##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
Aor51HI AscI AseI AsnI BcgI
BciVI BclI BfrI BsaBI BsiWI
BsmI BspDI BspHI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 942 TCCGGA T^CCGGA
Alw44I 797 GTGCAC G^TGCAC
ApaI 2156 GGGCCC GGGCC^C
ApaLI 797 GTGCAC G^TGCAC
Asp700I 3998 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2196 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2948 TGCGCA TGC^GCA
BbeI 2422 GGCGCC GGCGC^C
BbrPI 1984 CACGTG CAC^GTG
BglII 711 AGATCT A^GATCT
BseAI 942 TCCGGA T^CCGGA
BsiEI 182 CGGCCG CG[GA][CT]^CG
Bsp1407I 4056 TGTACA T^GTACA
BspEI 942 TCCGGA T^CCGGA
BsrDI 4172 GCAATG GCAATG(2/0)
BsrGI 4056 TGTACA T^GTACA
DraI 4101 TTTAAA TTT^AAA
DrdI 2529 GACTCTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 182 CGGCCG C^GGCCG
EarI 707 GAAGAG CTCTTC(1/4)
EclXI 182 CGGCCG C^GGCCG
Eco52I 182 CGGCCG C^GGCCG
FspI 2948 TGCGCA TGC^GCA
KasI 2422 GGCGCC G^GCGCC
Ksp632I 707 GAAGAG CTCTTC(1/4)
MroI 942 TCCGGA T^CCGGA
MslI 957 CACGTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2422 GGCGCC GG^CGCC
NspI 1046 ACATGC [GA]CATG^[CT]
PaeR7I 3746 CTCGAG C^TCGAG
PflFI 2196 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1984 CACGTG CAC^GTG
PmlI 1984 CACGTG CAC^GTG
SacI 669 GAGCTC GAGCT^C
SbfI 2146 CCTGCAGG CCTGCA^GG
ScaI 1612 AGTACT AGT^ACT
SfoI 2422 GGCGCC GGC^GCC
Sse8387I 2146 CCTGCAGG CCTGCA^GG
SspBI 4056 TGTACA T^GTACA
SspI 3999 AATATT AAT^ATT
Tth111I 2196 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3746 CTCGAG C^TCGAG
XmnI 3998 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1168 GTCTAC GT^[AC][GT]AC
2575 GTCTAC
AccII 806 CGCG CG^CG
3341 CGCG
AcsI 3877 AAATTC [GA]^AATT[CT]
4136 GAATTT
AcyI 2422 GGCGCC G[GA]^CG[CT]C
2535 GGCGTC
AhdI 1321 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2431 GACTCCGGGTC
ApoI 3877 AAATTC [GA]^AATT[CT]
4136 GAATTT
AspEI 1321 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2431 GACTCCGGGTC
AvrII 1912 CCTAGG C^CTAGG
3474 CCTAGG
BamHI 2319 GGATCC G^GATCC
4311 GGATCC
BlnI 1912 CCTAGG C^CTAGG
3474 CCTAGG
BlpI 991 GCTGAGC GC^T[ACGT]AGC
1360 GCTCAGC
Bpu1102I 991 GCTGAGC GC^T[ACGT]AGC
1360 GCTCAGC
BsaAI 957 CACGTA [CT]AC^GT[GA]
1984 CACGTG
BsaHI 2422 GGCGCC G[GA]^CG[CT]C
2535 GGCGTC
BsaWI 942 TCCGGA [AT]^CCGG[AT]
3313 TCCGGT
BsmBI 3466 CGTCTC CGTCTC(1/5)
3722 GAGACG
BsrBI 3043 CCGCTC CCGCTC(-3/-3)
3961 GAGCGG
BssSI 794 CTCGTG CACGAG(-5/-1)
3410 CTCGTG
BstEII 909 GGTGACC G^GT[ACGT]ACC
4037 GGTGACC
BstPI 909 GGTGACC G^GT[ACGT]ACC
4037 GGTGACC
BstUI 806 CGCG CG^CG
3341 CGCG
CelII 991 GCTGAGC GC^T[ACGT]AGC
1360 GCTCAGC
DraIII 505 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
2270 CACCCAGTG
Eam1105I 1321 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2431 GACTCCGGGTC
EcoNI 1371 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3526 CCTGAGCCAGG
EcoO65I 909 GGTGACC G^GT[ACGT]ACC
4037 GGTGACC
Hin1I 2422 GGCGCC G[GA]^CG[CT]C
2535 GGCGTC
MseI 139 TTAA T^TAA
4102 TTAA
MvnI 806 CGCG CG^CG
3341 CGCG
NaeI 180 GCCGGC GCC^GGC
824 GCCGGC
NgoMIV 180 GCCGGC G^CCGGC
824 GCCGGC
PstI 2147 CTGCAG CTGCA^G
4150 CTGCAG
SmaI 2426 CCCGGG CCC^GGG
3243 CCCGGG
Tru9I 139 TTAA T^TAA
4102 TTAA
XmaCI 2426 CCCGGG C^CCGGG
3243 CCCGGG
XmaI 2426 CCCGGG C^CCGGG
3243 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
AlwNI AocI Asp718I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII