##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BcgI BclI BcnI
BglII BlnI BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI BstYI Cfr10I ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NgoMIV NotI NruI
NspV PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
XbaI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2904 GTATAC GT^[AC][GT]AC
AlwI 41 GATCC GGATC(4/5)
AocI 1827 CCTGAGG CC^T[ACGT]AGG
AspI 835 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2127 CCTCAGC CCTCAGC(-5/-2)
BciVI 129 GTATCC GTATCC(6/5)
BglI 2356 GCCACATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2212 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2212 GCTGAGC GC^T[ACGT]AGC
BsaWI 1845 TCCGGT [AT]^CCGG[AT]
Bsp1407I 2912 TGTACA T^GTACA
BsrGI 2912 TGTACA T^GTACA
Bst1107I 2904 GTATAC GTA^TAC
BstXI 381 CCAGACAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2904 GTATAC GTA^TAC
Bsu36I 1827 CCTGAGG CC^T[ACGT]AGG
CelII 2212 GCTGAGC GC^T[ACGT]AGC
Eco81I 1827 CCTGAGG CC^T[ACGT]AGG
FauI 3150 GCGGG CCCGC(4/6)
HaeII 2756 AGCGCC [GA]GCGC^[CT]
HgaI 839 GCGTC GACGC(5/10)
NdeI 1981 CATATG CA^TATG
PacI 3716 TTAATTAA TTAAT^TAA
PaeR7I 3239 CTCGAG C^TCGAG
PflFI 835 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 473 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 2177 GAAGAGC GCTCTTC(1/4)
ScaI 3187 AGTACT AGT^ACT
SspBI 2912 TGTACA T^GTACA
Tth111I 835 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 473 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3239 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 3150 GCGG CCGC(-3/-1)
3478 GCGG
AflII 920 CTTAAG C^TTAAG
1259 CTTAAG
AvaI 3134 CTCGGG C^[CT]CG[GA]G
3239 CTCGAG
BanII 1121 GAGCCC G[GA]GC[CT]^C
2805 GGGCTC
BbsI 3664 GAAGAC GAAGAC(2/6)
2534 GTCTTC
BfrI 920 CTTAAG C^TTAAG
1259 CTTAAG
BpuAI 3664 GAAGAC GAAGAC(2/6)
2534 GTCTTC
BsaI 1140 GAGACC GGTCTC(1/5)
1070 GGTCTC
BsmI 928 GAATGC GAATGC(1/-1)
2308 GCATTC
BsoBI 3134 CTCGGG C^[CT]CG[GA]G
3239 CTCGAG
BspMI 224 ACCTGC ACCTGC(4/8)
3332 ACCTGC
DrdI 907 GACTTCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2394 GACTTTTTTGTC
EarI 2177 GAAGAG CTCTTC(1/4)
3250 GAAGAG
HapII 1846 CCGG C^CGG
3076 CCGG
HincII 1233 GTTGAC GT[CT]^[GA]AC
2167 GTCAAC
HindII 1233 GTTGAC GT[CT]^[GA]AC
2167 GTCAAC
HpaII 1846 CCGG C^CGG
3076 CCGG
HphI 720 GGTGA GGTGA(8/7)
3490 GGTGA
Ksp632I 2177 GAAGAG CTCTTC(1/4)
3250 GAAGAG
MaeII 2794 ACGT A^CGT
3395 ACGT
MspI 1846 CCGG C^CGG
3076 CCGG
NheI 2521 GCTAGC G^CTAGC
2582 GCTAGC
PpuMI 425 AGGTCCT [GA]G^G[AT]CC[CT]
2745 GGGTCCT
SphI 604 GCATGC GCATG^C
2920 GCATGC
SspI 191 AATATT AAT^ATT
889 AATATT
XcmI 160 CCAGGAGGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3389 CCACTTACGTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AcsI AfaI AluI Alw44I AlwNI
ApaLI ApoI AseI AsnI AspHI
AvaII BbvI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I