##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BciVI BfrI BlnI BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI BstBI ClaI CpoI DraI
Eam1105I EcoRV FspI HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 4017 ACACGT A^C[GA][CT]GT
Aor51HI 563 AGCGCT AGC^GCT
Asp700I 876 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 3793 TGGCCA TGG^CCA
BbeI 3570 GGCGCC GGCGC^C
BglII 656 AGATCT A^GATCT
BsiEI 1201 CGGCCG CG[GA][CT]^CG
BsmI 1671 GAATGC GAATGC(1/-1)
BsrDI 921 CATTGC GCAATG(2/0)
Bst1107I 4177 GTATAC GTA^TAC
BstZ17I 4177 GTATAC GTA^TAC
DraIII 1210 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1201 CGGCCG C^GGCCG
EclXI 1201 CGGCCG C^GGCCG
Eco47III 563 AGCGCT AGC^GCT
Eco52I 1201 CGGCCG C^GGCCG
EcoRI 2922 GAATTC G^AATTC
EcoT22I 3981 ATGCAT ATGCA^T
FseI 4095 GGCCGGCC GGCCGG^CC
KasI 3570 GGCGCC G^GCGCC
KspI 3575 CCGCGG CCGC^GG
MluNI 3793 TGGCCA TGG^CCA
MscI 3793 TGGCCA TGG^CCA
NaeI 4096 GCCGGC GCC^GGC
NarI 3570 GGCGCC GG^CGCC
NgoMIV 4096 GCCGGC G^CCGGC
NsiI 3981 ATGCAT ATGCA^T
NspI 2348 ACATGC [GA]CATG^[CT]
PaeR7I 1597 CTCGAG C^TCGAG
PshAI 2966 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3101 GAGCTC GAGCT^C
SacII 3575 CCGCGG CCGC^GG
SbfI 2340 CCTGCAGG CCTGCA^GG
SexAI 4082 ACCTGGT A^CC[AT]GGT
SfoI 3570 GGCGCC GGC^GCC
Sse8387I 2340 CCTGCAGG CCTGCA^GG
XbaI 2092 TCTAGA T^CTAGA
XhoI 1597 CTCGAG C^TCGAG
XmnI 876 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1063 GTCTAC GT^[AC][GT]AC
4177 GTATAC
AcsI 696 GAATTT [GA]^AATT[CT]
2922 GAATTC
ApoI 696 GAATTT [GA]^AATT[CT]
2922 GAATTC
BanI 1895 GGTGCC G^G[CT][GA]CC
3570 GGCGCC
BbrPI 3621 CACGTG CAC^GTG
4018 CACGTG
BbvCI 2567 CCTCAGC CCTCAGC(-5/-2)
166 GCTGAGG
BclI 2736 TGATCA T^GATCA
4022 TGATCA
BlpI 820 GCTCAGC GC^T[ACGT]AGC
1320 GCTGAGC
Bpu1102I 820 GCTCAGC GC^T[ACGT]AGC
1320 GCTGAGC
BsmBI 2772 CGTCTC CGTCTC(1/5)
2932 GAGACG
BsrFI 3974 ACCGGC [GA]^CCGG[CT]
4096 GCCGGC
BstAPI 2343 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3742 GCAGCCCGTGC
BstEII 3534 GGTCACC G^GT[ACGT]ACC
4336 GGTCACC
BstPI 3534 GGTCACC G^GT[ACGT]ACC
4336 GGTCACC
BstXI 2378 CCAAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2435 CCAGGATCATGG
CelII 820 GCTCAGC GC^T[ACGT]AGC
1320 GCTGAGC
Cfr10I 3974 ACCGGC [GA]^CCGG[CT]
4096 GCCGGC
DrdI 1029 GACAGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1485 GACCAGCGGGTC
EcoO65I 3534 GGTCACC G^GT[ACGT]ACC
4336 GGTCACC
FbaI 2736 TGATCA T^GATCA
4022 TGATCA
HaeII 563 AGCGCT [GA]GCGC^[CT]
3570 GGCGCC
HincII 3216 GTTGAC GT[CT]^[GA]AC
4013 GTCAAC
HindII 3216 GTTGAC GT[CT]^[GA]AC
4013 GTCAAC
PmaCI 3621 CACGTG CAC^GTG
4018 CACGTG
PmlI 3621 CACGTG CAC^GTG
4018 CACGTG
ScaI 174 AGTACT AGT^ACT
341 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BanII
BbsI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII