##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AlwNI AocI Aor51HI ApaI
AscI Asp718I AvaI AviII BamHI
BbeI BbrPI BcgI BciVI BglI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI Hin1I KasI KpnI
KspI MamI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1308 GTCTAC GT^[AC][GT]AC
AccII 34 CGCG CG^CG
AflII 2804 CTTAAG C^TTAAG
AflIII 3676 ACATGT A^C[GA][CT]GT
AhdI 2115 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 479 GTGCAC G^TGCAC
ApaLI 479 GTGCAC G^TGCAC
AspEI 2115 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1028 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 787 CCTAGG C^CTAGG
BanI 2494 GGTGCC G^G[CT][GA]CC
BbvCI 2128 GCTGAGG CCTCAGC(-5/-2)
BfrI 2804 CTTAAG C^TTAAG
BlnI 787 CCTAGG C^CTAGG
BlpI 1816 GCTGAGC GC^T[ACGT]AGC
BpmI 2060 CTCCAG CTGGAG(16/14)
Bpu1102I 1816 GCTGAGC GC^T[ACGT]AGC
BsmI 2511 GAATGC GAATGC(1/-1)
BspLU11I 3676 ACATGT A^CATGT
BsrBI 40 CCGCTC CCGCTC(-3/-3)
BstAPI 415 GCACGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 34 CGCG CG^CG
CelII 1816 GCTGAGC GC^T[ACGT]AGC
DraII 723 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 1795 GACAGTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2115 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 723 AGGCCCT [GA]G^G[ACGT]CC[CT]
MaeII 1029 ACGT A^CGT
MvnI 34 CGCG CG^CG
PflFI 1028 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1369 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 470 GAGCTC GAGCT^C
SphI 2210 GCATGC GCATG^C
StuI 345 AGGCCT AGG^CCT
TaqI 1218 TCGA T^CGA
Tth111I 1028 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1218 TCGA T^CGA
Van91I 1369 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 590 CCAGTAATATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AseI 1567 ATTAAT AT^TAAT
3488 ATTAAT
AsnI 1567 ATTAAT AT^TAAT
3488 ATTAAT
Asp700I 443 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
881 GAAGTCGTTC
BanII 470 GAGCTC G[GA]GC[CT]^C
2203 GAGCCC
BclI 1731 TGATCA T^GATCA
2433 TGATCA
BcnI 2042 CCCGG CC^[GC]GG
2491 CCGGG
BglII 754 AGATCT A^GATCT
1054 AGATCT
BsaI 979 GAGACC GGTCTC(1/5)
2296 GAGACC
BseRI 1082 CTCCTC GAGGAG(10/8)
708 GAGGAG
Bsp1407I 2811 TGTACA T^GTACA
2902 TGTACA
BspMI 3670 ACCTGC ACCTGC(4/8)
3321 GCAGGT
BsrGI 2811 TGTACA T^GTACA
2902 TGTACA
CfoI 2 GCGC GCG^C
1079 GCGC
EarI 821 GAAGAG CTCTTC(1/4)
2146 GAAGAG
EcoNI 2005 CCTAACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2653 CCTAAAACAGG
FauI 39 CCCGC CCCGC(4/6)
13 GCGGG
FbaI 1731 TGATCA T^GATCA
2433 TGATCA
HaeII 1 AGCGCC [GA]GCGC^[CT]
1078 GGCGCT
HapII 2043 CCGG C^CGG
2491 CCGG
HgaI 3648 GACGC GACGC(5/10)
35 GCGTC
HhaI 2 GCGC GCG^C
1079 GCGC
HinP1I 2 GCGC G^CGC
1079 GCGC
HindIII 1810 AAGCTT A^AGCTT
2819 AAGCTT
HpaI 172 GTTAAC GTT^AAC
2859 GTTAAC
HpaII 2043 CCGG C^CGG
2491 CCGG
Ksp632I 821 GAAGAG CTCTTC(1/4)
2146 GAAGAG
MfeI 3178 CAATTG C^AATTG
3316 CAATTG
MspA1I 1507 CAGCTG C[AC]G^C[GT]G
2126 CAGCTG
MspI 2043 CCGG C^CGG
2491 CCGG
MunI 3178 CAATTG C^AATTG
3316 CAATTG
NciI 2042 CCCGG CC^[GC]GG
2491 CCGGG
NcoI 876 CCATGG C^CATGG
1110 CCATGG
PshBI 1567 ATTAAT AT^TAAT
3488 ATTAAT
PvuII 1507 CAGCTG CAG^CTG
2126 CAGCTG
ScaI 496 AGTACT AGT^ACT
3025 AGTACT
SspBI 2811 TGTACA T^GTACA
2902 TGTACA
XmnI 443 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
881 GAAGTCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII