##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I AspEI AspI
BamHI BbrPI BciVI BclI BfrI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FbaI FseI HindIII HpaI
KspI MamI MfeI MluI MroI
MunI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 2383 GGTACC G^GTACC
AccII 2906 CGCG CG^CG
Aor51HI 2760 AGCGCT AGC^GCT
Asp718I 2383 GGTACC G^GTACC
AviII 265 TGCGCA TGC^GCA
BcgI 3374 CGAGTGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 140 ACCGGA [AT]^CCGG[AT]
BsmI 591 GCATTC GAATGC(1/-1)
BspHI 3024 TCATGA T^CATGA
BsrBI 273 GAGCGG CCGCTC(-3/-3)
BssSI 3387 CACGAG CACGAG(-5/-1)
BstUI 2906 CGCG CG^CG
DraI 1428 TTTAAA TTT^AAA
DrdI 4006 GACAAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2760 AGCGCT AGC^GCT
FspI 265 TGCGCA TGC^GCA
KpnI 2383 GGTACC GGTAC^C
MvnI 2906 CGCG CG^CG
NaeI 3724 GCCGGC GCC^GGC
NgoMIV 3724 GCCGGC G^CCGGC
NheI 378 GCTAGC G^CTAGC
PflMI 845 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1844 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3024 TCATGA T^CATGA
SacI 159 GAGCTC GAGCT^C
SfiI 569 GGCCTGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2727 GCATGC GCATG^C
Van91I 845 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2852 GACGTC GACGT^C
3944 GACGTC
AcsI 2396 AAATTC [GA]^AATT[CT]
4450 AAATTT
AlwI 1647 GATCC GGATC(4/5)
2327 GGATC
ApaI 300 GGGCCC GGGCC^C
1063 GGGCCC
ApoI 2396 AAATTC [GA]^AATT[CT]
4450 AAATTT
AvaI 395 CCCGGG C^[CT]CG[GA]G
3181 CCCGGG
AvrII 1601 CCTAGG C^CTAGG
3459 CCTAGG
BbeI 2889 GGCGCC GGCGC^C
3398 GGCGCC
BbvCI 1881 CCTCAGC CCTCAGC(-5/-2)
2740 CCTCAGC
BglII 819 AGATCT A^GATCT
1365 AGATCT
BlnI 1601 CCTAGG C^CTAGG
3459 CCTAGG
BsaI 1561 GGTCTC GGTCTC(1/5)
3210 GGTCTC
BsoBI 395 CCCGGG C^[CT]CG[GA]G
3181 CCCGGG
Bsp1407I 980 TGTACA T^GTACA
4279 TGTACA
BsrFI 3319 GCCGGT [GA]^CCGG[CT]
3724 GCCGGC
BsrGI 980 TGTACA T^GTACA
4279 TGTACA
Cfr10I 3319 GCCGGT [GA]^CCGG[CT]
3724 GCCGGC
EcoNI 2430 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3459 CCTAGGCCAGG
EcoT22I 3432 ATGCAT ATGCA^T
3826 ATGCAT
FauI 2904 CCCGC CCCGC(4/6)
275 GCGGG
HgaI 203 GACGC GACGC(5/10)
2955 GCGTC
HincII 1245 GTTGAC GT[CT]^[GA]AC
2650 GTCAAC
HindII 1245 GTTGAC GT[CT]^[GA]AC
2650 GTCAAC
KasI 2889 GGCGCC G^GCGCC
3398 GGCGCC
NarI 2889 GGCGCC GG^CGCC
3398 GGCGCC
NcoI 2070 CCATGG C^CATGG
2335 CCATGG
NdeI 618 CATATG CA^TATG
984 CATATG
NsiI 3432 ATGCAT ATGCA^T
3826 ATGCAT
PvuII 2233 CAGCTG CAG^CTG
3842 CAGCTG
SexAI 1557 ACCAGGT A^CC[AT]GGT
3256 ACCTGGT
SfoI 2889 GGCGCC GGC^GCC
3398 GGCGCC
SmaI 395 CCCGGG CCC^GGG
3181 CCCGGG
SspBI 980 TGTACA T^GTACA
4279 TGTACA
SspI 1442 AATATT AAT^ATT
3510 AATATT
TfiI 658 GAATC G^A[AT]TC
1980 GATTC
XmaCI 395 CCCGGG C^CCGGG
3181 CCCGGG
XmaI 395 CCCGGG C^CCGGG
3181 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AocI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII