##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

Acc65I      AccIII      AflIII      AhdI        AocI        
AscI        Asp700I     Asp718I     AspEI       AviII       
BamHI       BcgI        BglII       BsaBI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssSI       Bst1107I    
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DrdI        Eam1105I    Eco81I      EcoO65I     
EcoRI       EcoT22I     FspI        HindIII     HpaI        
KpnI        KspI        MamI        MluI        MroI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflMI       PmeI        PvuI        RcaI        RsrII       
SacII       SalI        SexAI       SfiI        SgrAI       
SnaBI       SpeI        SspBI       SwaI        Van91I      
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII         249  GACGTC               GACGT^C
AccI         2012  GTAGAC               GT^[AC][GT]AC
AclI         2428  AACGTT               AA^CGTT
AcsI         2046  GAATTT               [GA]^AATT[CT]
AgeI         1609  ACCGGT               A^CCGGT
Alw44I       3881  GTGCAC               G^TGCAC
ApaLI        3881  GTGCAC               G^TGCAC
ApoI         2046  GAATTT               [GA]^AATT[CT]
AseI         3978  ATTAAT               AT^TAAT
AsnI         3978  ATTAAT               AT^TAAT
AspI         3913  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1726  CCCGGG               C^[CT]CG[GA]G
AvrII        2462  CCTAGG               C^CTAGG
BbvCI         976  CCTCAGC              CCTCAGC(-5/-2)
BciVI         232  GGATAC               GTATCC(6/5)
BclI         1372  TGATCA               T^GATCA
BlnI         2462  CCTAGG               C^CTAGG
BsaWI        1609  ACCGGT               [AT]^CCGG[AT]
BsmBI        3145  GAGACG               CGTCTC(1/5)
BsoBI        1726  CCCGGG               C^[CT]CG[GA]G
BsrBI         808  GAGCGG               CCGCTC(-3/-3)
BssHII       1445  GCGCGC               G^CGCGC
BstAPI       1382  GCAGACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        3011  TTCGAA               TT^CGAA
DraIII       1331  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        2373  CCTGATGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV          97  GATATC               GAT^ATC
FbaI         1372  TGATCA               T^GATCA
FseI         1755  GGCCGGCC             GGCCGG^CC
HincII       3747  GTTGAC               GT[CT]^[GA]AC
HindII       3747  GTTGAC               GT[CT]^[GA]AC
MfeI          288  CAATTG               C^AATTG
MunI          288  CAATTG               C^AATTG
NaeI         1756  GCCGGC               GCC^GGC
NdeI         1050  CATATG               CA^TATG
NgoMIV       1756  GCCGGC               G^CCGGC
NheI         2647  GCTAGC               G^CTAGC
NspV         3011  TTCGAA               TT^CGAA
PflFI        3913  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1609  ACCGGT               A^CCGGT
PshAI        3147  GACGCTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3978  ATTAAT               AT^TAAT
Psp1406I     2428  AACGTT               AA^CGTT
SapI         1966  GCTCTTC              GCTCTTC(1/4)
SbfI         1785  CCTGCAGG             CCTGCA^GG
ScaI         3857  AGTACT               AGT^ACT
SfuI         3011  TTCGAA               TT^CGAA
SmaI         1726  CCCGGG               CCC^GGG
Sse8387I     1785  CCTGCAGG             CCTGCA^GG
SspI         1655  AATATT               AAT^ATT
StuI         3377  AGGCCT               AGG^CCT
Tth111I      3913  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1726  CCCGGG               C^CCGGG
XmaI         1726  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

Aor51HI       497  AGCGCT               AGC^GCT
              727  AGCGCT              
ApaI          297  GGGCCC               GGGCC^C
             1200  GGGCCC              
BanI          173  GGCGCC               G^G[CT][GA]CC
             1815  GGCGCC              
BbeI          173  GGCGCC               GGCGC^C
             1815  GGCGCC              
EagI           71  CGGCCG               C^GGCCG
             1754  CGGCCG              
EclXI          71  CGGCCG               C^GGCCG
             1754  CGGCCG              
Eco47III      497  AGCGCT               AGC^GCT
              727  AGCGCT              
Eco52I         71  CGGCCG               C^GGCCG
             1754  CGGCCG              
KasI          173  GGCGCC               G^GCGCC
             1815  GGCGCC              
NarI          173  GGCGCC               GG^CGCC
             1815  GGCGCC              
SacI          276  GAGCTC               GAGCT^C
             1098  GAGCTC              
SfoI          173  GGCGCC               GGC^GCC
             1815  GGCGCC              
XbaI         2449  TCTAGA               T^CTAGA
             2942  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflII       
AluI        AlwI        AlwNI       AspHI       AvaII       
BalI        BanII       BbrPI       BbsI        BbvI        
BcnI        BfaI        BfrI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PmaCI       PmlI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII