##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
ApoI AscI AseI AsnI AviII
BbeI BbrPI BciVI BclI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BssHII BstBI ClaI CpoI EagI
EclXI Eco52I EcoRI EcoT22I FbaI
FseI FspI Hin1I HpaI KasI
KspI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PmaCI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SexAI SfoI SfuI
SgrAI SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 109 CGCG CG^CG
AflII 1049 CTTAAG C^TTAAG
AflIII 2157 ACATGT A^C[GA][CT]GT
AgeI 2106 ACCGGT A^CCGGT
AhdI 3660 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 700 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3660 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4262 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1810 GGATCC G^GATCC
BbvCI 3468 GCTGAGG CCTCAGC(-5/-2)
BcgI 501 CGACTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1049 CTTAAG C^TTAAG
BglI 1933 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1805 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2106 ACCGGT [AT]^CCGG[AT]
BspLU11I 2157 ACATGT A^CATGT
BsrDI 2372 GCAATG GCAATG(2/0)
Bst1107I 2237 GTATAC GTA^TAC
BstEII 3599 GGTGACC G^GT[ACGT]ACC
BstPI 3599 GGTGACC G^GT[ACGT]ACC
BstUI 109 CGCG CG^CG
BstXI 1270 CCAGGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2237 GTATAC GTA^TAC
DraI 566 TTTAAA TTT^AAA
Eam1105I 3660 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3599 GGTGACC G^GT[ACGT]ACC
EcoRV 1885 GATATC GAT^ATC
FauI 726 GCGGG CCCGC(4/6)
HgaI 3362 GCGTC GACGC(5/10)
HincII 1440 GTTGAC GT[CT]^[GA]AC
HindII 1440 GTTGAC GT[CT]^[GA]AC
HindIII 3907 AAGCTT A^AGCTT
MamI 1805 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 2154 CACACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 109 CGCG CG^CG
PaeR7I 3727 CTCGAG C^TCGAG
PflFI 4262 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2106 ACCGGT A^CCGGT
PmeI 565 GTTTAAAC GTTT^AAAC
PshAI 1784 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 889 GAAGAGC GCTCTTC(1/4)
SbfI 2793 CCTGCAGG CCTGCA^GG
ScaI 1611 AGTACT AGT^ACT
SfiI 1932 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1839 CCCGGG CCC^GGG
SpeI 2557 ACTAGT A^CTAGT
SphI 3770 GCATGC GCATG^C
Sse8387I 2793 CCTGCAGG CCTGCA^GG
SspI 4006 AATATT AAT^ATT
Tsp509I 3542 AATT ^AATT
Tth111I 4262 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2336 TCTAGA T^CTAGA
XhoI 3727 CTCGAG C^TCGAG
XmaCI 1839 CCCGGG C^CCGGG
XmaI 1839 CCCGGG C^CCGGG
XmnI 700 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2237 GTATAC GT^[AC][GT]AC
2426 GTCTAC
Aor51HI 1242 AGCGCT AGC^GCT
1456 AGCGCT
AvrII 1536 CCTAGG C^CTAGG
3655 CCTAGG
BalI 2536 TGGCCA TGG^CCA
3289 TGGCCA
BbsI 3438 GAAGAC GAAGAC(2/6)
525 GTCTTC
BglII 753 AGATCT A^GATCT
4360 AGATCT
BlnI 1536 CCTAGG C^CTAGG
3655 CCTAGG
BlpI 388 GCTAAGC GC^T[ACGT]AGC
2115 GCTCAGC
Bpu1102I 388 GCTAAGC GC^T[ACGT]AGC
2115 GCTCAGC
BpuAI 3438 GAAGAC GAAGAC(2/6)
525 GTCTTC
BstAPI 1326 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3904 GCAAAGCTTGC
CelII 388 GCTAAGC GC^T[ACGT]AGC
2115 GCTCAGC
DraIII 785 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1594 CACTCTGTG
Eco47III 1242 AGCGCT AGC^GCT
1456 AGCGCT
MluNI 2536 TGGCCA TGG^CCA
3289 TGGCCA
MscI 2536 TGGCCA TGG^CCA
3289 TGGCCA
PflMI 952 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3894 CCAGGTTCTGG
SacI 1686 GAGCTC GAGCT^C
3550 GAGCTC
Van91I 952 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3894 CCAGGTTCTGG
XcmI 2263 CCAAATGGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4220 CCAGATGTTCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
Acc65I AciI AfaI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
Asp718I AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrFI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I XhoII