##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AocI AscI Asp718I AviII AvrII
BbrPI BglII BlnI BsaAI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BssHII BstEII BstPI BstUI Bsu36I
ClaI CpoI DrdI EagI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRV
FseI FspI HindIII KpnI KspI
MluI MroI MvnI NdeI NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SnaBI SphI
Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AflII 3689 CTTAAG C^TTAAG
AhdI 1703 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 314 AGCGCT AGC^GCT
AseI 3004 ATTAAT AT^TAAT
AsnI 3004 ATTAAT AT^TAAT
AspEI 1703 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4090 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3495 CCCGGG C^[CT]CG[GA]G
BbeI 28 GGCGCC GGCGC^C
BbvCI 253 GCTGAGG CCTCAGC(-5/-2)
BciVI 3348 GTATCC GTATCC(6/5)
BfrI 3689 CTTAAG C^TTAAG
BglI 3164 GCCAGTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2646 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2646 GCTGAGC GC^T[ACGT]AGC
BsaWI 3281 TCCGGT [AT]^CCGG[AT]
BsmBI 4088 GAGACG CGTCTC(1/5)
BsoBI 3495 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3156 TGTACA T^GTACA
BspHI 463 TCATGA T^CATGA
BsrGI 3156 TGTACA T^GTACA
BssSI 160 CACGAG CACGAG(-5/-1)
Bst1107I 2585 GTATAC GTA^TAC
BstBI 3121 TTCGAA TT^CGAA
BstXI 2058 CCAAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2585 GTATAC GTA^TAC
CelII 2646 GCTGAGC GC^T[ACGT]AGC
DraI 4171 TTTAAA TTT^AAA
Eam1105I 1703 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 314 AGCGCT AGC^GCT
HincII 2623 GTTAAC GT[CT]^[GA]AC
HindII 2623 GTTAAC GT[CT]^[GA]AC
HpaI 2623 GTTAAC GTT^AAC
KasI 28 GGCGCC G^GCGCC
MfeI 2415 CAATTG C^AATTG
MunI 2415 CAATTG C^AATTG
NarI 28 GGCGCC GG^CGCC
NcoI 3458 CCATGG C^CATGG
NspV 3121 TTCGAA TT^CGAA
PflFI 4090 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1964 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3004 ATTAAT AT^TAAT
RcaI 463 TCATGA T^CATGA
SfoI 28 GGCGCC GGC^GCC
SfuI 3121 TTCGAA TT^CGAA
SgrAI 1505 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3495 CCCGGG CCC^GGG
SpeI 2879 ACTAGT A^CTAGT
SspBI 3156 TGTACA T^GTACA
StuI 800 AGGCCT AGG^CCT
Tth111I 4090 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1964 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3495 CCCGGG C^CCGGG
XmaI 3495 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 2276 GACGTC GACGT^C
3752 GACGTC
AflIII 609 ACGTGT A^C[GA][CT]GT
2744 ACACGT
Alw44I 983 GTGCAC G^TGCAC
2576 GTGCAC
ApaI 216 GGGCCC GGGCC^C
1959 GGGCCC
ApaLI 983 GTGCAC G^TGCAC
2576 GTGCAC
Asp700I 312 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
588 GAAGCCATTC
BalI 975 TGGCCA TGG^CCA
3959 TGGCCA
BamHI 507 GGATCC G^GATCC
1996 GGATCC
BclI 330 TGATCA T^GATCA
2184 TGATCA
BsaBI 1030 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2182 GATGATCATC
BsrFI 1506 GCCGGC [GA]^CCGG[CT]
2336 GCCGGC
BstYI 507 GGATCC [GA]^GATC[CT]
1996 GGATCC
Cfr10I 1506 GCCGGC [GA]^CCGG[CT]
2336 GCCGGC
DraIII 1965 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
4051 CACACAGTG
EcoRI 62 GAATTC G^AATTC
3548 GAATTC
EcoT22I 2600 ATGCAT ATGCA^T
2811 ATGCAT
FbaI 330 TGATCA T^GATCA
2184 TGATCA
MamI 1030 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2182 GATGATCATC
MflI 507 GGATCC [GA]^GATC[CT]
1996 GGATCC
MluNI 975 TGGCCA TGG^CCA
3959 TGGCCA
MscI 975 TGGCCA TGG^CCA
3959 TGGCCA
NaeI 1506 GCCGGC GCC^GGC
2336 GCCGGC
NgoMIV 1506 GCCGGC G^CCGGC
2336 GCCGGC
NsiI 2600 ATGCAT ATGCA^T
2811 ATGCAT
PshAI 2272 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2876 GACACTAGTC
SapI 298 GAAGAGC GCTCTTC(1/4)
2690 GAAGAGC
XbaI 2732 TCTAGA T^CTAGA
2818 TCTAGA
XcmI 87 CCACCTCCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2439 CCAGGGGATTCTTGG
XhoII 507 GGATCC [GA]^GATC[CT]
1996 GGATCC
XmnI 312 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
588 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I