################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AccIII AflIII AhdI AocI AscI Asp700I Asp718I AspEI AviII BamHI BcgI BglII BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI Eam1105I Eco81I EcoO65I EcoRI EcoT22I FspI HindIII HpaI KpnI KspI MamI MluI MroI NotI NruI NsiI PacI PaeR7I PflMI PmeI PvuI RcaI RsrII SacII SalI SexAI SfiI SgrAI SnaBI SpeI SspBI SwaI Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 249 GACGTC GACGT^C AccI 2012 GTAGAC GT^[AC][GT]AC AclI 2428 AACGTT AA^CGTT AcsI 2046 GAATTT [GA]^AATT[CT] AgeI 1609 ACCGGT A^CCGGT Alw44I 3881 GTGCAC G^TGCAC ApaLI 3881 GTGCAC G^TGCAC ApoI 2046 GAATTT [GA]^AATT[CT] AseI 3978 ATTAAT AT^TAAT AsnI 3978 ATTAAT AT^TAAT AspI 3913 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1726 CCCGGG C^[CT]CG[GA]G AvrII 2462 CCTAGG C^CTAGG BbvCI 976 CCTCAGC CCTCAGC(-5/-2) BciVI 232 GGATAC GTATCC(6/5) BclI 1372 TGATCA T^GATCA BlnI 2462 CCTAGG C^CTAGG BsaWI 1609 ACCGGT [AT]^CCGG[AT] BsmBI 3145 GAGACG CGTCTC(1/5) BsoBI 1726 CCCGGG C^[CT]CG[GA]G BsrBI 808 GAGCGG CCGCTC(-3/-3) BssHII 1445 GCGCGC G^CGCGC BstAPI 1382 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3011 TTCGAA TT^CGAA DraIII 1331 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 2373 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 97 GATATC GAT^ATC FbaI 1372 TGATCA T^GATCA FseI 1755 GGCCGGCC GGCCGG^CC HincII 3747 GTTGAC GT[CT]^[GA]AC HindII 3747 GTTGAC GT[CT]^[GA]AC MfeI 288 CAATTG C^AATTG MunI 288 CAATTG C^AATTG NaeI 1756 GCCGGC GCC^GGC NdeI 1050 CATATG CA^TATG NgoMIV 1756 GCCGGC G^CCGGC NheI 2647 GCTAGC G^CTAGC NspV 3011 TTCGAA TT^CGAA PflFI 3913 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1609 ACCGGT A^CCGGT PshAI 3147 GACGCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3978 ATTAAT AT^TAAT Psp1406I 2428 AACGTT AA^CGTT SapI 1966 GCTCTTC GCTCTTC(1/4) SbfI 1785 CCTGCAGG CCTGCA^GG ScaI 3857 AGTACT AGT^ACT SfuI 3011 TTCGAA TT^CGAA SmaI 1726 CCCGGG CCC^GGG Sse8387I 1785 CCTGCAGG CCTGCA^GG SspI 1655 AATATT AAT^ATT StuI 3377 AGGCCT AGG^CCT Tth111I 3913 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1726 CCCGGG C^CCGGG XmaI 1726 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### Aor51HI 497 AGCGCT AGC^GCT 727 AGCGCT ApaI 297 GGGCCC GGGCC^C 1200 GGGCCC BanI 173 GGCGCC G^G[CT][GA]CC 1815 GGCGCC BbeI 173 GGCGCC GGCGC^C 1815 GGCGCC EagI 71 CGGCCG C^GGCCG 1754 CGGCCG EclXI 71 CGGCCG C^GGCCG 1754 CGGCCG Eco47III 497 AGCGCT AGC^GCT 727 AGCGCT Eco52I 71 CGGCCG C^GGCCG 1754 CGGCCG KasI 173 GGCGCC G^GCGCC 1815 GGCGCC NarI 173 GGCGCC GG^CGCC 1815 GGCGCC SacI 276 GAGCTC GAGCT^C 1098 GAGCTC SfoI 173 GGCGCC GGC^GCC 1815 GGCGCC XbaI 2449 TCTAGA T^CTAGA 2942 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcyI AfaI AflII AluI AlwI AlwNI AspHI AvaII BalI BanII BbrPI BbsI BbvI BcnI BfaI BfrI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII