################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII AccIII AclI AcsI AgeI AhdI ApoI AscI Asp700I AspEI AspI AviII AvrII BbeI BclI BlnI BsaBI BsaWI BseAI BsiEI BsmBI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRI FbaI FseI FspI KasI MamI MfeI MluI MroI MunI NarI NotI NruI NspV PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 4815 GGTACC G^GTACC AflII 634 CTTAAG C^TTAAG Aor51HI 2501 AGCGCT AGC^GCT AseI 1169 ATTAAT AT^TAAT AsnI 1169 ATTAAT AT^TAAT Asp718I 4815 GGTACC G^GTACC BbrPI 2904 CACGTG CAC^GTG BcgI 3960 CGATGCATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3663 GTATCC GTATCC(6/5) BfrI 634 CTTAAG C^TTAAG BglI 2052 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2904 CACGTG [CT]AC^GT[GA] BsiWI 4265 CGTACG C^GTACG BsmI 3805 GAATGC GAATGC(1/-1) Bsp1407I 2477 TGTACA T^GTACA BspHI 754 TCATGA T^CATGA BsrFI 4618 GCCGGC [GA]^CCGG[CT] BsrGI 2477 TGTACA T^GTACA BstEII 639 GGTAACC G^GT[ACGT]ACC BstPI 639 GGTAACC G^GT[ACGT]ACC Cfr10I 4618 GCCGGC [GA]^CCGG[CT] DraIII 4305 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4477 GACAATAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2501 AGCGCT AGC^GCT EcoO65I 639 GGTAACC G^GT[ACGT]ACC EcoRV 3257 GATATC GAT^ATC HpaI 680 GTTAAC GTT^AAC KpnI 4815 GGTACC GGTAC^C KspI 4754 CCGCGG CCGC^GG NaeI 4618 GCCGGC GCC^GGC NgoMIV 4618 GCCGGC G^CCGGC NheI 1191 GCTAGC G^CTAGC PacI 1166 TTAATTAA TTAAT^TAA PaeR7I 4415 CTCGAG C^TCGAG PmaCI 2904 CACGTG CAC^GTG PmlI 2904 CACGTG CAC^GTG PshBI 1169 ATTAAT AT^TAAT RcaI 754 TCATGA T^CATGA SacI 252 GAGCTC GAGCT^C SacII 4754 CCGCGG CCGC^GG SmaI 4277 CCCGGG CCC^GGG SspBI 2477 TGTACA T^GTACA StuI 4892 AGGCCT AGG^CCT XbaI 2489 TCTAGA T^CTAGA XhoI 4415 CTCGAG C^TCGAG XmaCI 4277 CCCGGG C^CCGGG XmaI 4277 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AocI 838 CCTCAGG CC^T[ACGT]AGG 1624 CCTAAGG ApaI 1676 GGGCCC GGGCC^C 1967 GGGCCC BamHI 813 GGATCC G^GATCC 1482 GGATCC BbsI 714 GTCTTC GAAGAC(2/6) 1378 GTCTTC BglII 2358 AGATCT A^GATCT 2448 AGATCT BpuAI 714 GTCTTC GAAGAC(2/6) 1378 GTCTTC BsrDI 3548 CATTGC GCAATG(2/0) 3828 CATTGC Bsu36I 838 CCTCAGG CC^T[ACGT]AGG 1624 CCTAAGG DraI 2735 TTTAAA TTT^AAA 3005 TTTAAA Eco81I 838 CCTCAGG CC^T[ACGT]AGG 1624 CCTAAGG PleI 3366 GACTC GAGTC(4/5) 2889 GAGTC SalI 1931 GTCGAC G^TCGAC 4313 GTCGAC SphI 1232 GCATGC GCATG^C 4289 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII