##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BbeI BcgI
BciVI BlnI BlpI BpmI Bpu1102I
BsaHI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI FseI FspI HaeII Hin1I
HpaI KasI KpnI KspI MluI
MluNI MroI MscI NarI NheI
NotI NruI PacI PaeR7I PflMI
PmeI PpuMI PshAI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 70 GTAGAC GT^[AC][GT]AC
AccII 3367 CGCG CG^CG
AflII 791 CTTAAG C^TTAAG
AgeI 1028 ACCGGT A^CCGGT
AlwNI 3195 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 2091 GGATCC G^GATCC
BanI 2549 GGCACC G^G[CT][GA]CC
BanII 1333 GAGCTC G[GA]GC[CT]^C
BbrPI 2885 CACGTG CAC^GTG
BbvCI 1514 GCTGAGG CCTCAGC(-5/-2)
BfrI 791 CTTAAG C^TTAAG
BglI 601 GCCGACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1659 AGATCT A^GATCT
BsaBI 1407 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2941 GGTCTC GGTCTC(1/5)
BsaWI 1028 ACCGGT [AT]^CCGG[AT]
Bsp1407I 2881 TGTACA T^GTACA
BspLU11I 1689 ACATGT A^CATGT
BspMI 2028 ACCTGC ACCTGC(4/8)
BsrDI 1738 CATTGC GCAATG(2/0)
BsrGI 2881 TGTACA T^GTACA
BstAPI 2337 GCATCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 594 TTCGAA TT^CGAA
BstUI 3367 CGCG CG^CG
CfoI 617 GCGC GCG^C
DraII 779 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 267 GACAGTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1108 CCATGG C^C[GA][CT]GG
EaeI 892 CGGCCA [CT]^GGCC[GA]
EcoO109I 779 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 702 GATATC GAT^ATC
HgaI 905 GACGC GACGC(5/10)
HhaI 617 GCGC GCG^C
HinP1I 617 GCGC G^CGC
HincII 1482 GTTGAC GT[CT]^[GA]AC
HindII 1482 GTTGAC GT[CT]^[GA]AC
MamI 1407 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3596 CAATTG C^AATTG
MunI 3596 CAATTG C^AATTG
MvnI 3367 CGCG CG^CG
NaeI 408 GCCGGC GCC^GGC
NcoI 1108 CCATGG C^CATGG
NgoMIV 408 GCCGGC G^CCGGC
NspV 594 TTCGAA TT^CGAA
PinAI 1028 ACCGGT A^CCGGT
PmaCI 2885 CACGTG CAC^GTG
PmlI 2885 CACGTG CAC^GTG
PvuII 1512 CAGCTG CAG^CTG
SacI 1333 GAGCTC GAGCT^C
SapI 865 GCTCTTC GCTCTTC(1/4)
ScaI 3644 AGTACT AGT^ACT
SexAI 3234 ACCTGGT A^CC[AT]GGT
SfuI 594 TTCGAA TT^CGAA
SspBI 2881 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AocI 775 CCTCAGG CC^T[ACGT]AGG
3805 CCTGAGG
AseI 3677 ATTAAT AT^TAAT
3739 ATTAAT
AsnI 3677 ATTAAT AT^TAAT
3739 ATTAAT
AspI 297 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1471 GACTGAGTC
AvaII 1144 GGACC G^G[AT]CC
1344 GGACC
BclI 1418 TGATCA T^GATCA
1605 TGATCA
BcnI 331 CCGGG CC^[GC]GG
2619 CCCGG
BseRI 2207 GAGGAG GAGGAG(10/8)
2210 GAGGAG
BsiYI 729 CCCCAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
902 CCAGACGCAGG
BslI 729 CCCCAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
902 CCAGACGCAGG
BsmBI 996 GAGACG CGTCTC(1/5)
1286 GAGACG
BsmI 2333 GAATGC GAATGC(1/-1)
2358 GAATGC
BspHI 2751 TCATGA T^CATGA
3035 TCATGA
BsrFI 408 GCCGGC [GA]^CCGG[CT]
1028 ACCGGT
BstXI 1573 CCAGACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2347 CCAAACTGTTGG
BstYI 1659 AGATCT [GA]^GATC[CT]
2091 GGATCC
Bsu36I 775 CCTCAGG CC^T[ACGT]AGG
3805 CCTGAGG
Cfr10I 408 GCCGGC [GA]^CCGG[CT]
1028 ACCGGT
Eco81I 775 CCTCAGG CC^T[ACGT]AGG
3805 CCTGAGG
EcoT22I 550 ATGCAT ATGCA^T
2335 ATGCAT
FauI 292 GCGGG CCCGC(4/6)
2546 GCGGG
FbaI 1418 TGATCA T^GATCA
1605 TGATCA
MflI 1659 AGATCT [GA]^GATC[CT]
2091 GGATCC
MspA1I 19 CAGCGG C[AC]G^C[GT]G
1512 CAGCTG
NciI 331 CCGGG CC^[GC]GG
2619 CCCGG
NdeI 2033 CATATG CA^TATG
2690 CATATG
NsiI 550 ATGCAT ATGCA^T
2335 ATGCAT
PflFI 297 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1471 GACTGAGTC
PshBI 3677 ATTAAT AT^TAAT
3739 ATTAAT
RcaI 2751 TCATGA T^CATGA
3035 TCATGA
StuI 645 AGGCCT AGG^CCT
910 AGGCCT
Tth111I 297 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1471 GACTGAGTC
XhoII 1659 AGATCT [GA]^GATC[CT]
2091 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI BbsI BbvI
BfaI BmyI BpuAI BsaAI BsaJI
BsgI BsiHKAI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I