##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
BfrI BseAI BsiEI BsiWI BspDI
BspEI BspHI BsrDI Bst1107I BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRI EcoRV KpnI MfeI
MluI MroI MunI NotI NruI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SbfI ScaI SfiI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 537 GGCGCC G[GA]^CG[CT]C
AhdI 2664 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 603 GTGCAC G^TGCAC
AocI 3455 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2370 AGCGCT AGC^GCT
ApaLI 603 GTGCAC G^TGCAC
AspEI 2664 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3857 CCTAGG C^CTAGG
BbeI 537 GGCGCC GGCGC^C
BglI 1332 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3857 CCTAGG C^CTAGG
BsaBI 2660 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 537 GGCGCC G[GA]^CG[CT]C
BsmI 2637 GAATGC GAATGC(1/-1)
BstBI 420 TTCGAA TT^CGAA
Bsu36I 3455 CCTGAGG CC^T[ACGT]AGG
DraI 4169 TTTAAA TTT^AAA
Eam1105I 2664 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2370 AGCGCT AGC^GCT
Eco81I 3455 CCTGAGG CC^T[ACGT]AGG
EcoT22I 4179 ATGCAT ATGCA^T
FseI 286 GGCCGGCC GGCCGG^CC
Hin1I 537 GGCGCC G[GA]^CG[CT]C
HindIII 4392 AAGCTT A^AGCTT
HpaI 3363 GTTAAC GTT^AAC
KasI 537 GGCGCC G^GCGCC
KspI 994 CCGCGG CCGC^GG
MamI 2660 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 537 GGCGCC GG^CGCC
NdeI 4182 CATATG CA^TATG
NheI 4444 GCTAGC G^CTAGC
NsiI 4179 ATGCAT ATGCA^T
NspV 420 TTCGAA TT^CGAA
SacII 994 CCGCGG CCGC^GG
SalI 440 GTCGAC G^TCGAC
SapI 2884 GCTCTTC GCTCTTC(1/4)
SexAI 1787 ACCTGGT A^CC[AT]GGT
SfoI 537 GGCGCC GGC^GCC
SfuI 420 TTCGAA TT^CGAA
SmaI 76 CCCGGG CCC^GGG
SphI 2209 GCATGC GCATG^C
SspI 4144 AATATT AAT^ATT
XmaCI 76 CCCGGG C^CCGGG
XmaI 76 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
ApaI 1862 GGGCCC GGGCC^C
3268 GGGCCC
AspI 42 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
507 GACATCGTC
AviII 356 TGCGCA TGC^GCA
3511 TGCGCA
BalI 986 TGGCCA TGG^CCA
1390 TGGCCA
BbrPI 966 CACGTG CAC^GTG
1586 CACGTG
BbvCI 3441 GCTGAGG CCTCAGC(-5/-2)
4515 GCTGAGG
BcgI 1659 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2315 CGATGACCCTGC
BciVI 3320 GGATAC GTATCC(6/5)
127 GTATCC
BclI 745 TGATCA T^GATCA
3558 TGATCA
BglII 1372 AGATCT A^GATCT
4331 AGATCT
BsaAI 966 CACGTG [CT]AC^GT[GA]
1586 CACGTG
BsaI 2405 GGTCTC GGTCTC(1/5)
3833 GGTCTC
BsaWI 2875 ACCGGA [AT]^CCGG[AT]
4522 ACCGGA
BspLU11I 3661 ACATGT A^CATGT
3944 ACATGT
BsrBI 909 GAGCGG CCGCTC(-3/-3)
1796 GAGCGG
BsrFI 287 GCCGGC [GA]^CCGG[CT]
1360 GCCGGC
BssHII 4031 GCGCGC G^CGCGC
4224 GCGCGC
BstEII 2391 GGTGACC G^GT[ACGT]ACC
3865 GGTGACC
BstPI 2391 GGTGACC G^GT[ACGT]ACC
3865 GGTGACC
Cfr10I 287 GCCGGC [GA]^CCGG[CT]
1360 GCCGGC
EcoO65I 2391 GGTGACC G^GT[ACGT]ACC
3865 GGTGACC
FbaI 745 TGATCA T^GATCA
3558 TGATCA
FspI 356 TGCGCA TGC^GCA
3511 TGCGCA
HincII 440 GTCGAC GT[CT]^[GA]AC
3363 GTTAAC
HindII 440 GTCGAC GT[CT]^[GA]AC
3363 GTTAAC
MluNI 986 TGGCCA TGG^CCA
1390 TGGCCA
MscI 986 TGGCCA TGG^CCA
1390 TGGCCA
NaeI 287 GCCGGC GCC^GGC
1360 GCCGGC
NcoI 790 CCATGG C^CATGG
1516 CCATGG
NgoMIV 287 GCCGGC G^CCGGC
1360 GCCGGC
PflFI 42 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
507 GACATCGTC
PmaCI 966 CACGTG CAC^GTG
1586 CACGTG
PmlI 966 CACGTG CAC^GTG
1586 CACGTG
SmlI 90 CTCAAG C^T[CT][GA]AG
180 CTCAAG
StuI 1343 AGGCCT AGG^CCT
3553 AGGCCT
Tth111I 42 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
507 GACATCGTC
XcmI 209 CCAGCACAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1929 CCAGAAACCAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BamHI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI