################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AccIII AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI AspEI AspI BamHI BciVI BclI BfrI BglII BsaBI BsaWI BseAI BsiWI BsmBI BsmI BspEI BspHI BspLU11I BssSI Bst1107I BstBI BstYI BstZ17I CpoI DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KspI MamI MfeI MflI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoII ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1395 GACGTC GACGT^C Acc65I 2755 GGTACC G^GTACC Alw44I 2406 GTGCAC G^TGCAC Aor51HI 1523 AGCGCT AGC^GCT ApaLI 2406 GTGCAC G^TGCAC Asp718I 2755 GGTACC G^GTACC AviII 409 TGCGCA TGC^GCA AvrII 2645 CCTAGG C^CTAGG BbvCI 2715 GCTGAGG CCTCAGC(-5/-2) BlnI 2645 CCTAGG C^CTAGG BlpI 704 GCTGAGC GC^T[ACGT]AGC Bpu1102I 704 GCTGAGC GC^T[ACGT]AGC BsiEI 641 CGACCG CG[GA][CT]^CG Bsp1407I 1016 TGTACA T^GTACA BspDI 2376 ATCGAT AT^CGAT BsrBI 148 GAGCGG CCGCTC(-3/-3) BsrGI 1016 TGTACA T^GTACA BstAPI 1470 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1721 GGTCACC G^GT[ACGT]ACC BstPI 1721 GGTCACC G^GT[ACGT]ACC BstXI 1039 CCACGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 704 GCTGAGC GC^T[ACGT]AGC ClaI 2376 ATCGAT AT^CGAT Eco47III 1523 AGCGCT AGC^GCT EcoNI 2097 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1721 GGTCACC G^GT[ACGT]ACC FspI 409 TGCGCA TGC^GCA HincII 2592 GTCAAC GT[CT]^[GA]AC HindII 2592 GTCAAC GT[CT]^[GA]AC KpnI 2755 GGTACC GGTAC^C MseI 1098 TTAA T^TAA NaeI 614 GCCGGC GCC^GGC NcoI 1702 CCATGG C^CATGG NgoMIV 614 GCCGGC G^CCGGC PaeR7I 1630 CTCGAG C^TCGAG PshAI 1052 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 175 GAGCTC GAGCT^C SapI 678 GAAGAGC GCTCTTC(1/4) SexAI 2624 ACCAGGT A^CC[AT]GGT SmlI 1630 CTCGAG C^T[CT][GA]AG SspBI 1016 TGTACA T^GTACA StuI 2036 AGGCCT AGG^CCT TfiI 2664 GAATC G^A[AT]TC Tru9I 1098 TTAA T^TAA Tsp509I 1116 AATT ^AATT XhoI 1630 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AlwI 686 GATCC GGATC(4/5) 896 GATCC BbrPI 1020 CACGTG CAC^GTG 1797 CACGTG BbsI 1211 GAAGAC GAAGAC(2/6) 1637 GAAGAC BpuAI 1211 GAAGAC GAAGAC(2/6) 1637 GAAGAC BsrDI 691 CATTGC GCAATG(2/0) 1337 CATTGC BsrFI 614 GCCGGC [GA]^CCGG[CT] 1074 ACCGGC Cfr10I 614 GCCGGC [GA]^CCGG[CT] 1074 ACCGGC DrdI 1214 GACCTTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2294 GACAGCTTCGTC MslI 1316 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2255 CATCGCTATG PmaCI 1020 CACGTG CAC^GTG 1797 CACGTG PmlI 1020 CACGTG CAC^GTG 1797 CACGTG PvuII 1065 CAGCTG CAG^CTG 2507 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI AlwNI AocI ApaI Asp700I AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcgI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssHII BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XmaCI XmaI XmnI