##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BclI BfrI BglII BlnI
BsiWI BsmI BspLU11I BssHII Bst1107I
BstBI BstZ17I CpoI DraI EagI
EclXI Eco52I EcoRV EcoT22I FbaI
FseI FspI HpaI KpnI KspI
MfeI MluI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I RsrII SacII SalI
SbfI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 1574 TCCGGA T^CCGGA
AspI 1111 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 892 CACGTG CAC^GTG
BciVI 1392 GTATCC GTATCC(6/5)
BsaBI 2049 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1574 TCCGGA T^CCGGA
BspDI 1621 ATCGAT AT^CGAT
BspEI 1574 TCCGGA T^CCGGA
ClaI 1621 ATCGAT AT^CGAT
EcoRI 1705 GAATTC G^AATTC
HincII 2566 GTTGAC GT[CT]^[GA]AC
HindII 2566 GTTGAC GT[CT]^[GA]AC
HindIII 2590 AAGCTT A^AGCTT
MamI 2049 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1574 TCCGGA T^CCGGA
NaeI 880 GCCGGC GCC^GGC
NgoMIV 880 GCCGGC G^CCGGC
PflFI 1111 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 892 CACGTG CAC^GTG
PmlI 892 CACGTG CAC^GTG
PvuI 1619 CGATCG CGAT^CG
SacI 326 GAGCTC GAGCT^C
SapI 1278 GCTCTTC GCTCTTC(1/4)
ScaI 866 AGTACT AGT^ACT
StuI 2843 AGGCCT AGG^CCT
Tth111I 1111 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3291 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 46 enzymes) #
###################################
AccI 849 GTCTAC GT^[AC][GT]AC
1297 GTCTAC
AccII 409 CGCG CG^CG
943 CGCG
AhdI 378 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3184 GACGTGGGGTC
Aor51HI 1493 AGCGCT AGC^GCT
1726 AGCGCT
AspEI 378 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3184 GACGTGGGGTC
BalI 935 TGGCCA TGG^CCA
2315 TGGCCA
BbeI 2327 GGCGCC GGCGC^C
3555 GGCGCC
BbvCI 2109 CCTCAGC CCTCAGC(-5/-2)
2490 GCTGAGG
BcgI 2266 CGAAAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1628 GCATCCGCTTCG
BlpI 1729 GCTGAGC GC^T[ACGT]AGC
2630 GCTCAGC
Bpu1102I 1729 GCTGAGC GC^T[ACGT]AGC
2630 GCTCAGC
BsaAI 892 CACGTG [CT]AC^GT[GA]
3310 TACGTG
BsaWI 1254 TCCGGT [AT]^CCGG[AT]
1574 TCCGGA
BsmBI 1780 CGTCTC CGTCTC(1/5)
2913 CGTCTC
Bsp1407I 144 TGTACA T^GTACA
3452 TGTACA
BspHI 71 TCATGA T^CATGA
3534 TCATGA
BsrGI 144 TGTACA T^GTACA
3452 TGTACA
BssSI 1120 CACGAG CACGAG(-5/-1)
3435 CACGAG
BstEII 301 GGTCACC G^GT[ACGT]ACC
2194 GGTGACC
BstPI 301 GGTCACC G^GT[ACGT]ACC
2194 GGTGACC
BstUI 409 CGCG CG^CG
943 CGCG
BstXI 104 CCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1451 CCACGGTGCTGG
BstYI 992 AGATCC [GA]^GATC[CT]
1064 AGATCC
CelII 1729 GCTGAGC GC^T[ACGT]AGC
2630 GCTCAGC
DraIII 808 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3488 CACACTGTG
DrdI 1186 GACCTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1414 GACCTTCTTGTC
DsaI 1351 CCACGG C^C[GA][CT]GG
1451 CCACGG
Eam1105I 378 GACTCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3184 GACGTGGGGTC
Eco47III 1493 AGCGCT AGC^GCT
1726 AGCGCT
EcoNI 1358 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2546 CCTCCCAGAGG
EcoO65I 301 GGTCACC G^GT[ACGT]ACC
2194 GGTGACC
KasI 2327 GGCGCC G^GCGCC
3555 GGCGCC
MflI 992 AGATCC [GA]^GATC[CT]
1064 AGATCC
MluNI 935 TGGCCA TGG^CCA
2315 TGGCCA
MscI 935 TGGCCA TGG^CCA
2315 TGGCCA
MslI 1197 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1449 CACCACGGTG
MvnI 409 CGCG CG^CG
943 CGCG
NarI 2327 GGCGCC GG^CGCC
3555 GGCGCC
PshAI 38 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1608 GACCTCAGTC
RcaI 71 TCATGA T^CATGA
3534 TCATGA
SfiI 1744 GGCCGAGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3144 GGCCCGCCAGGCC
SfoI 2327 GGCGCC GGC^GCC
3555 GGCGCC
SphI 527 GCATGC GCATG^C
898 GCATGC
SspBI 144 TGTACA T^GTACA
3452 TGTACA
XcmI 101 CCACCAGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2066 CCATGTTCAGCCTGG
XhoII 992 AGATCC [GA]^GATC[CT]
1064 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I