################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AccI AccIII AclI AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvrII BbeI BbvCI BclI BglII BlnI BsaI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstZ17I ClaI DrdI Eam1105I Eco47III FbaI FseI HpaI KasI KpnI MfeI MluI MroI MunI NarI NotI NruI PacI PflFI PinAI PmeI PshBI Psp1406I PvuI SalI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 364 GACGTC GACGT^C AccII 3457 CGCG CG^CG Alw44I 2901 GTGCAC G^TGCAC ApaI 3011 GGGCCC GGGCC^C ApaLI 2901 GTGCAC G^TGCAC Asp700I 2359 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 1364 CACGTG CAC^GTG BsaAI 1364 CACGTG [CT]AC^GT[GA] BsaBI 866 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 3023 CGGCCG CG[GA][CT]^CG BsmBI 902 GAGACG CGTCTC(1/5) BspLU11I 4045 ACATGT A^CATGT BsrDI 1218 GCAATG GCAATG(2/0) BssSI 2525 CTCGTG CACGAG(-5/-1) BstAPI 1360 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 781 GGTTACC G^GT[ACGT]ACC BstPI 781 GGTTACC G^GT[ACGT]ACC BstUI 3457 CGCG CG^CG CpoI 1914 CGGACCG CG^G[AT]CCG DraIII 1169 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 3023 CGGCCG C^GGCCG EclXI 3023 CGGCCG C^GGCCG Eco52I 3023 CGGCCG C^GGCCG EcoO65I 781 GGTTACC G^GT[ACGT]ACC EcoRI 751 GAATTC G^AATTC EcoT22I 281 ATGCAT ATGCA^T HincII 1744 GTCAAC GT[CT]^[GA]AC HindII 1744 GTCAAC GT[CT]^[GA]AC HindIII 923 AAGCTT A^AGCTT KspI 3456 CCGCGG CCGC^GG MamI 866 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 3839 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 3457 CGCG CG^CG NdeI 2966 CATATG CA^TATG NheI 1801 GCTAGC G^CTAGC NsiI 281 ATGCAT ATGCA^T PmaCI 1364 CACGTG CAC^GTG PmlI 1364 CACGTG CAC^GTG RsrII 1914 CGGACCG CG^G[AT]CCG SacII 3456 CCGCGG CCGC^GG SapI 3341 GAAGAGC GCTCTTC(1/4) StuI 2082 AGGCCT AGG^CCT XmnI 2359 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AflII 827 CTTAAG C^TTAAG 920 CTTAAG AflIII 1880 ACACGT A^C[GA][CT]GT 4045 ACATGT AocI 2078 CCTCAGG CC^T[ACGT]AGG 3356 CCTCAGG AviII 65 TGCGCA TGC^GCA 2970 TGCGCA BalI 306 TGGCCA TGG^CCA 4093 TGGCCA BcgI 1434 CGAAATCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3523 CGAGGATTCTGC BciVI 977 GTATCC GTATCC(6/5) 3935 GTATCC BfrI 827 CTTAAG C^TTAAG 920 CTTAAG BglI 2796 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3124 GCCCAGGAGGC BspMI 1824 ACCTGC ACCTGC(4/8) 3851 ACCTGC BsrBI 3175 CCGCTC CCGCTC(-3/-3) 1691 GAGCGG BstBI 31 TTCGAA TT^CGAA 3916 TTCGAA Bsu36I 2078 CCTCAGG CC^T[ACGT]AGG 3356 CCTCAGG DraI 836 TTTAAA TTT^AAA 1232 TTTAAA Eco81I 2078 CCTCAGG CC^T[ACGT]AGG 3356 CCTCAGG EcoRV 866 GATATC GAT^ATC 1394 GATATC FspI 65 TGCGCA TGC^GCA 2970 TGCGCA HaeII 108 AGCGCC [GA]GCGC^[CT] 3251 AGCGCC MluNI 306 TGGCCA TGG^CCA 4093 TGGCCA MscI 306 TGGCCA TGG^CCA 4093 TGGCCA NaeI 120 GCCGGC GCC^GGC 1214 GCCGGC NcoI 1053 CCATGG C^CATGG 2053 CCATGG NgoMIV 120 GCCGGC G^CCGGC 1214 GCCGGC NspV 31 TTCGAA TT^CGAA 3916 TTCGAA PaeR7I 3178 CTCGAG C^TCGAG 3375 CTCGAG PshAI 2363 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3732 GACGCCTGTC SacI 2401 GAGCTC GAGCT^C 3094 GAGCTC ScaI 275 AGTACT AGT^ACT 3952 AGTACT SfuI 31 TTCGAA TT^CGAA 3916 TTCGAA SmaI 50 CCCGGG CCC^GGG 771 CCCGGG SspI 1162 AATATT AAT^ATT 4127 AATATT XhoI 3178 CTCGAG C^TCGAG 3375 CTCGAG XmaCI 50 CCCGGG C^CCGGG 771 CCCGGG XmaI 50 CCCGGG C^CCGGG 771 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII