##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AflIII AhdI AocI
AscI Asp700I Asp718I AspEI AviII
BamHI BcgI BglII BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoT22I FspI HindIII HpaI
KpnI KspI MamI MluI MroI
NotI NruI NsiI PacI PaeR7I
PflMI PmeI PvuI RcaI RsrII
SacII SalI SexAI SfiI SgrAI
SnaBI SpeI SspBI SwaI Van91I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 249 GACGTC GACGT^C
AccI 2012 GTAGAC GT^[AC][GT]AC
AclI 2428 AACGTT AA^CGTT
AcsI 2046 GAATTT [GA]^AATT[CT]
AgeI 1609 ACCGGT A^CCGGT
Alw44I 3881 GTGCAC G^TGCAC
ApaLI 3881 GTGCAC G^TGCAC
ApoI 2046 GAATTT [GA]^AATT[CT]
AseI 3978 ATTAAT AT^TAAT
AsnI 3978 ATTAAT AT^TAAT
AspI 3913 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1726 CCCGGG C^[CT]CG[GA]G
AvrII 2462 CCTAGG C^CTAGG
BbvCI 976 CCTCAGC CCTCAGC(-5/-2)
BciVI 232 GGATAC GTATCC(6/5)
BclI 1372 TGATCA T^GATCA
BlnI 2462 CCTAGG C^CTAGG
BsaWI 1609 ACCGGT [AT]^CCGG[AT]
BsmBI 3145 GAGACG CGTCTC(1/5)
BsoBI 1726 CCCGGG C^[CT]CG[GA]G
BsrBI 808 GAGCGG CCGCTC(-3/-3)
BssHII 1445 GCGCGC G^CGCGC
BstAPI 1382 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3011 TTCGAA TT^CGAA
DraIII 1331 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2373 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 97 GATATC GAT^ATC
FbaI 1372 TGATCA T^GATCA
FseI 1755 GGCCGGCC GGCCGG^CC
HincII 3747 GTTGAC GT[CT]^[GA]AC
HindII 3747 GTTGAC GT[CT]^[GA]AC
MfeI 288 CAATTG C^AATTG
MunI 288 CAATTG C^AATTG
NaeI 1756 GCCGGC GCC^GGC
NdeI 1050 CATATG CA^TATG
NgoMIV 1756 GCCGGC G^CCGGC
NheI 2647 GCTAGC G^CTAGC
NspV 3011 TTCGAA TT^CGAA
PflFI 3913 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1609 ACCGGT A^CCGGT
PshAI 3147 GACGCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3978 ATTAAT AT^TAAT
Psp1406I 2428 AACGTT AA^CGTT
SapI 1966 GCTCTTC GCTCTTC(1/4)
SbfI 1785 CCTGCAGG CCTGCA^GG
ScaI 3857 AGTACT AGT^ACT
SfuI 3011 TTCGAA TT^CGAA
SmaI 1726 CCCGGG CCC^GGG
Sse8387I 1785 CCTGCAGG CCTGCA^GG
SspI 1655 AATATT AAT^ATT
StuI 3377 AGGCCT AGG^CCT
Tth111I 3913 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1726 CCCGGG C^CCGGG
XmaI 1726 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Aor51HI 497 AGCGCT AGC^GCT
727 AGCGCT
ApaI 297 GGGCCC GGGCC^C
1200 GGGCCC
BanI 173 GGCGCC G^G[CT][GA]CC
1815 GGCGCC
BbeI 173 GGCGCC GGCGC^C
1815 GGCGCC
EagI 71 CGGCCG C^GGCCG
1754 CGGCCG
EclXI 71 CGGCCG C^GGCCG
1754 CGGCCG
Eco47III 497 AGCGCT AGC^GCT
727 AGCGCT
Eco52I 71 CGGCCG C^GGCCG
1754 CGGCCG
KasI 173 GGCGCC G^GCGCC
1815 GGCGCC
NarI 173 GGCGCC GG^CGCC
1815 GGCGCC
SacI 276 GAGCTC GAGCT^C
1098 GAGCTC
SfoI 173 GGCGCC GGC^GCC
1815 GGCGCC
XbaI 2449 TCTAGA T^CTAGA
2942 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AflII
AluI AlwI AlwNI AspHI AvaII
BalI BanII BbrPI BbsI BbvI
BcnI BfaI BfrI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII