################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccI AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AvrII BamHI BcgI BciVI BclI BfrI BlnI BsiEI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I ClaI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KspI MluI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 3828 GGTACC G^GTACC AccII 1763 CGCG CG^CG AccIII 2773 TCCGGA T^CCGGA AflIII 3941 ACACGT A^C[GA][CT]GT Asp700I 221 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3828 GGTACC G^GTACC AviII 1235 TGCGCA TGC^GCA BalI 889 TGGCCA TGG^CCA BbrPI 1974 CACGTG CAC^GTG BglI 2224 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1597 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2773 TCCGGA [AT]^CCGG[AT] BseAI 2773 TCCGGA T^CCGGA BsgI 2988 CTGCAC GTGCAG(16/14) BsiWI 4073 CGTACG C^GTACG BspEI 2773 TCCGGA T^CCGGA BstAPI 254 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1763 CGCG CG^CG CpoI 2775 CGGACCG CG^G[AT]CCG DraI 617 TTTAAA TTT^AAA DrdI 1314 GACGAGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 2140 CCATGG C^C[GA][CT]GG FspI 1235 TGCGCA TGC^GCA HincII 1718 GTCAAC GT[CT]^[GA]AC HindII 1718 GTCAAC GT[CT]^[GA]AC KpnI 3828 GGTACC GGTAC^C MamI 1597 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2758 CAATTG C^AATTG MluNI 889 TGGCCA TGG^CCA MroI 2773 TCCGGA T^CCGGA MscI 889 TGGCCA TGG^CCA MslI 2457 CATGCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 2758 CAATTG C^AATTG MvnI 1763 CGCG CG^CG NaeI 2627 GCCGGC GCC^GGC NcoI 2140 CCATGG C^CATGG NgoMIV 2627 GCCGGC G^CCGGC NheI 3799 GCTAGC G^CTAGC PmaCI 1974 CACGTG CAC^GTG PmlI 1974 CACGTG CAC^GTG RsrII 2775 CGGACCG CG^G[AT]CCG SexAI 2836 ACCTGGT A^CC[AT]GGT SgrAI 2626 CGCCGGCG C[GA]^CCGG[CT]G SmaI 3175 CCCGGG CCC^GGG XcmI 2732 CCAACAGAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 3175 CCCGGG C^CCGGG XmaI 3175 CCCGGG C^CCGGG XmnI 221 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 2630 GGCGCC G[GA]^CG[CT]C 3793 GGCGCC AocI 348 CCTGAGG CC^T[ACGT]AGG 593 CCTGAGG AvaI 289 CTCGGG C^[CT]CG[GA]G 3175 CCCGGG BbeI 2630 GGCGCC GGCGC^C 3793 GGCGCC BbsI 1011 GAAGAC GAAGAC(2/6) 956 GTCTTC BglII 1596 AGATCT A^GATCT 2077 AGATCT BpuAI 1011 GAAGAC GAAGAC(2/6) 956 GTCTTC BsaAI 1974 CACGTG [CT]AC^GT[GA] 4075 TACGTG BsaHI 2630 GGCGCC G[GA]^CG[CT]C 3793 GGCGCC BsmBI 1222 CGTCTC CGTCTC(1/5) 1636 CGTCTC BsmI 1356 GAATGC GAATGC(1/-1) 180 GCATTC BsoBI 289 CTCGGG C^[CT]CG[GA]G 3175 CCCGGG BsrFI 2627 GCCGGC [GA]^CCGG[CT] 3598 ACCGGC BssSI 1672 CACGAG CACGAG(-5/-1) 1692 CACGAG Bsu36I 348 CCTGAGG CC^T[ACGT]AGG 593 CCTGAGG Cfr10I 2627 GCCGGC [GA]^CCGG[CT] 3598 ACCGGC Eco81I 348 CCTGAGG CC^T[ACGT]AGG 593 CCTGAGG Hin1I 2630 GGCGCC G[GA]^CG[CT]C 3793 GGCGCC KasI 2630 GGCGCC G^GCGCC 3793 GGCGCC NarI 2630 GGCGCC GG^CGCC 3793 GGCGCC SacI 411 GAGCTC GAGCT^C 437 GAGCTC SapI 1495 GAAGAGC GCTCTTC(1/4) 4042 GAAGAGC SfoI 2630 GGCGCC GGC^GCC 3793 GGCGCC SmlI 934 CTCAAG C^T[CT][GA]AG 2433 CTCAAG StuI 83 AGGCCT AGG^CCT 2905 AGGCCT XbaI 585 TCTAGA T^CTAGA 705 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaII BanI BanII BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII