
StuI 931 AGGCCT AGG^CCT
XbaI 3731 TCTAGA T^CTAGA
XmaCI 1073 CCCGGG C^CCGGG
XmaI 1073 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 545 GGTACC G^GTACC
2381 GGTACC
AccII 14 CGCG CG^CG
39 CGCG
AcyI 1213 GACGCC G[GA]^CG[CT]C
1997 GACGTC
Alw44I 355 GTGCAC G^TGCAC
3597 GTGCAC
AlwI 448 GATCC GGATC(4/5)
1367 GGATC
AlwNI 107 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
3335 CAGCTTCTG
ApaLI 355 GTGCAC G^TGCAC
3597 GTGCAC
Asp718I 545 GGTACC G^GTACC
2381 GGTACC
AspI 64 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1974 GACCCTGTC
BanI 545 GGTACC G^G[CT][GA]CC
2381 GGTACC
BglII 3296 AGATCT A^GATCT
3802 AGATCT
BsaAI 2910 CACGTG [CT]AC^GT[GA]
3317 TACGTA
BsaBI 750 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
850 GATGGAAATC
BsaHI 1213 GACGCC G[GA]^CG[CT]C
1997 GACGTC
BsaI 317 GAGACC GGTCTC(1/5)
1673 GAGACC
BsaWI 123 ACCGGA [AT]^CCGG[AT]
2498 ACCGGT
BseRI 1851 CTCCTC GAGGAG(10/8)
1414 GAGGAG
BsmBI 10 GAGACG CGTCTC(1/5)
2290 GAGACG
BspHI 1596 TCATGA T^CATGA
4090 TCATGA
BsrFI 2326 ACCGGC [GA]^CCGG[CT]
2498 ACCGGT
BssSI 951 CACGAG CACGAG(-5/-1)
2106 CACGAG
Bst1107I 2726 GTATAC GTA^TAC
3343 GTATAC
BstUI 14 CGCG CG^CG
39 CGCG
BstYI 3296 AGATCT [GA]^GATC[CT]
3802 AGATCT
BstZ17I 2726 GTATAC GTA^TAC
3343 GTATAC
Cfr10I 2326 ACCGGC [GA]^CCGG[CT]
2498 ACCGGT
EcoNI 531 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3488 CCTAGCCTAGG
EcoT14I 3493 CCTAGG C^C[AT][AT]GG
3845 CCTTGG
Hin1I 1213 GACGCC G[GA]^CG[CT]C
1997 GACGTC
KpnI 545 GGTACC GGTAC^C
2381 GGTACC
MamI 750 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
850 GATGGAAATC
MfeI 1989 CAATTG C^AATTG
3546 CAATTG
MflI 3296 AGATCT [GA]^GATC[CT]
3802 AGATCT
MspA1I 192 CAGCTG C[AC]G^C[GT]G
1363 CAGCGG
MunI 1989 CAATTG C^AATTG
3546 CAATTG
MvnI 14 CGCG CG^CG
39 CGCG
NdeI 2860 CATATG CA^TATG
3348 CATATG
PflFI 64 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1974 GACCCTGTC
PflMI 225 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1510 CCATATAGTGG
RcaI 1596 TCATGA T^CATGA
4090 TCATGA
SapI 499 GAAGAGC GCTCTTC(1/4)
2926 GCTCTTC
SpeI 1644 ACTAGT A^CTAGT
2888 ACTAGT
SspI 3078 AATATT AAT^ATT
3113 AATATT
StyI 3493 CCTAGG C^C[AT][AT]GG
3845 CCTTGG
Tth111I 64 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1974 GACCCTGTC
Van91I 225 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1510 CCATATAGTGG
XhoII 3296 AGATCT [GA]^GATC[CT]
3802 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI Asp700I AspHI AvaII BalI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI