##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AscI        AseI        AsnI        Asp718I     
BfrI        BseAI       BsiEI       BsiWI       BspDI       
BspEI       BspHI       BsrDI       Bst1107I    BstZ17I     
ClaI        CpoI        DrdI        EagI        EclXI       
Eco52I      EcoRI       EcoRV       KpnI        MfeI        
MluI        MroI        MunI        NotI        NruI        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SbfI        ScaI        SfiI        SgrAI       SnaBI       
SpeI        Sse8387I    SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AcyI          537  GGCGCC               G[GA]^CG[CT]C
AhdI         2664  GACATCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        603  GTGCAC               G^TGCAC
AocI         3455  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2370  AGCGCT               AGC^GCT
ApaLI         603  GTGCAC               G^TGCAC
AspEI        2664  GACATCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        3857  CCTAGG               C^CTAGG
BbeI          537  GGCGCC               GGCGC^C
BglI         1332  GCCTGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         3857  CCTAGG               C^CTAGG
BsaBI        2660  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         537  GGCGCC               G[GA]^CG[CT]C
BsmI         2637  GAATGC               GAATGC(1/-1)
BstBI         420  TTCGAA               TT^CGAA
Bsu36I       3455  CCTGAGG              CC^T[ACGT]AGG
DraI         4169  TTTAAA               TTT^AAA
Eam1105I     2664  GACATCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2370  AGCGCT               AGC^GCT
Eco81I       3455  CCTGAGG              CC^T[ACGT]AGG
EcoT22I      4179  ATGCAT               ATGCA^T
FseI          286  GGCCGGCC             GGCCGG^CC
Hin1I         537  GGCGCC               G[GA]^CG[CT]C
HindIII      4392  AAGCTT               A^AGCTT
HpaI         3363  GTTAAC               GTT^AAC
KasI          537  GGCGCC               G^GCGCC
KspI          994  CCGCGG               CCGC^GG
MamI         2660  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI          537  GGCGCC               GG^CGCC
NdeI         4182  CATATG               CA^TATG
NheI         4444  GCTAGC               G^CTAGC
NsiI         4179  ATGCAT               ATGCA^T
NspV          420  TTCGAA               TT^CGAA
SacII         994  CCGCGG               CCGC^GG
SalI          440  GTCGAC               G^TCGAC
SapI         2884  GCTCTTC              GCTCTTC(1/4)
SexAI        1787  ACCTGGT              A^CC[AT]GGT
SfoI          537  GGCGCC               GGC^GCC
SfuI          420  TTCGAA               TT^CGAA
SmaI           76  CCCGGG               CCC^GGG
SphI         2209  GCATGC               GCATG^C
SspI         4144  AATATT               AAT^ATT
XmaCI          76  CCCGGG               C^CCGGG
XmaI           76  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

ApaI         1862  GGGCCC               GGGCC^C
             3268  GGGCCC              
AspI           42  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              507  GACATCGTC           
AviII         356  TGCGCA               TGC^GCA
             3511  TGCGCA              
BalI          986  TGGCCA               TGG^CCA
             1390  TGGCCA              
BbrPI         966  CACGTG               CAC^GTG
             1586  CACGTG              
BbvCI        3441  GCTGAGG              CCTCAGC(-5/-2)
             4515  GCTGAGG             
BcgI         1659  CGACTCCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2315  CGATGACCCTGC        
BciVI        3320  GGATAC               GTATCC(6/5)
              127  GTATCC              
BclI          745  TGATCA               T^GATCA
             3558  TGATCA              
BglII        1372  AGATCT               A^GATCT
             4331  AGATCT              
BsaAI         966  CACGTG               [CT]AC^GT[GA]
             1586  CACGTG              
BsaI         2405  GGTCTC               GGTCTC(1/5)
             3833  GGTCTC              
BsaWI        2875  ACCGGA               [AT]^CCGG[AT]
             4522  ACCGGA              
BspLU11I     3661  ACATGT               A^CATGT
             3944  ACATGT              
BsrBI         909  GAGCGG               CCGCTC(-3/-3)
             1796  GAGCGG              
BsrFI         287  GCCGGC               [GA]^CCGG[CT]
             1360  GCCGGC              
BssHII       4031  GCGCGC               G^CGCGC
             4224  GCGCGC              
BstEII       2391  GGTGACC              G^GT[ACGT]ACC
             3865  GGTGACC             
BstPI        2391  GGTGACC              G^GT[ACGT]ACC
             3865  GGTGACC             
Cfr10I        287  GCCGGC               [GA]^CCGG[CT]
             1360  GCCGGC              
EcoO65I      2391  GGTGACC              G^GT[ACGT]ACC
             3865  GGTGACC             
FbaI          745  TGATCA               T^GATCA
             3558  TGATCA              
FspI          356  TGCGCA               TGC^GCA
             3511  TGCGCA              
HincII        440  GTCGAC               GT[CT]^[GA]AC
             3363  GTTAAC              
HindII        440  GTCGAC               GT[CT]^[GA]AC
             3363  GTTAAC              
MluNI         986  TGGCCA               TGG^CCA
             1390  TGGCCA              
MscI          986  TGGCCA               TGG^CCA
             1390  TGGCCA              
NaeI          287  GCCGGC               GCC^GGC
             1360  GCCGGC              
NcoI          790  CCATGG               C^CATGG
             1516  CCATGG              
NgoMIV        287  GCCGGC               G^CCGGC
             1360  GCCGGC              
PflFI          42  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              507  GACATCGTC           
PmaCI         966  CACGTG               CAC^GTG
             1586  CACGTG              
PmlI          966  CACGTG               CAC^GTG
             1586  CACGTG              
SmlI           90  CTCAAG               C^T[CT][GA]AG
              180  CTCAAG              
StuI         1343  AGGCCT               AGG^CCT
             3553  AGGCCT              
Tth111I        42  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              507  GACATCGTC           
XcmI          209  CCAGCACAAAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1929  CCAGAAACCAAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaI        AvaII       BamHI       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspMI       BsrGI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspBI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI