##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AvrII
BamHI BcgI BciVI BclI BfrI
BlnI BsiEI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KspI MluI NdeI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI SacII SalI
SbfI ScaI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3828 GGTACC G^GTACC
AccII 1763 CGCG CG^CG
AccIII 2773 TCCGGA T^CCGGA
AflIII 3941 ACACGT A^C[GA][CT]GT
Asp700I 221 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3828 GGTACC G^GTACC
AviII 1235 TGCGCA TGC^GCA
BalI 889 TGGCCA TGG^CCA
BbrPI 1974 CACGTG CAC^GTG
BglI 2224 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1597 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2773 TCCGGA [AT]^CCGG[AT]
BseAI 2773 TCCGGA T^CCGGA
BsgI 2988 CTGCAC GTGCAG(16/14)
BsiWI 4073 CGTACG C^GTACG
BspEI 2773 TCCGGA T^CCGGA
BstAPI 254 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1763 CGCG CG^CG
CpoI 2775 CGGACCG CG^G[AT]CCG
DraI 617 TTTAAA TTT^AAA
DrdI 1314 GACGAGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2140 CCATGG C^C[GA][CT]GG
FspI 1235 TGCGCA TGC^GCA
HincII 1718 GTCAAC GT[CT]^[GA]AC
HindII 1718 GTCAAC GT[CT]^[GA]AC
KpnI 3828 GGTACC GGTAC^C
MamI 1597 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2758 CAATTG C^AATTG
MluNI 889 TGGCCA TGG^CCA
MroI 2773 TCCGGA T^CCGGA
MscI 889 TGGCCA TGG^CCA
MslI 2457 CATGCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2758 CAATTG C^AATTG
MvnI 1763 CGCG CG^CG
NaeI 2627 GCCGGC GCC^GGC
NcoI 2140 CCATGG C^CATGG
NgoMIV 2627 GCCGGC G^CCGGC
NheI 3799 GCTAGC G^CTAGC
PmaCI 1974 CACGTG CAC^GTG
PmlI 1974 CACGTG CAC^GTG
RsrII 2775 CGGACCG CG^G[AT]CCG
SexAI 2836 ACCTGGT A^CC[AT]GGT
SgrAI 2626 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3175 CCCGGG CCC^GGG
XcmI 2732 CCAACAGAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3175 CCCGGG C^CCGGG
XmaI 3175 CCCGGG C^CCGGG
XmnI 221 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 2630 GGCGCC G[GA]^CG[CT]C
3793 GGCGCC
AocI 348 CCTGAGG CC^T[ACGT]AGG
593 CCTGAGG
AvaI 289 CTCGGG C^[CT]CG[GA]G
3175 CCCGGG
BbeI 2630 GGCGCC GGCGC^C
3793 GGCGCC
BbsI 1011 GAAGAC GAAGAC(2/6)
956 GTCTTC
BglII 1596 AGATCT A^GATCT
2077 AGATCT
BpuAI 1011 GAAGAC GAAGAC(2/6)
956 GTCTTC
BsaAI 1974 CACGTG [CT]AC^GT[GA]
4075 TACGTG
BsaHI 2630 GGCGCC G[GA]^CG[CT]C
3793 GGCGCC
BsmBI 1222 CGTCTC CGTCTC(1/5)
1636 CGTCTC
BsmI 1356 GAATGC GAATGC(1/-1)
180 GCATTC
BsoBI 289 CTCGGG C^[CT]CG[GA]G
3175 CCCGGG
BsrFI 2627 GCCGGC [GA]^CCGG[CT]
3598 ACCGGC
BssSI 1672 CACGAG CACGAG(-5/-1)
1692 CACGAG
Bsu36I 348 CCTGAGG CC^T[ACGT]AGG
593 CCTGAGG
Cfr10I 2627 GCCGGC [GA]^CCGG[CT]
3598 ACCGGC
Eco81I 348 CCTGAGG CC^T[ACGT]AGG
593 CCTGAGG
Hin1I 2630 GGCGCC G[GA]^CG[CT]C
3793 GGCGCC
KasI 2630 GGCGCC G^GCGCC
3793 GGCGCC
NarI 2630 GGCGCC GG^CGCC
3793 GGCGCC
SacI 411 GAGCTC GAGCT^C
437 GAGCTC
SapI 1495 GAAGAGC GCTCTTC(1/4)
4042 GAAGAGC
SfoI 2630 GGCGCC GGC^GCC
3793 GGCGCC
SmlI 934 CTCAAG C^T[CT][GA]AG
2433 CTCAAG
StuI 83 AGGCCT AGG^CCT
2905 AGGCCT
XbaI 585 TCTAGA T^CTAGA
705 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AspI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflFI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII