################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## Acc65I AccIII AclI AgeI Aor51HI AscI Asp718I AvrII BbeI BbrPI BlnI BsaAI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII BssSI ClaI CpoI DraIII DrdI Eco47III EcoRI FseI KasI KpnI MamI MfeI MluI MroI MunI NarI NotI NruI PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI ScaI SexAI SfoI SgrAI SnaBI SphI SspBI SwaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AflII 3545 CTTAAG C^TTAAG AhdI 10 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 520 GTGCAC G^TGCAC ApaI 42 GGGCCC GGGCC^C ApaLI 520 GTGCAC G^TGCAC AseI 4295 ATTAAT AT^TAAT AsnI 4295 ATTAAT AT^TAAT Asp700I 147 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 10 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4953 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2587 TGCGCA TGC^GCA BamHI 2497 GGATCC G^GATCC BanI 672 GGCACC G^G[CT][GA]CC BbvCI 1718 CCTCAGC CCTCAGC(-5/-2) BcgI 786 GCAAGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4376 GTATCC GTATCC(6/5) BclI 1432 TGATCA T^GATCA BfrI 3545 CTTAAG C^TTAAG BsiEI 215 CGGCCG CG[GA][CT]^CG BsmI 3865 GAATGC GAATGC(1/-1) BspHI 1781 TCATGA T^CATGA BspLU11I 3936 ACATGT A^CATGT BsrDI 5027 GCAATG GCAATG(2/0) BstBI 1867 TTCGAA TT^CGAA BstXI 4862 CCAGATGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 215 CGGCCG C^GGCCG Eam1105I 10 GACGGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 215 CGGCCG C^GGCCG Eco52I 215 CGGCCG C^GGCCG EcoRV 2322 GATATC GAT^ATC FbaI 1432 TGATCA T^GATCA FspI 2587 TGCGCA TGC^GCA KspI 200 CCGCGG CCGC^GG NspV 1867 TTCGAA TT^CGAA PaeR7I 622 CTCGAG C^TCGAG PflFI 4953 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 897 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 480 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4295 ATTAAT AT^TAAT RcaI 1781 TCATGA T^CATGA SacII 200 CCGCGG CCGC^GG SbfI 648 CCTGCAGG CCTGCA^GG SfiI 1821 GGCCATTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1867 TTCGAA TT^CGAA SmaI 45 CCCGGG CCC^GGG SpeI 516 ACTAGT A^CTAGT Sse8387I 648 CCTGCAGG CCTGCA^GG SspI 1212 AATATT AAT^ATT StuI 4795 AGGCCT AGG^CCT Tth111I 4953 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 897 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 413 CCACCCTCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 622 CTCGAG C^TCGAG XmaCI 45 CCCGGG C^CCGGG XmaI 45 CCCGGG C^CCGGG XmnI 147 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AatII 238 GACGTC GACGT^C 2481 GACGTC AcsI 2846 GAATTT [GA]^AATT[CT] 3886 GAATTT AcyI 238 GACGTC G[GA]^CG[CT]C 2481 GACGTC AflIII 2250 ACACGT A^C[GA][CT]GT 3936 ACATGT ApoI 2846 GAATTT [GA]^AATT[CT] 3886 GAATTT BsaHI 238 GACGTC G[GA]^CG[CT]C 2481 GACGTC Bst1107I 338 GTATAC GTA^TAC 1989 GTATAC BstAPI 126 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2699 GCAGCCTATGC BstEII 892 GGTCACC G^GT[ACGT]ACC 2664 GGTGACC BstPI 892 GGTCACC G^GT[ACGT]ACC 2664 GGTGACC BstZ17I 338 GTATAC GTA^TAC 1989 GTATAC EcoO65I 892 GGTCACC G^GT[ACGT]ACC 2664 GGTGACC EcoT22I 634 ATGCAT ATGCA^T 3567 ATGCAT HaeII 495 GGCGCT [GA]GCGC^[CT] 1722 AGCGCC HgaI 598 GACGC GACGC(5/10) 2609 GCGTC Hin1I 238 GACGTC G[GA]^CG[CT]C 2481 GACGTC HincII 3932 GTTAAC GT[CT]^[GA]AC 4991 GTTAAC HindII 3932 GTTAAC GT[CT]^[GA]AC 4991 GTTAAC HindIII 445 AAGCTT A^AGCTT 1460 AAGCTT HpaI 3932 GTTAAC GTT^AAC 4991 GTTAAC NsiI 634 ATGCAT ATGCA^T 3567 ATGCAT SacI 1389 GAGCTC GAGCT^C 4763 GAGCTC SapI 863 GCTCTTC GCTCTTC(1/4) 3765 GCTCTTC XbaI 1676 TCTAGA T^CTAGA 5177 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII