##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
AspEI AspI BamHI BciVI BclI
BfrI BglII BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BspEI BspHI
BspLU11I BssSI Bst1107I BstBI BstYI
BstZ17I CpoI DraI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KspI
MamI MfeI MflI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoII
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1395 GACGTC GACGT^C
Acc65I 2755 GGTACC G^GTACC
Alw44I 2406 GTGCAC G^TGCAC
Aor51HI 1523 AGCGCT AGC^GCT
ApaLI 2406 GTGCAC G^TGCAC
Asp718I 2755 GGTACC G^GTACC
AviII 409 TGCGCA TGC^GCA
AvrII 2645 CCTAGG C^CTAGG
BbvCI 2715 GCTGAGG CCTCAGC(-5/-2)
BlnI 2645 CCTAGG C^CTAGG
BlpI 704 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 704 GCTGAGC GC^T[ACGT]AGC
BsiEI 641 CGACCG CG[GA][CT]^CG
Bsp1407I 1016 TGTACA T^GTACA
BspDI 2376 ATCGAT AT^CGAT
BsrBI 148 GAGCGG CCGCTC(-3/-3)
BsrGI 1016 TGTACA T^GTACA
BstAPI 1470 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1721 GGTCACC G^GT[ACGT]ACC
BstPI 1721 GGTCACC G^GT[ACGT]ACC
BstXI 1039 CCACGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 704 GCTGAGC GC^T[ACGT]AGC
ClaI 2376 ATCGAT AT^CGAT
Eco47III 1523 AGCGCT AGC^GCT
EcoNI 2097 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1721 GGTCACC G^GT[ACGT]ACC
FspI 409 TGCGCA TGC^GCA
HincII 2592 GTCAAC GT[CT]^[GA]AC
HindII 2592 GTCAAC GT[CT]^[GA]AC
KpnI 2755 GGTACC GGTAC^C
MseI 1098 TTAA T^TAA
NaeI 614 GCCGGC GCC^GGC
NcoI 1702 CCATGG C^CATGG
NgoMIV 614 GCCGGC G^CCGGC
PaeR7I 1630 CTCGAG C^TCGAG
PshAI 1052 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 175 GAGCTC GAGCT^C
SapI 678 GAAGAGC GCTCTTC(1/4)
SexAI 2624 ACCAGGT A^CC[AT]GGT
SmlI 1630 CTCGAG C^T[CT][GA]AG
SspBI 1016 TGTACA T^GTACA
StuI 2036 AGGCCT AGG^CCT
TfiI 2664 GAATC G^A[AT]TC
Tru9I 1098 TTAA T^TAA
Tsp509I 1116 AATT ^AATT
XhoI 1630 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 686 GATCC GGATC(4/5)
896 GATCC
BbrPI 1020 CACGTG CAC^GTG
1797 CACGTG
BbsI 1211 GAAGAC GAAGAC(2/6)
1637 GAAGAC
BpuAI 1211 GAAGAC GAAGAC(2/6)
1637 GAAGAC
BsrDI 691 CATTGC GCAATG(2/0)
1337 CATTGC
BsrFI 614 GCCGGC [GA]^CCGG[CT]
1074 ACCGGC
Cfr10I 614 GCCGGC [GA]^CCGG[CT]
1074 ACCGGC
DrdI 1214 GACCTTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2294 GACAGCTTCGTC
MslI 1316 CATCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2255 CATCGCTATG
PmaCI 1020 CACGTG CAC^GTG
1797 CACGTG
PmlI 1020 CACGTG CAC^GTG
1797 CACGTG
PvuII 1065 CAGCTG CAG^CTG
2507 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwNI AocI ApaI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssHII BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI XmnI