##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AgeI        
AocI        AscI        Asp718I     AviII       AvrII       
BbrPI       BglII       BlnI        BsaAI       BseAI       
BsiEI       BsiWI       BspDI       BspEI       BspLU11I    
BssHII      BstEII      BstPI       BstUI       Bsu36I      
ClaI        CpoI        DrdI        EagI        EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
FseI        FspI        HindIII     KpnI        KspI        
MluI        MroI        MvnI        NdeI        NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SnaBI       SphI        
Sse8387I    SspI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AflII        3689  CTTAAG               C^TTAAG
AhdI         1703  GACCAACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       314  AGCGCT               AGC^GCT
AseI         3004  ATTAAT               AT^TAAT
AsnI         3004  ATTAAT               AT^TAAT
AspEI        1703  GACCAACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         4090  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         3495  CCCGGG               C^[CT]CG[GA]G
BbeI           28  GGCGCC               GGCGC^C
BbvCI         253  GCTGAGG              CCTCAGC(-5/-2)
BciVI        3348  GTATCC               GTATCC(6/5)
BfrI         3689  CTTAAG               C^TTAAG
BglI         3164  GCCAGTATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2646  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2646  GCTGAGC              GC^T[ACGT]AGC
BsaWI        3281  TCCGGT               [AT]^CCGG[AT]
BsmBI        4088  GAGACG               CGTCTC(1/5)
BsoBI        3495  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     3156  TGTACA               T^GTACA
BspHI         463  TCATGA               T^CATGA
BsrGI        3156  TGTACA               T^GTACA
BssSI         160  CACGAG               CACGAG(-5/-1)
Bst1107I     2585  GTATAC               GTA^TAC
BstBI        3121  TTCGAA               TT^CGAA
BstXI        2058  CCAAAACCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2585  GTATAC               GTA^TAC
CelII        2646  GCTGAGC              GC^T[ACGT]AGC
DraI         4171  TTTAAA               TTT^AAA
Eam1105I     1703  GACCAACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      314  AGCGCT               AGC^GCT
HincII       2623  GTTAAC               GT[CT]^[GA]AC
HindII       2623  GTTAAC               GT[CT]^[GA]AC
HpaI         2623  GTTAAC               GTT^AAC
KasI           28  GGCGCC               G^GCGCC
MfeI         2415  CAATTG               C^AATTG
MunI         2415  CAATTG               C^AATTG
NarI           28  GGCGCC               GG^CGCC
NcoI         3458  CCATGG               C^CATGG
NspV         3121  TTCGAA               TT^CGAA
PflFI        4090  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1964  CCACACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        3004  ATTAAT               AT^TAAT
RcaI          463  TCATGA               T^CATGA
SfoI           28  GGCGCC               GGC^GCC
SfuI         3121  TTCGAA               TT^CGAA
SgrAI        1505  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI         3495  CCCGGG               CCC^GGG
SpeI         2879  ACTAGT               A^CTAGT
SspBI        3156  TGTACA               T^GTACA
StuI          800  AGGCCT               AGG^CCT
Tth111I      4090  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1964  CCACACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        3495  CCCGGG               C^CCGGG
XmaI         3495  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AatII        2276  GACGTC               GACGT^C
             3752  GACGTC              
AflIII        609  ACGTGT               A^C[GA][CT]GT
             2744  ACACGT              
Alw44I        983  GTGCAC               G^TGCAC
             2576  GTGCAC              
ApaI          216  GGGCCC               GGGCC^C
             1959  GGGCCC              
ApaLI         983  GTGCAC               G^TGCAC
             2576  GTGCAC              
Asp700I       312  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              588  GAAGCCATTC          
BalI          975  TGGCCA               TGG^CCA
             3959  TGGCCA              
BamHI         507  GGATCC               G^GATCC
             1996  GGATCC              
BclI          330  TGATCA               T^GATCA
             2184  TGATCA              
BsaBI        1030  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2182  GATGATCATC          
BsrFI        1506  GCCGGC               [GA]^CCGG[CT]
             2336  GCCGGC              
BstYI         507  GGATCC               [GA]^GATC[CT]
             1996  GGATCC              
Cfr10I       1506  GCCGGC               [GA]^CCGG[CT]
             2336  GCCGGC              
DraIII       1965  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4051  CACACAGTG           
EcoRI          62  GAATTC               G^AATTC
             3548  GAATTC              
EcoT22I      2600  ATGCAT               ATGCA^T
             2811  ATGCAT              
FbaI          330  TGATCA               T^GATCA
             2184  TGATCA              
MamI         1030  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2182  GATGATCATC          
MflI          507  GGATCC               [GA]^GATC[CT]
             1996  GGATCC              
MluNI         975  TGGCCA               TGG^CCA
             3959  TGGCCA              
MscI          975  TGGCCA               TGG^CCA
             3959  TGGCCA              
NaeI         1506  GCCGGC               GCC^GGC
             2336  GCCGGC              
NgoMIV       1506  GCCGGC               G^CCGGC
             2336  GCCGGC              
NsiI         2600  ATGCAT               ATGCA^T
             2811  ATGCAT              
PshAI        2272  GACAGACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2876  GACACTAGTC          
SapI          298  GAAGAGC              GCTCTTC(1/4)
             2690  GAAGAGC             
XbaI         2732  TCTAGA               T^CTAGA
             2818  TCTAGA              
XcmI           87  CCACCTCCCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2439  CCAGGGGATTCTTGG     
XhoII         507  GGATCC               [GA]^GATC[CT]
             1996  GGATCC              
XmnI          312  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              588  GAAGCCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BanI        BanII       BbsI        BbvI        
BcgI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrBI       
BsrDI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I