##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AocI Aor51HI AscI Asp718I
AspI AvrII BamHI BbeI BbrPI
BbvCI BciVI BclI BfrI BlnI
BsaAI BsaHI BsaWI BseAI BsiWI
BspEI BssSI BstEII BstPI Bsu36I
CpoI DrdI Eco47III Eco81I EcoNI
EcoO65I FbaI FseI Hin1I KasI
KpnI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NruI
PacI PflFI PinAI PmaCI PmeI
PmlI RsrII SacI SalI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I StuI Tth111I
XcmI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AflIII 3070 ACATGT A^C[GA][CT]GT
AhdI 1900 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3043 ATTAAT AT^TAAT
AsnI 3043 ATTAAT AT^TAAT
AspEI 1900 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 3175 GCAGATATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2497 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 318 AGATCT A^GATCT
BlpI 846 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 846 GCTTAGC GC^T[ACGT]AGC
BsaBI 3305 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3740 GGTCTC GGTCTC(1/5)
BsmBI 2350 CGTCTC CGTCTC(1/5)
BsmI 553 GAATGC GAATGC(1/-1)
BspDI 3312 ATCGAT AT^CGAT
BspLU11I 3070 ACATGT A^CATGT
BsrDI 3424 CATTGC GCAATG(2/0)
BsrFI 1867 GCCGGT [GA]^CCGG[CT]
Bst1107I 3479 GTATAC GTA^TAC
BstBI 888 TTCGAA TT^CGAA
BstZ17I 3479 GTATAC GTA^TAC
CelII 846 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1867 GCCGGT [GA]^CCGG[CT]
ClaI 3312 ATCGAT AT^CGAT
DraIII 2136 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 87 CGGCCG C^GGCCG
Eam1105I 1900 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 87 CGGCCG C^GGCCG
Eco52I 87 CGGCCG C^GGCCG
EcoT22I 3408 ATGCAT ATGCA^T
HgaI 1685 GACGC GACGC(5/10)
HpaI 1300 GTTAAC GTT^AAC
MamI 3305 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1491 CATATG CA^TATG
NotI 86 GCGGCCGC GC^GGCCGC
NsiI 3408 ATGCAT ATGCA^T
NspV 888 TTCGAA TT^CGAA
PaeR7I 203 CTCGAG C^TCGAG
PflMI 1917 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3647 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3148 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3043 ATTAAT AT^TAAT
PstI 1059 CTGCAG CTGCA^G
PvuI 3501 CGATCG CGAT^CG
SapI 735 GAAGAGC GCTCTTC(1/4)
ScaI 1575 AGTACT AGT^ACT
SfuI 888 TTCGAA TT^CGAA
SmaI 38 CCCGGG CCC^GGG
SwaI 3821 ATTTAAAT ATTT^AAAT
Van91I 1917 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 203 CTCGAG C^TCGAG
XmaCI 38 CCCGGG C^CCGGG
XmaI 38 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 782 GTAGAC GT^[AC][GT]AC
3479 GTATAC
AclI 1303 AACGTT AA^CGTT
2873 AACGTT
Alw44I 1023 GTGCAC G^TGCAC
3451 GTGCAC
AlwI 2369 GATCC GGATC(4/5)
1110 GGATC
AlwNI 719 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3378 CAGGAACTG
ApaLI 1023 GTGCAC G^TGCAC
3451 GTGCAC
AvaI 38 CCCGGG C^[CT]CG[GA]G
203 CTCGAG
AviII 726 TGCGCA TGC^GCA
2551 TGCGCA
BalI 2077 TGGCCA TGG^CCA
2901 TGGCCA
BanI 1913 GGCACC G^G[CT][GA]CC
1982 GGCACC
BsiEI 87 CGGCCG CG[GA][CT]^CG
3501 CGATCG
BsoBI 38 CCCGGG C^[CT]CG[GA]G
203 CTCGAG
Bsp1407I 870 TGTACA T^GTACA
1855 TGTACA
BspMI 106 ACCTGC ACCTGC(4/8)
1103 GCAGGT
BsrBI 2625 CCGCTC CCGCTC(-3/-3)
99 GAGCGG
BsrGI 870 TGTACA T^GTACA
1855 TGTACA
BstAPI 1914 GCACCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2001 GCAAGATTTGC
BstXI 1099 CCACGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3137 CCATTTATATGG
BstYI 318 AGATCT [GA]^GATC[CT]
2368 AGATCC
EcoRV 1629 GATATC GAT^ATC
3178 GATATC
FspI 726 TGCGCA TGC^GCA
2551 TGCGCA
HaeII 515 AGCGCC [GA]GCGC^[CT]
2850 GGCGCT
HincII 1300 GTTAAC GT[CT]^[GA]AC
3057 GTTGAC
HindII 1300 GTTAAC GT[CT]^[GA]AC
3057 GTTGAC
KspI 49 CCGCGG CCGC^GG
501 CCGCGG
MflI 318 AGATCT [GA]^GATC[CT]
2368 AGATCC
MluNI 2077 TGGCCA TGG^CCA
2901 TGGCCA
MscI 2077 TGGCCA TGG^CCA
2901 TGGCCA
NcoI 615 CCATGG C^CATGG
2080 CCATGG
PleI 528 GACTC GAGTC(4/5)
651 GAGTC
Psp1406I 1303 AACGTT AA^CGTT
2873 AACGTT
PvuII 719 CAGCTG CAG^CTG
3760 CAGCTG
SacII 49 CCGCGG CCGC^GG
501 CCGCGG
SspBI 870 TGTACA T^GTACA
1855 TGTACA
XbaI 2354 TCTAGA T^CTAGA
3616 TCTAGA
XhoII 318 AGATCT [GA]^GATC[CT]
2368 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApaI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI