##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbeI
BbvCI BclI BglII BlnI BsaI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstZ17I
ClaI DrdI Eam1105I Eco47III FbaI
FseI HpaI KasI KpnI MfeI
MluI MroI MunI NarI NotI
NruI PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI SalI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 364 GACGTC GACGT^C
AccII 3457 CGCG CG^CG
Alw44I 2901 GTGCAC G^TGCAC
ApaI 3011 GGGCCC GGGCC^C
ApaLI 2901 GTGCAC G^TGCAC
Asp700I 2359 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1364 CACGTG CAC^GTG
BsaAI 1364 CACGTG [CT]AC^GT[GA]
BsaBI 866 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3023 CGGCCG CG[GA][CT]^CG
BsmBI 902 GAGACG CGTCTC(1/5)
BspLU11I 4045 ACATGT A^CATGT
BsrDI 1218 GCAATG GCAATG(2/0)
BssSI 2525 CTCGTG CACGAG(-5/-1)
BstAPI 1360 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 781 GGTTACC G^GT[ACGT]ACC
BstPI 781 GGTTACC G^GT[ACGT]ACC
BstUI 3457 CGCG CG^CG
CpoI 1914 CGGACCG CG^G[AT]CCG
DraIII 1169 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3023 CGGCCG C^GGCCG
EclXI 3023 CGGCCG C^GGCCG
Eco52I 3023 CGGCCG C^GGCCG
EcoO65I 781 GGTTACC G^GT[ACGT]ACC
EcoRI 751 GAATTC G^AATTC
EcoT22I 281 ATGCAT ATGCA^T
HincII 1744 GTCAAC GT[CT]^[GA]AC
HindII 1744 GTCAAC GT[CT]^[GA]AC
HindIII 923 AAGCTT A^AGCTT
KspI 3456 CCGCGG CCGC^GG
MamI 866 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 3839 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 3457 CGCG CG^CG
NdeI 2966 CATATG CA^TATG
NheI 1801 GCTAGC G^CTAGC
NsiI 281 ATGCAT ATGCA^T
PmaCI 1364 CACGTG CAC^GTG
PmlI 1364 CACGTG CAC^GTG
RsrII 1914 CGGACCG CG^G[AT]CCG
SacII 3456 CCGCGG CCGC^GG
SapI 3341 GAAGAGC GCTCTTC(1/4)
StuI 2082 AGGCCT AGG^CCT
XmnI 2359 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflII 827 CTTAAG C^TTAAG
920 CTTAAG
AflIII 1880 ACACGT A^C[GA][CT]GT
4045 ACATGT
AocI 2078 CCTCAGG CC^T[ACGT]AGG
3356 CCTCAGG
AviII 65 TGCGCA TGC^GCA
2970 TGCGCA
BalI 306 TGGCCA TGG^CCA
4093 TGGCCA
BcgI 1434 CGAAATCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3523 CGAGGATTCTGC
BciVI 977 GTATCC GTATCC(6/5)
3935 GTATCC
BfrI 827 CTTAAG C^TTAAG
920 CTTAAG
BglI 2796 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3124 GCCCAGGAGGC
BspMI 1824 ACCTGC ACCTGC(4/8)
3851 ACCTGC
BsrBI 3175 CCGCTC CCGCTC(-3/-3)
1691 GAGCGG
BstBI 31 TTCGAA TT^CGAA
3916 TTCGAA
Bsu36I 2078 CCTCAGG CC^T[ACGT]AGG
3356 CCTCAGG
DraI 836 TTTAAA TTT^AAA
1232 TTTAAA
Eco81I 2078 CCTCAGG CC^T[ACGT]AGG
3356 CCTCAGG
EcoRV 866 GATATC GAT^ATC
1394 GATATC
FspI 65 TGCGCA TGC^GCA
2970 TGCGCA
HaeII 108 AGCGCC [GA]GCGC^[CT]
3251 AGCGCC
MluNI 306 TGGCCA TGG^CCA
4093 TGGCCA
MscI 306 TGGCCA TGG^CCA
4093 TGGCCA
NaeI 120 GCCGGC GCC^GGC
1214 GCCGGC
NcoI 1053 CCATGG C^CATGG
2053 CCATGG
NgoMIV 120 GCCGGC G^CCGGC
1214 GCCGGC
NspV 31 TTCGAA TT^CGAA
3916 TTCGAA
PaeR7I 3178 CTCGAG C^TCGAG
3375 CTCGAG
PshAI 2363 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3732 GACGCCTGTC
SacI 2401 GAGCTC GAGCT^C
3094 GAGCTC
ScaI 275 AGTACT AGT^ACT
3952 AGTACT
SfuI 31 TTCGAA TT^CGAA
3916 TTCGAA
SmaI 50 CCCGGG CCC^GGG
771 CCCGGG
SspI 1162 AATATT AAT^ATT
4127 AATATT
XhoI 3178 CTCGAG C^TCGAG
3375 CTCGAG
XmaCI 50 CCCGGG C^CCGGG
771 CCCGGG
XmaI 50 CCCGGG C^CCGGG
771 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII