################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AscI AseI AsnI Asp718I BfrI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrDI Bst1107I BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I EcoRI EcoRV KpnI MfeI MluI MroI MunI NotI NruI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SbfI ScaI SfiI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcyI 537 GGCGCC G[GA]^CG[CT]C AhdI 2664 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 603 GTGCAC G^TGCAC AocI 3455 CCTGAGG CC^T[ACGT]AGG Aor51HI 2370 AGCGCT AGC^GCT ApaLI 603 GTGCAC G^TGCAC AspEI 2664 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3857 CCTAGG C^CTAGG BbeI 537 GGCGCC GGCGC^C BglI 1332 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3857 CCTAGG C^CTAGG BsaBI 2660 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 537 GGCGCC G[GA]^CG[CT]C BsmI 2637 GAATGC GAATGC(1/-1) BstBI 420 TTCGAA TT^CGAA Bsu36I 3455 CCTGAGG CC^T[ACGT]AGG DraI 4169 TTTAAA TTT^AAA Eam1105I 2664 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2370 AGCGCT AGC^GCT Eco81I 3455 CCTGAGG CC^T[ACGT]AGG EcoT22I 4179 ATGCAT ATGCA^T FseI 286 GGCCGGCC GGCCGG^CC Hin1I 537 GGCGCC G[GA]^CG[CT]C HindIII 4392 AAGCTT A^AGCTT HpaI 3363 GTTAAC GTT^AAC KasI 537 GGCGCC G^GCGCC KspI 994 CCGCGG CCGC^GG MamI 2660 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 537 GGCGCC GG^CGCC NdeI 4182 CATATG CA^TATG NheI 4444 GCTAGC G^CTAGC NsiI 4179 ATGCAT ATGCA^T NspV 420 TTCGAA TT^CGAA SacII 994 CCGCGG CCGC^GG SalI 440 GTCGAC G^TCGAC SapI 2884 GCTCTTC GCTCTTC(1/4) SexAI 1787 ACCTGGT A^CC[AT]GGT SfoI 537 GGCGCC GGC^GCC SfuI 420 TTCGAA TT^CGAA SmaI 76 CCCGGG CCC^GGG SphI 2209 GCATGC GCATG^C SspI 4144 AATATT AAT^ATT XmaCI 76 CCCGGG C^CCGGG XmaI 76 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### ApaI 1862 GGGCCC GGGCC^C 3268 GGGCCC AspI 42 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 507 GACATCGTC AviII 356 TGCGCA TGC^GCA 3511 TGCGCA BalI 986 TGGCCA TGG^CCA 1390 TGGCCA BbrPI 966 CACGTG CAC^GTG 1586 CACGTG BbvCI 3441 GCTGAGG CCTCAGC(-5/-2) 4515 GCTGAGG BcgI 1659 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2315 CGATGACCCTGC BciVI 3320 GGATAC GTATCC(6/5) 127 GTATCC BclI 745 TGATCA T^GATCA 3558 TGATCA BglII 1372 AGATCT A^GATCT 4331 AGATCT BsaAI 966 CACGTG [CT]AC^GT[GA] 1586 CACGTG BsaI 2405 GGTCTC GGTCTC(1/5) 3833 GGTCTC BsaWI 2875 ACCGGA [AT]^CCGG[AT] 4522 ACCGGA BspLU11I 3661 ACATGT A^CATGT 3944 ACATGT BsrBI 909 GAGCGG CCGCTC(-3/-3) 1796 GAGCGG BsrFI 287 GCCGGC [GA]^CCGG[CT] 1360 GCCGGC BssHII 4031 GCGCGC G^CGCGC 4224 GCGCGC BstEII 2391 GGTGACC G^GT[ACGT]ACC 3865 GGTGACC BstPI 2391 GGTGACC G^GT[ACGT]ACC 3865 GGTGACC Cfr10I 287 GCCGGC [GA]^CCGG[CT] 1360 GCCGGC EcoO65I 2391 GGTGACC G^GT[ACGT]ACC 3865 GGTGACC FbaI 745 TGATCA T^GATCA 3558 TGATCA FspI 356 TGCGCA TGC^GCA 3511 TGCGCA HincII 440 GTCGAC GT[CT]^[GA]AC 3363 GTTAAC HindII 440 GTCGAC GT[CT]^[GA]AC 3363 GTTAAC MluNI 986 TGGCCA TGG^CCA 1390 TGGCCA MscI 986 TGGCCA TGG^CCA 1390 TGGCCA NaeI 287 GCCGGC GCC^GGC 1360 GCCGGC NcoI 790 CCATGG C^CATGG 1516 CCATGG NgoMIV 287 GCCGGC G^CCGGC 1360 GCCGGC PflFI 42 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 507 GACATCGTC PmaCI 966 CACGTG CAC^GTG 1586 CACGTG PmlI 966 CACGTG CAC^GTG 1586 CACGTG SmlI 90 CTCAAG C^T[CT][GA]AG 180 CTCAAG StuI 1343 AGGCCT AGG^CCT 3553 AGGCCT Tth111I 42 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 507 GACATCGTC XcmI 209 CCAGCACAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1929 CCAGAAACCAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI