##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccI AgeI Aor51HI AscI
AseI AsnI Asp700I AviII AvrII
BamHI BbeI BbvCI BlnI BsiWI
BspDI BspHI BspLU11I BsrDI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII Eco47III EcoNI EcoRV EcoT22I
FseI FspI HpaI KasI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PinAI PmeI PshAI PshBI
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1326 GGTACC G^GTACC
AclI 3179 AACGTT AA^CGTT
AflII 3220 CTTAAG C^TTAAG
AflIII 3705 ACACGT A^C[GA][CT]GT
AhdI 1828 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 370 GTGCAC G^TGCAC
ApaLI 370 GTGCAC G^TGCAC
Asp718I 1326 GGTACC G^GTACC
AspEI 1828 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 3900 GTATCC GTATCC(6/5)
BclI 92 TGATCA T^GATCA
BfrI 3220 CTTAAG C^TTAAG
BglI 1039 GCCCCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 572 AGATCT A^GATCT
BsaBI 1860 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4273 CGTCTC CGTCTC(1/5)
BsmI 2297 GCATTC GAATGC(1/-1)
BstAPI 1903 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 202 GGTTACC G^GT[ACGT]ACC
BstPI 202 GGTTACC G^GT[ACGT]ACC
BstYI 572 AGATCT [GA]^GATC[CT]
DrdI 4031 GACTCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4172 CGGCCG C^GGCCG
Eam1105I 1828 GACTGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4172 CGGCCG C^GGCCG
Eco52I 4172 CGGCCG C^GGCCG
EcoO65I 202 GGTTACC G^GT[ACGT]ACC
EcoRI 544 GAATTC G^AATTC
FbaI 92 TGATCA T^GATCA
HincII 2185 GTTGAC GT[CT]^[GA]AC
HindII 2185 GTTGAC GT[CT]^[GA]AC
HindIII 2163 AAGCTT A^AGCTT
KpnI 1326 GGTACC GGTAC^C
KspI 2023 CCGCGG CCGC^GG
MamI 1860 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 572 AGATCT [GA]^GATC[CT]
NcoI 2058 CCATGG C^CATGG
NruI 4082 TCGCGA TCG^CGA
NspI 3979 GCATGT [GA]CATG^[CT]
PpuMI 2040 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 3179 AACGTT AA^CGTT
SacII 2023 CCGCGG CCGC^GG
SapI 3647 GCTCTTC GCTCTTC(1/4)
SgrAI 325 CACCGGCG C[GA]^CCGG[CT]G
XhoII 572 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 544 GAATTC [GA]^AATT[CT]
1264 AAATTC
AcyI 2785 GACGCC G[GA]^CG[CT]C
4178 GGCGTC
AlwNI 2441 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
3511 CAGGAGCTG
AocI 836 CCTCAGG CC^T[ACGT]AGG
3386 CCTCAGG
ApoI 544 GAATTC [GA]^AATT[CT]
1264 AAATTC
AspI 358 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2883 GACCATGTC
BbrPI 470 CACGTG CAC^GTG
2350 CACGTG
BcgI 1430 GCAGCGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3840 GCACCTGACTCG
BlpI 985 GCTGAGC GC^T[ACGT]AGC
2933 GCTCAGC
Bpu1102I 985 GCTGAGC GC^T[ACGT]AGC
2933 GCTCAGC
BsaHI 2785 GACGCC G[GA]^CG[CT]C
4178 GGCGTC
BsiEI 444 CGACCG CG[GA][CT]^CG
4172 CGGCCG
Bsp1407I 4071 TGTACA T^GTACA
4118 TGTACA
BsrGI 4071 TGTACA T^GTACA
4118 TGTACA
Bsu36I 836 CCTCAGG CC^T[ACGT]AGG
3386 CCTCAGG
CelII 985 GCTGAGC GC^T[ACGT]AGC
2933 GCTCAGC
Eco81I 836 CCTCAGG CC^T[ACGT]AGG
3386 CCTCAGG
Hin1I 2785 GACGCC G[GA]^CG[CT]C
4178 GGCGTC
PflFI 358 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2883 GACCATGTC
PmaCI 470 CACGTG CAC^GTG
2350 CACGTG
PmlI 470 CACGTG CAC^GTG
2350 CACGTG
SspBI 4071 TGTACA T^GTACA
4118 TGTACA
Tth111I 358 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2883 GACCATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PaeR7I PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI