##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AclI AflII AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BcgI
BciVI BfrI BglI BglII BlnI
BsaBI BsaI BsiEI BsmBI BspDI
BspHI BspLU11I BspMI BssHII BssSI
Bst1107I BstZ17I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco52I FseI
FspI HindIII HpaI KpnI KspI
MamI NaeI NdeI NgoMIV NheI
NotI PacI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SfiI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1135 GACGTC GACGT^C
AccI 1195 GTCTAC GT^[AC][GT]AC
AccIII 1601 TCCGGA T^CCGGA
AocI 1746 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2620 AGCGCT AGC^GCT
Asp700I 2329 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 2773 GGCGCC GGCGC^C
BbrPI 2428 CACGTG CAC^GTG
BbsI 976 GTCTTC GAAGAC(2/6)
BlpI 2794 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2794 GCTGAGC GC^T[ACGT]AGC
BpuAI 976 GTCTTC GAAGAC(2/6)
BseAI 1601 TCCGGA T^CCGGA
BsiWI 322 CGTACG C^GTACG
BspEI 1601 TCCGGA T^CCGGA
BsrBI 2409 CCGCTC CCGCTC(-3/-3)
BstAPI 1926 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1746 CCTAAGG CC^T[ACGT]AGG
CelII 2794 GCTGAGC GC^T[ACGT]AGC
DraI 3271 TTTAAA TTT^AAA
DrdI 967 GACATGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2620 AGCGCT AGC^GCT
Eco81I 1746 CCTAAGG CC^T[ACGT]AGG
EcoNI 1071 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1060 GAATTC G^AATTC
EcoRV 2896 GATATC GAT^ATC
EcoT22I 2827 ATGCAT ATGCA^T
KasI 2773 GGCGCC G^GCGCC
MfeI 1611 CAATTG C^AATTG
MluI 325 ACGCGT A^CGCGT
MroI 1601 TCCGGA T^CCGGA
MunI 1611 CAATTG C^AATTG
NarI 2773 GGCGCC GG^CGCC
NsiI 2827 ATGCAT ATGCA^T
NspI 968 ACATGC [GA]CATG^[CT]
PaeR7I 746 CTCGAG C^TCGAG
PmaCI 2428 CACGTG CAC^GTG
PmlI 2428 CACGTG CAC^GTG
PshAI 913 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2968 CAGCTG CAG^CTG
ScaI 1769 AGTACT AGT^ACT
SexAI 1325 ACCAGGT A^CC[AT]GGT
SfoI 2773 GGCGCC GGC^GCC
SgrAI 372 CACCGGTG C[GA]^CCGG[CT]G
SmaI 561 CCCGGG CCC^GGG
StuI 3142 AGGCCT AGG^CCT
XhoI 746 CTCGAG C^TCGAG
XmaCI 561 CCCGGG C^CCGGG
XmaI 561 CCCGGG C^CCGGG
XmnI 2329 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 1135 GACGTC G[GA]^CG[CT]C
2773 GGCGCC
AflIII 325 ACGCGT A^C[GA][CT]GT
618 ACGTGT
AgeI 373 ACCGGT A^CCGGT
1152 ACCGGT
AlwNI 893 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1927 CAGACACTG
ApaI 436 GGGCCC GGGCC^C
2040 GGGCCC
AspI 69 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1324 GACCAGGTC
BclI 1175 TGATCA T^GATCA
2060 TGATCA
BpmI 2738 CTCCAG CTGGAG(16/14)
2752 CTGGAG
BsaAI 2116 TACGTG [CT]AC^GT[GA]
2428 CACGTG
BsaHI 1135 GACGTC G[GA]^CG[CT]C
2773 GGCGCC
BsgI 452 CTGCAC GTGCAG(16/14)
622 GTGCAG
BsmI 2350 GAATGC GAATGC(1/-1)
1541 GCATTC
Bsp1407I 855 TGTACA T^GTACA
3134 TGTACA
BsrFI 373 ACCGGT [GA]^CCGG[CT]
1152 ACCGGT
BsrGI 855 TGTACA T^GTACA
3134 TGTACA
BstBI 162 TTCGAA TT^CGAA
1315 TTCGAA
BstYI 1598 AGATCC [GA]^GATC[CT]
2299 GGATCT
Cfr10I 373 ACCGGT [GA]^CCGG[CT]
1152 ACCGGT
FbaI 1175 TGATCA T^GATCA
2060 TGATCA
Hin1I 1135 GACGTC G[GA]^CG[CT]C
2773 GGCGCC
HincII 2614 GTCAAC GT[CT]^[GA]AC
2836 GTCAAC
HindII 2614 GTCAAC GT[CT]^[GA]AC
2836 GTCAAC
MflI 1598 AGATCC [GA]^GATC[CT]
2299 GGATCT
NcoI 1410 CCATGG C^CATGG
2294 CCATGG
NruI 597 TCGCGA TCG^CGA
2516 TCGCGA
NspV 162 TTCGAA TT^CGAA
1315 TTCGAA
PflFI 69 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1324 GACCAGGTC
PflMI 178 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2991 CCAATGGTTGG
PinAI 373 ACCGGT A^CCGGT
1152 ACCGGT
PpuMI 1067 AGGTCCT [GA]G^G[AT]CC[CT]
2162 AGGACCT
SapI 51 GAAGAGC GCTCTTC(1/4)
2685 GAAGAGC
SfuI 162 TTCGAA TT^CGAA
1315 TTCGAA
SspBI 855 TGTACA T^GTACA
3134 TGTACA
Tth111I 69 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1324 GACCAGGTC
Van91I 178 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2991 CCAATGGTTGG
XhoII 1598 AGATCC [GA]^GATC[CT]
2299 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BmyI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI