##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflIII
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BbeI BcgI BciVI BclI
BlnI BseAI BsiWI BsmBI BspEI
BspHI BspLU11I BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CpoI EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SphI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 532 GACGTC GACGT^C
AcyI 532 GACGTC G[GA]^CG[CT]C
AgeI 736 ACCGGT A^CCGGT
Alw44I 822 GTGCAC G^TGCAC
AocI 1579 CCTGAGG CC^T[ACGT]AGG
ApaI 1999 GGGCCC GGGCC^C
ApaLI 822 GTGCAC G^TGCAC
BamHI 90 GGATCC G^GATCC
BanI 552 GGTGCC G^G[CT][GA]CC
BbvCI 2292 CCTCAGC CCTCAGC(-5/-2)
BglII 2675 AGATCT A^GATCT
BsaHI 532 GACGTC G[GA]^CG[CT]C
BsaWI 736 ACCGGT [AT]^CCGG[AT]
BsiEI 918 CGATCG CG[GA][CT]^CG
Bsp1407I 2798 TGTACA T^GTACA
BspDI 515 ATCGAT AT^CGAT
BspMI 1808 GCAGGT ACCTGC(4/8)
BsrDI 792 CATTGC GCAATG(2/0)
BsrFI 736 ACCGGT [GA]^CCGG[CT]
BsrGI 2798 TGTACA T^GTACA
BssSI 2664 CTCGTG CACGAG(-5/-1)
Bsu36I 1579 CCTGAGG CC^T[ACGT]AGG
CfoI 2303 GCGC GCG^C
Cfr10I 736 ACCGGT [GA]^CCGG[CT]
ClaI 515 ATCGAT AT^CGAT
DraI 1257 TTTAAA TTT^AAA
DrdI 1803 GACTTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1579 CCTGAGG CC^T[ACGT]AGG
HgaI 417 GACGC GACGC(5/10)
HhaI 2303 GCGC GCG^C
Hin1I 532 GACGTC G[GA]^CG[CT]C
HinP1I 2303 GCGC G^CGC
PinAI 736 ACCGGT A^CCGGT
PvuI 918 CGATCG CGAT^CG
ScaI 1055 AGTACT AGT^ACT
SexAI 482 ACCTGGT A^CC[AT]GGT
SnaBI 2936 TACGTA TAC^GTA
SpeI 2892 ACTAGT A^CTAGT
SspBI 2798 TGTACA T^GTACA
SspI 2924 AATATT AAT^ATT
XcmI 275 CCAAGAAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 772 GTCTAC GT^[AC][GT]AC
2145 GTCTAC
AccII 60 CGCG CG^CG
1510 CGCG
AflII 1988 CTTAAG C^TTAAG
2045 CTTAAG
AhdI 1286 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2858 GACTCAGTGTC
AlwNI 478 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
2529 CAGGGCCTG
AspEI 1286 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2858 GACTCAGTGTC
BfrI 1988 CTTAAG C^TTAAG
2045 CTTAAG
BglI 1839 GCCCACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2054 GCCACGTGGGC
BlpI 75 GCTGAGC GC^T[ACGT]AGC
504 GCTCAGC
Bpu1102I 75 GCTGAGC GC^T[ACGT]AGC
504 GCTCAGC
BsgI 2377 CTGCAC GTGCAG(16/14)
2389 GTGCAG
BstAPI 2391 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2400 GCACACTGTGC
BstUI 60 CGCG CG^CG
1510 CGCG
CelII 75 GCTGAGC GC^T[ACGT]AGC
504 GCTCAGC
Eam1105I 1286 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2858 GACTCAGTGTC
EcoT14I 2625 CCATGG C^C[AT][AT]GG
2912 CCATGG
MslI 357 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2264 CATGTGAATG
MvnI 60 CGCG CG^CG
1510 CGCG
NcoI 2625 CCATGG C^CATGG
2912 CCATGG
NspI 1324 GCATGT [GA]CATG^[CT]
2079 ACATGC
PflMI 556 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2872 CCATCAGCTGG
PpuMI 479 AGGACCT [GA]G^G[AT]CC[CT]
1772 GGGACCT
SapI 1862 GCTCTTC GCTCTTC(1/4)
2838 GCTCTTC
StyI 2625 CCATGG C^C[AT][AT]GG
2912 CCATGG
TfiI 518 GATTC G^A[AT]TC
2681 GAATC
Van91I 556 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2872 CCATCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbrPI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaBI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DraIII
DsaI EarI EcoO109I EcoRII EcoRV
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PmaCI PmlI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII