################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## Acc65I AccIII AclI AcsI AflIII Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BbeI BcgI BciVI BclI BlnI BseAI BsiWI BsmBI BspEI BspHI BspLU11I BsrBI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CpoI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I RcaI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SphI Sse8387I StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 532 GACGTC GACGT^C AcyI 532 GACGTC G[GA]^CG[CT]C AgeI 736 ACCGGT A^CCGGT Alw44I 822 GTGCAC G^TGCAC AocI 1579 CCTGAGG CC^T[ACGT]AGG ApaI 1999 GGGCCC GGGCC^C ApaLI 822 GTGCAC G^TGCAC BamHI 90 GGATCC G^GATCC BanI 552 GGTGCC G^G[CT][GA]CC BbvCI 2292 CCTCAGC CCTCAGC(-5/-2) BglII 2675 AGATCT A^GATCT BsaHI 532 GACGTC G[GA]^CG[CT]C BsaWI 736 ACCGGT [AT]^CCGG[AT] BsiEI 918 CGATCG CG[GA][CT]^CG Bsp1407I 2798 TGTACA T^GTACA BspDI 515 ATCGAT AT^CGAT BspMI 1808 GCAGGT ACCTGC(4/8) BsrDI 792 CATTGC GCAATG(2/0) BsrFI 736 ACCGGT [GA]^CCGG[CT] BsrGI 2798 TGTACA T^GTACA BssSI 2664 CTCGTG CACGAG(-5/-1) Bsu36I 1579 CCTGAGG CC^T[ACGT]AGG CfoI 2303 GCGC GCG^C Cfr10I 736 ACCGGT [GA]^CCGG[CT] ClaI 515 ATCGAT AT^CGAT DraI 1257 TTTAAA TTT^AAA DrdI 1803 GACTTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1579 CCTGAGG CC^T[ACGT]AGG HgaI 417 GACGC GACGC(5/10) HhaI 2303 GCGC GCG^C Hin1I 532 GACGTC G[GA]^CG[CT]C HinP1I 2303 GCGC G^CGC PinAI 736 ACCGGT A^CCGGT PvuI 918 CGATCG CGAT^CG ScaI 1055 AGTACT AGT^ACT SexAI 482 ACCTGGT A^CC[AT]GGT SnaBI 2936 TACGTA TAC^GTA SpeI 2892 ACTAGT A^CTAGT SspBI 2798 TGTACA T^GTACA SspI 2924 AATATT AAT^ATT XcmI 275 CCAAGAAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 772 GTCTAC GT^[AC][GT]AC 2145 GTCTAC AccII 60 CGCG CG^CG 1510 CGCG AflII 1988 CTTAAG C^TTAAG 2045 CTTAAG AhdI 1286 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2858 GACTCAGTGTC AlwNI 478 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 2529 CAGGGCCTG AspEI 1286 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2858 GACTCAGTGTC BfrI 1988 CTTAAG C^TTAAG 2045 CTTAAG BglI 1839 GCCCACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2054 GCCACGTGGGC BlpI 75 GCTGAGC GC^T[ACGT]AGC 504 GCTCAGC Bpu1102I 75 GCTGAGC GC^T[ACGT]AGC 504 GCTCAGC BsgI 2377 CTGCAC GTGCAG(16/14) 2389 GTGCAG BstAPI 2391 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2400 GCACACTGTGC BstUI 60 CGCG CG^CG 1510 CGCG CelII 75 GCTGAGC GC^T[ACGT]AGC 504 GCTCAGC Eam1105I 1286 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2858 GACTCAGTGTC EcoT14I 2625 CCATGG C^C[AT][AT]GG 2912 CCATGG MslI 357 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2264 CATGTGAATG MvnI 60 CGCG CG^CG 1510 CGCG NcoI 2625 CCATGG C^CATGG 2912 CCATGG NspI 1324 GCATGT [GA]CATG^[CT] 2079 ACATGC PflMI 556 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2872 CCATCAGCTGG PpuMI 479 AGGACCT [GA]G^G[AT]CC[CT] 1772 GGGACCT SapI 1862 GCTCTTC GCTCTTC(1/4) 2838 GCTCTTC StyI 2625 CCATGG C^C[AT][AT]GG 2912 CCATGG TfiI 518 GATTC G^A[AT]TC 2681 GAATC Van91I 556 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2872 CCATCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbrPI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaBI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DraIII DsaI EarI EcoO109I EcoRII EcoRV FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PmaCI PmlI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII