##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AgeI AhdI AscI
AspEI AspI AviII AvrII BbrPI
BciVI BclI BglII BlnI BsiEI
BsiWI BsmI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstZ17I
Cfr10I CpoI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI MfeI MluI
MunI NaeI NgoMIV NotI NruI
NsiI NspV PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacI SalI SexAI SfiI
SfuI SgrAI SnaBI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 1565 GGTACC G^GTACC
AccIII 48 TCCGGA T^CCGGA
AcyI 544 GGCGCC G[GA]^CG[CT]C
AflII 836 CTTAAG C^TTAAG
Alw44I 2374 GTGCAC G^TGCAC
ApaLI 2374 GTGCAC G^TGCAC
AseI 2621 ATTAAT AT^TAAT
AsnI 2621 ATTAAT AT^TAAT
Asp718I 1565 GGTACC G^GTACC
BbeI 544 GGCGCC GGCGC^C
BcgI 1059 GCACGTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 836 CTTAAG C^TTAAG
BlpI 2184 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2184 GCTCAGC GC^T[ACGT]AGC
BsaAI 3043 TACGTG [CT]AC^GT[GA]
BsaHI 544 GGCGCC G[GA]^CG[CT]C
BsaWI 48 TCCGGA [AT]^CCGG[AT]
BseAI 48 TCCGGA T^CCGGA
BsmBI 1016 GAGACG CGTCTC(1/5)
Bsp1407I 2316 TGTACA T^GTACA
BspDI 1067 ATCGAT AT^CGAT
BspEI 48 TCCGGA T^CCGGA
BsrDI 1888 CATTGC GCAATG(2/0)
BsrGI 2316 TGTACA T^GTACA
BstEII 206 GGTGACC G^GT[ACGT]ACC
BstPI 206 GGTGACC G^GT[ACGT]ACC
CelII 2184 GCTCAGC GC^T[ACGT]AGC
ClaI 1067 ATCGAT AT^CGAT
EcoO65I 206 GGTGACC G^GT[ACGT]ACC
EcoRI 1438 GAATTC G^AATTC
Hin1I 544 GGCGCC G[GA]^CG[CT]C
HincII 16 GTTGAC GT[CT]^[GA]AC
HindII 16 GTTGAC GT[CT]^[GA]AC
KasI 544 GGCGCC G^GCGCC
KpnI 1565 GGTACC GGTAC^C
KspI 202 CCGCGG CCGC^GG
MroI 48 TCCGGA T^CCGGA
NarI 544 GGCGCC GG^CGCC
NcoI 2485 CCATGG C^CATGG
NdeI 3409 CATATG CA^TATG
PacI 2696 TTAATTAA TTAAT^TAA
PaeR7I 288 CTCGAG C^TCGAG
PshBI 2621 ATTAAT AT^TAAT
PvuII 2741 CAGCTG CAG^CTG
SacII 202 CCGCGG CCGC^GG
SbfI 523 CCTGCAGG CCTGCA^GG
ScaI 798 AGTACT AGT^ACT
SfoI 544 GGCGCC GGC^GCC
SmaI 354 CCCGGG CCC^GGG
SpeI 1355 ACTAGT A^CTAGT
SphI 2098 GCATGC GCATG^C
Sse8387I 523 CCTGCAGG CCTGCA^GG
SspBI 2316 TGTACA T^GTACA
SspI 2618 AATATT AAT^ATT
XcmI 2802 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 288 CTCGAG C^TCGAG
XmaCI 354 CCCGGG C^CCGGG
XmaI 354 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 1667 CCTGAGG CC^T[ACGT]AGG
2295 CCTGAGG
Aor51HI 807 AGCGCT AGC^GCT
850 AGCGCT
Asp700I 304 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1132 GAAGCCGTTC
BamHI 216 GGATCC G^GATCC
456 GGATCC
BbvCI 2929 CCTCAGC CCTCAGC(-5/-2)
3697 GCTGAGG
BglI 723 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
979 GCCTTTCGGGC
BsaBI 457 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
899 GATGAAAATC
BspLU11I 1364 ACATGT A^CATGT
2311 ACATGT
BstXI 3160 CCATCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3736 CCAACTTCCTGG
Bsu36I 1667 CCTGAGG CC^T[ACGT]AGG
2295 CCTGAGG
DraI 2560 TTTAAA TTT^AAA
2580 TTTAAA
DrdI 1293 GACCTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3009 GACTCGCTTGTC
Eco47III 807 AGCGCT AGC^GCT
850 AGCGCT
Eco81I 1667 CCTGAGG CC^T[ACGT]AGG
2295 CCTGAGG
HgaI 1018 GACGC GACGC(5/10)
1655 GCGTC
MamI 457 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
899 GATGAAAATC
NheI 847 GCTAGC G^CTAGC
2066 GCTAGC
PshAI 1928 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3233 GACAGTGGTC
XmnI 304 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1132 GAAGCCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII