##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI Asp718I AvrII BbeI BbrPI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI ClaI CpoI DraIII
DrdI Eco47III EcoRI FseI KasI
KpnI MamI MfeI MluI MroI
MunI NarI NotI NruI PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SalI ScaI SexAI
SfoI SgrAI SnaBI SphI SspBI
SwaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AflII 3545 CTTAAG C^TTAAG
AhdI 10 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 520 GTGCAC G^TGCAC
ApaI 42 GGGCCC GGGCC^C
ApaLI 520 GTGCAC G^TGCAC
AseI 4295 ATTAAT AT^TAAT
AsnI 4295 ATTAAT AT^TAAT
Asp700I 147 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 10 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4953 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2587 TGCGCA TGC^GCA
BamHI 2497 GGATCC G^GATCC
BanI 672 GGCACC G^G[CT][GA]CC
BbvCI 1718 CCTCAGC CCTCAGC(-5/-2)
BcgI 786 GCAAGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4376 GTATCC GTATCC(6/5)
BclI 1432 TGATCA T^GATCA
BfrI 3545 CTTAAG C^TTAAG
BsiEI 215 CGGCCG CG[GA][CT]^CG
BsmI 3865 GAATGC GAATGC(1/-1)
BspHI 1781 TCATGA T^CATGA
BspLU11I 3936 ACATGT A^CATGT
BsrDI 5027 GCAATG GCAATG(2/0)
BstBI 1867 TTCGAA TT^CGAA
BstXI 4862 CCAGATGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 215 CGGCCG C^GGCCG
Eam1105I 10 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 215 CGGCCG C^GGCCG
Eco52I 215 CGGCCG C^GGCCG
EcoRV 2322 GATATC GAT^ATC
FbaI 1432 TGATCA T^GATCA
FspI 2587 TGCGCA TGC^GCA
KspI 200 CCGCGG CCGC^GG
NspV 1867 TTCGAA TT^CGAA
PaeR7I 622 CTCGAG C^TCGAG
PflFI 4953 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 897 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 480 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4295 ATTAAT AT^TAAT
RcaI 1781 TCATGA T^CATGA
SacII 200 CCGCGG CCGC^GG
SbfI 648 CCTGCAGG CCTGCA^GG
SfiI 1821 GGCCATTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1867 TTCGAA TT^CGAA
SmaI 45 CCCGGG CCC^GGG
SpeI 516 ACTAGT A^CTAGT
Sse8387I 648 CCTGCAGG CCTGCA^GG
SspI 1212 AATATT AAT^ATT
StuI 4795 AGGCCT AGG^CCT
Tth111I 4953 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 897 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 413 CCACCCTCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 622 CTCGAG C^TCGAG
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
XmnI 147 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 238 GACGTC GACGT^C
2481 GACGTC
AcsI 2846 GAATTT [GA]^AATT[CT]
3886 GAATTT
AcyI 238 GACGTC G[GA]^CG[CT]C
2481 GACGTC
AflIII 2250 ACACGT A^C[GA][CT]GT
3936 ACATGT
ApoI 2846 GAATTT [GA]^AATT[CT]
3886 GAATTT
BsaHI 238 GACGTC G[GA]^CG[CT]C
2481 GACGTC
Bst1107I 338 GTATAC GTA^TAC
1989 GTATAC
BstAPI 126 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2699 GCAGCCTATGC
BstEII 892 GGTCACC G^GT[ACGT]ACC
2664 GGTGACC
BstPI 892 GGTCACC G^GT[ACGT]ACC
2664 GGTGACC
BstZ17I 338 GTATAC GTA^TAC
1989 GTATAC
EcoO65I 892 GGTCACC G^GT[ACGT]ACC
2664 GGTGACC
EcoT22I 634 ATGCAT ATGCA^T
3567 ATGCAT
HaeII 495 GGCGCT [GA]GCGC^[CT]
1722 AGCGCC
HgaI 598 GACGC GACGC(5/10)
2609 GCGTC
Hin1I 238 GACGTC G[GA]^CG[CT]C
2481 GACGTC
HincII 3932 GTTAAC GT[CT]^[GA]AC
4991 GTTAAC
HindII 3932 GTTAAC GT[CT]^[GA]AC
4991 GTTAAC
HindIII 445 AAGCTT A^AGCTT
1460 AAGCTT
HpaI 3932 GTTAAC GTT^AAC
4991 GTTAAC
NsiI 634 ATGCAT ATGCA^T
3567 ATGCAT
SacI 1389 GAGCTC GAGCT^C
4763 GAGCTC
SapI 863 GCTCTTC GCTCTTC(1/4)
3765 GCTCTTC
XbaI 1676 TCTAGA T^CTAGA
5177 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeI
NdeII NgoMIV NheI NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII