##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AcsI AgeI
AhdI ApoI AscI Asp700I AspEI
AspI AviII AvrII BbeI BclI
BlnI BsaBI BsaWI BseAI BsiEI
BsmBI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRI
FbaI FseI FspI KasI MamI
MfeI MluI MroI MunI NarI
NotI NruI NspV PflFI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 4815 GGTACC G^GTACC
AflII 634 CTTAAG C^TTAAG
Aor51HI 2501 AGCGCT AGC^GCT
AseI 1169 ATTAAT AT^TAAT
AsnI 1169 ATTAAT AT^TAAT
Asp718I 4815 GGTACC G^GTACC
BbrPI 2904 CACGTG CAC^GTG
BcgI 3960 CGATGCATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3663 GTATCC GTATCC(6/5)
BfrI 634 CTTAAG C^TTAAG
BglI 2052 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2904 CACGTG [CT]AC^GT[GA]
BsiWI 4265 CGTACG C^GTACG
BsmI 3805 GAATGC GAATGC(1/-1)
Bsp1407I 2477 TGTACA T^GTACA
BspHI 754 TCATGA T^CATGA
BsrFI 4618 GCCGGC [GA]^CCGG[CT]
BsrGI 2477 TGTACA T^GTACA
BstEII 639 GGTAACC G^GT[ACGT]ACC
BstPI 639 GGTAACC G^GT[ACGT]ACC
Cfr10I 4618 GCCGGC [GA]^CCGG[CT]
DraIII 4305 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4477 GACAATAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2501 AGCGCT AGC^GCT
EcoO65I 639 GGTAACC G^GT[ACGT]ACC
EcoRV 3257 GATATC GAT^ATC
HpaI 680 GTTAAC GTT^AAC
KpnI 4815 GGTACC GGTAC^C
KspI 4754 CCGCGG CCGC^GG
NaeI 4618 GCCGGC GCC^GGC
NgoMIV 4618 GCCGGC G^CCGGC
NheI 1191 GCTAGC G^CTAGC
PacI 1166 TTAATTAA TTAAT^TAA
PaeR7I 4415 CTCGAG C^TCGAG
PmaCI 2904 CACGTG CAC^GTG
PmlI 2904 CACGTG CAC^GTG
PshBI 1169 ATTAAT AT^TAAT
RcaI 754 TCATGA T^CATGA
SacI 252 GAGCTC GAGCT^C
SacII 4754 CCGCGG CCGC^GG
SmaI 4277 CCCGGG CCC^GGG
SspBI 2477 TGTACA T^GTACA
StuI 4892 AGGCCT AGG^CCT
XbaI 2489 TCTAGA T^CTAGA
XhoI 4415 CTCGAG C^TCGAG
XmaCI 4277 CCCGGG C^CCGGG
XmaI 4277 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AocI 838 CCTCAGG CC^T[ACGT]AGG
1624 CCTAAGG
ApaI 1676 GGGCCC GGGCC^C
1967 GGGCCC
BamHI 813 GGATCC G^GATCC
1482 GGATCC
BbsI 714 GTCTTC GAAGAC(2/6)
1378 GTCTTC
BglII 2358 AGATCT A^GATCT
2448 AGATCT
BpuAI 714 GTCTTC GAAGAC(2/6)
1378 GTCTTC
BsrDI 3548 CATTGC GCAATG(2/0)
3828 CATTGC
Bsu36I 838 CCTCAGG CC^T[ACGT]AGG
1624 CCTAAGG
DraI 2735 TTTAAA TTT^AAA
3005 TTTAAA
Eco81I 838 CCTCAGG CC^T[ACGT]AGG
1624 CCTAAGG
PleI 3366 GACTC GAGTC(4/5)
2889 GAGTC
SalI 1931 GTCGAC G^TCGAC
4313 GTCGAC
SphI 1232 GCATGC GCATG^C
4289 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PflMI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII