##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AclI AcsI AgeI ApoI AscI
AseI AsnI AvrII BcgI BglII
BlnI BsiWI BspLU11I BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I CpoI
DraI EcoRI EcoT22I FseI HincII
HindII HpaI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Van91I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 2350 CGCG CG^CG
AccIII 1568 TCCGGA T^CCGGA
AflII 2965 CTTAAG C^TTAAG
AflIII 2013 ACGTGT A^C[GA][CT]GT
AhdI 2914 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 750 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2735 AGCGCT AGC^GCT
Asp700I 2373 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2914 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2120 TGGCCA TGG^CCA
BamHI 1527 GGATCC G^GATCC
BbeI 523 GGCGCC GGCGC^C
BbrPI 2277 CACGTG CAC^GTG
BclI 2852 TGATCA T^GATCA
BfrI 2965 CTTAAG C^TTAAG
BglI 2653 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1875 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1568 TCCGGA T^CCGGA
BsmI 2373 GAATGC GAATGC(1/-1)
BspDI 3229 ATCGAT AT^CGAT
BspEI 1568 TCCGGA T^CCGGA
BsrBI 2106 CCGCTC CCGCTC(-3/-3)
BstUI 2350 CGCG CG^CG
Bsu36I 750 CCTGAGG CC^T[ACGT]AGG
ClaI 3229 ATCGAT AT^CGAT
DrdI 3458 GACGGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2914 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2735 AGCGCT AGC^GCT
Eco81I 750 CCTGAGG CC^T[ACGT]AGG
EcoRV 2320 GATATC GAT^ATC
FbaI 2852 TGATCA T^GATCA
KasI 523 GGCGCC G^GCGCC
KspI 2349 CCGCGG CCGC^GG
MamI 1875 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2120 TGGCCA TGG^CCA
MroI 1568 TCCGGA T^CCGGA
MscI 2120 TGGCCA TGG^CCA
MvnI 2350 CGCG CG^CG
NarI 523 GGCGCC GG^CGCC
NcoI 1475 CCATGG C^CATGG
PmaCI 2277 CACGTG CAC^GTG
PmlI 2277 CACGTG CAC^GTG
SacI 2947 GAGCTC GAGCT^C
SacII 2349 CCGCGG CCGC^GG
SexAI 1817 ACCAGGT A^CC[AT]GGT
SfoI 523 GGCGCC GGC^GCC
StuI 2651 AGGCCT AGG^CCT
XbaI 94 TCTAGA T^CTAGA
XmnI 2373 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 380 GGTACC G^GTACC
1208 GGTACC
AccI 637 GTCTAC GT^[AC][GT]AC
3235 GTCTAC
Alw44I 2280 GTGCAC G^TGCAC
2713 GTGCAC
ApaI 147 GGGCCC GGGCC^C
1545 GGGCCC
ApaLI 2280 GTGCAC G^TGCAC
2713 GTGCAC
Asp718I 380 GGTACC G^GTACC
1208 GGTACC
AspI 1816 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1936 GACGTCGTC
AviII 785 TGCGCA TGC^GCA
2222 TGCGCA
BfaI 41 CTAG C^TAG
95 CTAG
BlpI 2086 GCTAAGC GC^T[ACGT]AGC
3581 GCTAAGC
Bpu1102I 2086 GCTAAGC GC^T[ACGT]AGC
3581 GCTAAGC
BsaAI 2012 TACGTG [CT]AC^GT[GA]
2277 CACGTG
BsmBI 2215 GAGACG CGTCTC(1/5)
3327 GAGACG
BspHI 1073 TCATGA T^CATGA
3161 TCATGA
CelII 2086 GCTAAGC GC^T[ACGT]AGC
3581 GCTAAGC
EagI 1200 CGGCCG C^GGCCG
2697 CGGCCG
EclXI 1200 CGGCCG C^GGCCG
2697 CGGCCG
Eco52I 1200 CGGCCG C^GGCCG
2697 CGGCCG
FspI 785 TGCGCA TGC^GCA
2222 TGCGCA
HgaI 1767 GACGC GACGC(5/10)
2217 GACGC
HindIII 820 AAGCTT A^AGCTT
3649 AAGCTT
KpnI 380 GGTACC GGTAC^C
1208 GGTACC
MaeI 41 CTAG C^TAG
95 CTAG
PflFI 1816 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1936 GACGTCGTC
RcaI 1073 TCATGA T^CATGA
3161 TCATGA
SapI 901 GAAGAGC GCTCTTC(1/4)
49 GCTCTTC
SmaI 1330 CCCGGG CCC^GGG
2000 CCCGGG
SmlI 2965 CTTAAG C^T[CT][GA]AG
3313 CTCAAG
Tth111I 1816 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1936 GACGTCGTC
XmaCI 1330 CCCGGG C^CCGGG
2000 CCCGGG
XmaI 1330 CCCGGG C^CCGGG
2000 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AatII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstEII BstF5I BstNI
BstPI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII