################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BclI BfrI BglII BlnI BsiWI BsmI BspLU11I BssHII Bst1107I BstBI BstZ17I CpoI DraI EagI EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI HpaI KpnI KspI MfeI MluI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I RsrII SacII SalI SbfI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tru9I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AccIII 1574 TCCGGA T^CCGGA AspI 1111 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 892 CACGTG CAC^GTG BciVI 1392 GTATCC GTATCC(6/5) BsaBI 2049 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1574 TCCGGA T^CCGGA BspDI 1621 ATCGAT AT^CGAT BspEI 1574 TCCGGA T^CCGGA ClaI 1621 ATCGAT AT^CGAT EcoRI 1705 GAATTC G^AATTC HincII 2566 GTTGAC GT[CT]^[GA]AC HindII 2566 GTTGAC GT[CT]^[GA]AC HindIII 2590 AAGCTT A^AGCTT MamI 2049 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1574 TCCGGA T^CCGGA NaeI 880 GCCGGC GCC^GGC NgoMIV 880 GCCGGC G^CCGGC PflFI 1111 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 892 CACGTG CAC^GTG PmlI 892 CACGTG CAC^GTG PvuI 1619 CGATCG CGAT^CG SacI 326 GAGCTC GAGCT^C SapI 1278 GCTCTTC GCTCTTC(1/4) ScaI 866 AGTACT AGT^ACT StuI 2843 AGGCCT AGG^CCT Tth111I 1111 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3291 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 46 enzymes) # ################################### AccI 849 GTCTAC GT^[AC][GT]AC 1297 GTCTAC AccII 409 CGCG CG^CG 943 CGCG AhdI 378 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3184 GACGTGGGGTC Aor51HI 1493 AGCGCT AGC^GCT 1726 AGCGCT AspEI 378 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3184 GACGTGGGGTC BalI 935 TGGCCA TGG^CCA 2315 TGGCCA BbeI 2327 GGCGCC GGCGC^C 3555 GGCGCC BbvCI 2109 CCTCAGC CCTCAGC(-5/-2) 2490 GCTGAGG BcgI 2266 CGAAAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1628 GCATCCGCTTCG BlpI 1729 GCTGAGC GC^T[ACGT]AGC 2630 GCTCAGC Bpu1102I 1729 GCTGAGC GC^T[ACGT]AGC 2630 GCTCAGC BsaAI 892 CACGTG [CT]AC^GT[GA] 3310 TACGTG BsaWI 1254 TCCGGT [AT]^CCGG[AT] 1574 TCCGGA BsmBI 1780 CGTCTC CGTCTC(1/5) 2913 CGTCTC Bsp1407I 144 TGTACA T^GTACA 3452 TGTACA BspHI 71 TCATGA T^CATGA 3534 TCATGA BsrGI 144 TGTACA T^GTACA 3452 TGTACA BssSI 1120 CACGAG CACGAG(-5/-1) 3435 CACGAG BstEII 301 GGTCACC G^GT[ACGT]ACC 2194 GGTGACC BstPI 301 GGTCACC G^GT[ACGT]ACC 2194 GGTGACC BstUI 409 CGCG CG^CG 943 CGCG BstXI 104 CCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1451 CCACGGTGCTGG BstYI 992 AGATCC [GA]^GATC[CT] 1064 AGATCC CelII 1729 GCTGAGC GC^T[ACGT]AGC 2630 GCTCAGC DraIII 808 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 3488 CACACTGTG DrdI 1186 GACCTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1414 GACCTTCTTGTC DsaI 1351 CCACGG C^C[GA][CT]GG 1451 CCACGG Eam1105I 378 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3184 GACGTGGGGTC Eco47III 1493 AGCGCT AGC^GCT 1726 AGCGCT EcoNI 1358 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2546 CCTCCCAGAGG EcoO65I 301 GGTCACC G^GT[ACGT]ACC 2194 GGTGACC KasI 2327 GGCGCC G^GCGCC 3555 GGCGCC MflI 992 AGATCC [GA]^GATC[CT] 1064 AGATCC MluNI 935 TGGCCA TGG^CCA 2315 TGGCCA MscI 935 TGGCCA TGG^CCA 2315 TGGCCA MslI 1197 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1449 CACCACGGTG MvnI 409 CGCG CG^CG 943 CGCG NarI 2327 GGCGCC GG^CGCC 3555 GGCGCC PshAI 38 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1608 GACCTCAGTC RcaI 71 TCATGA T^CATGA 3534 TCATGA SfiI 1744 GGCCGAGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3144 GGCCCGCCAGGCC SfoI 2327 GGCGCC GGC^GCC 3555 GGCGCC SphI 527 GCATGC GCATG^C 898 GCATGC SspBI 144 TGTACA T^GTACA 3452 TGTACA XcmI 101 CCACCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2066 CCATGTTCAGCCTGG XhoII 992 AGATCC [GA]^GATC[CT] 1064 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I