##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII Alw44I ApaLI ApoI
Asp700I Asp718I AviII BbvCI BcgI
BclI BfrI BglII BsaBI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
BsrDI Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EcoNI EcoRI EcoRV
FbaI FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NspV
PacI PmeI PshAI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AgeI 3325 ACCGGT A^CCGGT
AhdI 998 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 3289 GGCGCGCC GG^CGCGCC
AseI 3311 ATTAAT AT^TAAT
AsnI 3311 ATTAAT AT^TAAT
AspEI 998 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 394 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3445 TGGCCA TGG^CCA
BciVI 2023 GTATCC GTATCC(6/5)
BglI 435 GCCCTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 654 CGGCCG CG[GA][CT]^CG
BsmBI 1663 GAGACG CGTCTC(1/5)
BsmI 2500 GAATGC GAATGC(1/-1)
Bsp1407I 885 TGTACA T^GTACA
BsrBI 342 GAGCGG CCGCTC(-3/-3)
BsrGI 885 TGTACA T^GTACA
BstAPI 3084 GCAGACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 2774 TTTAAA TTT^AAA
DraIII 1211 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 654 CGGCCG C^GGCCG
Eam1105I 998 GACTGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 797 GAAGAG CTCTTC(1/4)
EclXI 654 CGGCCG C^GGCCG
Eco52I 654 CGGCCG C^GGCCG
EcoT22I 2502 ATGCAT ATGCA^T
HincII 1456 GTCAAC GT[CT]^[GA]AC
HindII 1456 GTCAAC GT[CT]^[GA]AC
Ksp632I 797 GAAGAG CTCTTC(1/4)
KspI 1764 CCGCGG CCGC^GG
MluNI 3445 TGGCCA TGG^CCA
MscI 3445 TGGCCA TGG^CCA
NaeI 1728 GCCGGC GCC^GGC
NgoMIV 1728 GCCGGC G^CCGGC
NruI 1565 TCGCGA TCG^CGA
NsiI 2502 ATGCAT ATGCA^T
NspI 1356 ACATGC [GA]CATG^[CT]
PaeR7I 3162 CTCGAG C^TCGAG
PflFI 394 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3325 ACCGGT A^CCGGT
PshBI 3311 ATTAAT AT^TAAT
PvuII 56 CAGCTG CAG^CTG
SacII 1764 CCGCGG CCGC^GG
SspBI 885 TGTACA T^GTACA
Tth111I 394 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3162 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 886 GTAC GT^AC
1379 GTAC
AflIII 195 ACGTGT A^C[GA][CT]GT
1134 ACGTGT
AlwNI 164 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
3569 CAGTGCCTG
AocI 1082 CCTCAGG CC^T[ACGT]AGG
1702 CCTTAGG
AvrII 404 CCTAGG C^CTAGG
2681 CCTAGG
BbrPI 194 CACGTG CAC^GTG
1133 CACGTG
BlnI 404 CCTAGG C^CTAGG
2681 CCTAGG
BlpI 2012 GCTGAGC GC^T[ACGT]AGC
2147 GCTCAGC
Bpu1102I 2012 GCTGAGC GC^T[ACGT]AGC
2147 GCTCAGC
BsaAI 194 CACGTG [CT]AC^GT[GA]
1133 CACGTG
BsgI 69 GTGCAG GTGCAG(16/14)
1433 GTGCAG
BstEII 534 GGTGACC G^GT[ACGT]ACC
1277 GGTGACC
BstPI 534 GGTGACC G^GT[ACGT]ACC
1277 GGTGACC
Bsu36I 1082 CCTCAGG CC^T[ACGT]AGG
1702 CCTTAGG
CelII 2012 GCTGAGC GC^T[ACGT]AGC
2147 GCTCAGC
EaeI 654 CGGCCG [CT]^GGCC[GA]
3445 TGGCCA
Eco81I 1082 CCTCAGG CC^T[ACGT]AGG
1702 CCTTAGG
EcoO65I 534 GGTGACC G^GT[ACGT]ACC
1277 GGTGACC
MslI 1355 CACATGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2945 CATCTCCGTG
NcoI 148 CCATGG C^CATGG
561 CCATGG
PflMI 163 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1132 CCACGTGTTGG
PmaCI 194 CACGTG CAC^GTG
1133 CACGTG
PmlI 194 CACGTG CAC^GTG
1133 CACGTG
PstI 258 CTGCAG CTGCA^G
622 CTGCAG
RsaI 886 GTAC GT^AC
1379 GTAC
SacI 33 GAGCTC GAGCT^C
726 GAGCTC
Van91I 163 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1132 CCACGTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
Aor51HI ApaI AspHI AvaI AvaII
BamHI BanI BanII BbeI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI Eco47III EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PpuMI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI