################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AclI AcsI AgeI ApoI AscI AseI AsnI AvrII BcgI BglII BlnI BsiWI BspLU11I BssHII Bst1107I BstAPI BstBI BstXI BstZ17I CpoI DraI EcoRI EcoT22I FseI HincII HindII HpaI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Van91I XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 2350 CGCG CG^CG AccIII 1568 TCCGGA T^CCGGA AflII 2965 CTTAAG C^TTAAG AflIII 2013 ACGTGT A^C[GA][CT]GT AhdI 2914 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 750 CCTGAGG CC^T[ACGT]AGG Aor51HI 2735 AGCGCT AGC^GCT Asp700I 2373 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2914 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2120 TGGCCA TGG^CCA BamHI 1527 GGATCC G^GATCC BbeI 523 GGCGCC GGCGC^C BbrPI 2277 CACGTG CAC^GTG BclI 2852 TGATCA T^GATCA BfrI 2965 CTTAAG C^TTAAG BglI 2653 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1875 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1568 TCCGGA T^CCGGA BsmI 2373 GAATGC GAATGC(1/-1) BspDI 3229 ATCGAT AT^CGAT BspEI 1568 TCCGGA T^CCGGA BsrBI 2106 CCGCTC CCGCTC(-3/-3) BstUI 2350 CGCG CG^CG Bsu36I 750 CCTGAGG CC^T[ACGT]AGG ClaI 3229 ATCGAT AT^CGAT DrdI 3458 GACGGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2914 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2735 AGCGCT AGC^GCT Eco81I 750 CCTGAGG CC^T[ACGT]AGG EcoRV 2320 GATATC GAT^ATC FbaI 2852 TGATCA T^GATCA KasI 523 GGCGCC G^GCGCC KspI 2349 CCGCGG CCGC^GG MamI 1875 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2120 TGGCCA TGG^CCA MroI 1568 TCCGGA T^CCGGA MscI 2120 TGGCCA TGG^CCA MvnI 2350 CGCG CG^CG NarI 523 GGCGCC GG^CGCC NcoI 1475 CCATGG C^CATGG PmaCI 2277 CACGTG CAC^GTG PmlI 2277 CACGTG CAC^GTG SacI 2947 GAGCTC GAGCT^C SacII 2349 CCGCGG CCGC^GG SexAI 1817 ACCAGGT A^CC[AT]GGT SfoI 523 GGCGCC GGC^GCC StuI 2651 AGGCCT AGG^CCT XbaI 94 TCTAGA T^CTAGA XmnI 2373 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 380 GGTACC G^GTACC 1208 GGTACC AccI 637 GTCTAC GT^[AC][GT]AC 3235 GTCTAC Alw44I 2280 GTGCAC G^TGCAC 2713 GTGCAC ApaI 147 GGGCCC GGGCC^C 1545 GGGCCC ApaLI 2280 GTGCAC G^TGCAC 2713 GTGCAC Asp718I 380 GGTACC G^GTACC 1208 GGTACC AspI 1816 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1936 GACGTCGTC AviII 785 TGCGCA TGC^GCA 2222 TGCGCA BfaI 41 CTAG C^TAG 95 CTAG BlpI 2086 GCTAAGC GC^T[ACGT]AGC 3581 GCTAAGC Bpu1102I 2086 GCTAAGC GC^T[ACGT]AGC 3581 GCTAAGC BsaAI 2012 TACGTG [CT]AC^GT[GA] 2277 CACGTG BsmBI 2215 GAGACG CGTCTC(1/5) 3327 GAGACG BspHI 1073 TCATGA T^CATGA 3161 TCATGA CelII 2086 GCTAAGC GC^T[ACGT]AGC 3581 GCTAAGC EagI 1200 CGGCCG C^GGCCG 2697 CGGCCG EclXI 1200 CGGCCG C^GGCCG 2697 CGGCCG Eco52I 1200 CGGCCG C^GGCCG 2697 CGGCCG FspI 785 TGCGCA TGC^GCA 2222 TGCGCA HgaI 1767 GACGC GACGC(5/10) 2217 GACGC HindIII 820 AAGCTT A^AGCTT 3649 AAGCTT KpnI 380 GGTACC GGTAC^C 1208 GGTACC MaeI 41 CTAG C^TAG 95 CTAG PflFI 1816 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1936 GACGTCGTC RcaI 1073 TCATGA T^CATGA 3161 TCATGA SapI 901 GAAGAGC GCTCTTC(1/4) 49 GCTCTTC SmaI 1330 CCCGGG CCC^GGG 2000 CCCGGG SmlI 2965 CTTAAG C^T[CT][GA]AG 3313 CTCAAG Tth111I 1816 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1936 GACGTCGTC XmaCI 1330 CCCGGG C^CCGGG 2000 CCCGGG XmaI 1330 CCCGGG C^CCGGG 2000 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AatII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BciVI BcnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII