##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BglI BlnI BsiWI BsmBI BspDI
BspHI BssHII BstBI ClaI CpoI
DraIII DrdI Eam1105I EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MfeI MluI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SphI
Sse8387I SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 613 TCCGGA T^CCGGA
AflII 2284 CTTAAG C^TTAAG
AlwNI 790 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3492 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2105 AGCGCT AGC^GCT
Asp700I 3207 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2089 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 26 GGCGCC GGCGC^C
BfrI 2284 CTTAAG C^TTAAG
BglII 2317 AGATCT A^GATCT
BlpI 2804 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2804 GCTTAGC GC^T[ACGT]AGC
BsaAI 1190 CACGTA [CT]AC^GT[GA]
BsaBI 3467 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2896 GGTCTC GGTCTC(1/5)
BsaWI 613 TCCGGA [AT]^CCGG[AT]
BseAI 613 TCCGGA T^CCGGA
BsiEI 3172 CGGCCG CG[GA][CT]^CG
Bsp1407I 2252 TGTACA T^GTACA
BspEI 613 TCCGGA T^CCGGA
BspLU11I 2143 ACATGT A^CATGT
BsrBI 66 CCGCTC CCGCTC(-3/-3)
BsrFI 303 ACCGGC [GA]^CCGG[CT]
BsrGI 2252 TGTACA T^GTACA
BsrI 789 CCAGT ACTGG(1/-1)
BssSI 339 CTCGTG CACGAG(-5/-1)
BstEII 713 GGTGACC G^GT[ACGT]ACC
BstPI 713 GGTGACC G^GT[ACGT]ACC
BstXI 148 CCAGAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3492 CCTCAGG CC^T[ACGT]AGG
CelII 2804 GCTTAGC GC^T[ACGT]AGC
Cfr10I 303 ACCGGC [GA]^CCGG[CT]
DraII 51 GGGCCCC [GA]G^G[ACGT]CC[CT]
EagI 3172 CGGCCG C^GGCCG
EclXI 3172 CGGCCG C^GGCCG
Eco47III 2105 AGCGCT AGC^GCT
Eco52I 3172 CGGCCG C^GGCCG
Eco81I 3492 CCTCAGG CC^T[ACGT]AGG
EcoO109I 51 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 713 GGTGACC G^GT[ACGT]ACC
EcoRI 3653 GAATTC G^AATTC
EcoRV 1884 GATATC GAT^ATC
KasI 26 GGCGCC G^GCGCC
MamI 3467 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 613 TCCGGA T^CCGGA
NarI 26 GGCGCC GG^CGCC
NcoI 154 CCATGG C^CATGG
PflFI 2089 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1070 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 3922 GAGCTC GAGCT^C
SfoI 26 GGCGCC GGC^GCC
SpeI 2012 ACTAGT A^CTAGT
SspBI 2252 TGTACA T^GTACA
SspI 1377 AATATT AAT^ATT
Tth111I 2089 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1070 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3207 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 26 GGCGCC G[GA]^CG[CT]C
42 GGCGTC
AflIII 1189 ACACGT A^C[GA][CT]GT
2143 ACATGT
ApaI 51 GGGCCC GGGCC^C
283 GGGCCC
BalI 1489 TGGCCA TGG^CCA
2312 TGGCCA
BbsI 1462 GTCTTC GAAGAC(2/6)
3244 GTCTTC
BfaI 2013 CTAG C^TAG
2181 CTAG
BpmI 1106 CTGGAG CTGGAG(16/14)
2861 CTGGAG
BpuAI 1462 GTCTTC GAAGAC(2/6)
3244 GTCTTC
BsaHI 26 GGCGCC G[GA]^CG[CT]C
42 GGCGTC
BsmFI 2002 GGGAC GGGAC(10/14)
2648 GGGAC
BspMI 217 ACCTGC ACCTGC(4/8)
2060 ACCTGC
Bst1107I 455 GTATAC GTA^TAC
3876 GTATAC
BstZ17I 455 GTATAC GTA^TAC
3876 GTATAC
DraI 3077 TTTAAA TTT^AAA
3092 TTTAAA
DsaI 38 CCGTGG C^C[GA][CT]GG
154 CCATGG
EcoNI 704 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2250 CCTGTACAAGG
Hin1I 26 GGCGCC G[GA]^CG[CT]C
42 GGCGTC
HincII 429 GTCAAC GT[CT]^[GA]AC
825 GTTGAC
HindII 429 GTCAAC GT[CT]^[GA]AC
825 GTTGAC
MaeI 2013 CTAG C^TAG
2181 CTAG
MluNI 1489 TGGCCA TGG^CCA
2312 TGGCCA
MscI 1489 TGGCCA TGG^CCA
2312 TGGCCA
NspI 2143 ACATGT [GA]CATG^[CT]
2186 GCATGT
PvuII 1071 CAGCTG CAG^CTG
2798 CAGCTG
SfcI 1207 CTACAG C^T[GA][CT]AG
2705 CTACAG
SmaI 55 CCCGGG CCC^GGG
2507 CCCGGG
SmlI 2284 CTTAAG C^T[CT][GA]AG
3638 CTTGAG
StuI 335 AGGCCT AGG^CCT
1085 AGGCCT
TaqI 1496 TCGA T^CGA
1717 TCGA
TthHB8I 1496 TCGA T^CGA
1717 TCGA
XmaCI 55 CCCGGG C^CCGGG
2507 CCCGGG
XmaI 55 CCCGGG C^CCGGG
2507 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmI BsoBI
Bsp1286I BsrDI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeI NdeII NlaIII NlaIV PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII