################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## Acc65I AccII AccIII AclI AgeI AocI AscI Asp718I AviII AvrII BbrPI BglII BlnI BsaAI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BssHII BstEII BstPI BstUI Bsu36I ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV FseI FspI HindIII KpnI KspI MluI MroI MvnI NdeI NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SnaBI SphI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AflII 3689 CTTAAG C^TTAAG AhdI 1703 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 314 AGCGCT AGC^GCT AseI 3004 ATTAAT AT^TAAT AsnI 3004 ATTAAT AT^TAAT AspEI 1703 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4090 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3495 CCCGGG C^[CT]CG[GA]G BbeI 28 GGCGCC GGCGC^C BbvCI 253 GCTGAGG CCTCAGC(-5/-2) BciVI 3348 GTATCC GTATCC(6/5) BfrI 3689 CTTAAG C^TTAAG BglI 3164 GCCAGTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2646 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2646 GCTGAGC GC^T[ACGT]AGC BsaWI 3281 TCCGGT [AT]^CCGG[AT] BsmBI 4088 GAGACG CGTCTC(1/5) BsoBI 3495 CCCGGG C^[CT]CG[GA]G Bsp1407I 3156 TGTACA T^GTACA BspHI 463 TCATGA T^CATGA BsrGI 3156 TGTACA T^GTACA BssSI 160 CACGAG CACGAG(-5/-1) Bst1107I 2585 GTATAC GTA^TAC BstBI 3121 TTCGAA TT^CGAA BstXI 2058 CCAAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2585 GTATAC GTA^TAC CelII 2646 GCTGAGC GC^T[ACGT]AGC DraI 4171 TTTAAA TTT^AAA Eam1105I 1703 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 314 AGCGCT AGC^GCT HincII 2623 GTTAAC GT[CT]^[GA]AC HindII 2623 GTTAAC GT[CT]^[GA]AC HpaI 2623 GTTAAC GTT^AAC KasI 28 GGCGCC G^GCGCC MfeI 2415 CAATTG C^AATTG MunI 2415 CAATTG C^AATTG NarI 28 GGCGCC GG^CGCC NcoI 3458 CCATGG C^CATGG NspV 3121 TTCGAA TT^CGAA PflFI 4090 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1964 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 3004 ATTAAT AT^TAAT RcaI 463 TCATGA T^CATGA SfoI 28 GGCGCC GGC^GCC SfuI 3121 TTCGAA TT^CGAA SgrAI 1505 CGCCGGCG C[GA]^CCGG[CT]G SmaI 3495 CCCGGG CCC^GGG SpeI 2879 ACTAGT A^CTAGT SspBI 3156 TGTACA T^GTACA StuI 800 AGGCCT AGG^CCT Tth111I 4090 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1964 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 3495 CCCGGG C^CCGGG XmaI 3495 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AatII 2276 GACGTC GACGT^C 3752 GACGTC AflIII 609 ACGTGT A^C[GA][CT]GT 2744 ACACGT Alw44I 983 GTGCAC G^TGCAC 2576 GTGCAC ApaI 216 GGGCCC GGGCC^C 1959 GGGCCC ApaLI 983 GTGCAC G^TGCAC 2576 GTGCAC Asp700I 312 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 588 GAAGCCATTC BalI 975 TGGCCA TGG^CCA 3959 TGGCCA BamHI 507 GGATCC G^GATCC 1996 GGATCC BclI 330 TGATCA T^GATCA 2184 TGATCA BsaBI 1030 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2182 GATGATCATC BsrFI 1506 GCCGGC [GA]^CCGG[CT] 2336 GCCGGC BstYI 507 GGATCC [GA]^GATC[CT] 1996 GGATCC Cfr10I 1506 GCCGGC [GA]^CCGG[CT] 2336 GCCGGC DraIII 1965 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG 4051 CACACAGTG EcoRI 62 GAATTC G^AATTC 3548 GAATTC EcoT22I 2600 ATGCAT ATGCA^T 2811 ATGCAT FbaI 330 TGATCA T^GATCA 2184 TGATCA MamI 1030 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2182 GATGATCATC MflI 507 GGATCC [GA]^GATC[CT] 1996 GGATCC MluNI 975 TGGCCA TGG^CCA 3959 TGGCCA MscI 975 TGGCCA TGG^CCA 3959 TGGCCA NaeI 1506 GCCGGC GCC^GGC 2336 GCCGGC NgoMIV 1506 GCCGGC G^CCGGC 2336 GCCGGC NsiI 2600 ATGCAT ATGCA^T 2811 ATGCAT PshAI 2272 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2876 GACACTAGTC SapI 298 GAAGAGC GCTCTTC(1/4) 2690 GAAGAGC XbaI 2732 TCTAGA T^CTAGA 2818 TCTAGA XcmI 87 CCACCTCCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2439 CCAGGGGATTCTTGG XhoII 507 GGATCC [GA]^GATC[CT] 1996 GGATCC XmnI 312 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 588 GAAGCCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I