################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## Acc65I AclI AflII AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BcgI BciVI BfrI BglI BglII BlnI BsaBI BsaI BsiEI BsmBI BspDI BspHI BspLU11I BspMI BssHII BssSI Bst1107I BstZ17I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I FseI FspI HindIII HpaI KpnI KspI MamI NaeI NdeI NgoMIV NheI NotI PacI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SfiI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 1135 GACGTC GACGT^C AccI 1195 GTCTAC GT^[AC][GT]AC AccIII 1601 TCCGGA T^CCGGA AocI 1746 CCTAAGG CC^T[ACGT]AGG Aor51HI 2620 AGCGCT AGC^GCT Asp700I 2329 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 2773 GGCGCC GGCGC^C BbrPI 2428 CACGTG CAC^GTG BbsI 976 GTCTTC GAAGAC(2/6) BlpI 2794 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2794 GCTGAGC GC^T[ACGT]AGC BpuAI 976 GTCTTC GAAGAC(2/6) BseAI 1601 TCCGGA T^CCGGA BsiWI 322 CGTACG C^GTACG BspEI 1601 TCCGGA T^CCGGA BsrBI 2409 CCGCTC CCGCTC(-3/-3) BstAPI 1926 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1746 CCTAAGG CC^T[ACGT]AGG CelII 2794 GCTGAGC GC^T[ACGT]AGC DraI 3271 TTTAAA TTT^AAA DrdI 967 GACATGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2620 AGCGCT AGC^GCT Eco81I 1746 CCTAAGG CC^T[ACGT]AGG EcoNI 1071 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1060 GAATTC G^AATTC EcoRV 2896 GATATC GAT^ATC EcoT22I 2827 ATGCAT ATGCA^T KasI 2773 GGCGCC G^GCGCC MfeI 1611 CAATTG C^AATTG MluI 325 ACGCGT A^CGCGT MroI 1601 TCCGGA T^CCGGA MunI 1611 CAATTG C^AATTG NarI 2773 GGCGCC GG^CGCC NsiI 2827 ATGCAT ATGCA^T NspI 968 ACATGC [GA]CATG^[CT] PaeR7I 746 CTCGAG C^TCGAG PmaCI 2428 CACGTG CAC^GTG PmlI 2428 CACGTG CAC^GTG PshAI 913 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 2968 CAGCTG CAG^CTG ScaI 1769 AGTACT AGT^ACT SexAI 1325 ACCAGGT A^CC[AT]GGT SfoI 2773 GGCGCC GGC^GCC SgrAI 372 CACCGGTG C[GA]^CCGG[CT]G SmaI 561 CCCGGG CCC^GGG StuI 3142 AGGCCT AGG^CCT XhoI 746 CTCGAG C^TCGAG XmaCI 561 CCCGGG C^CCGGG XmaI 561 CCCGGG C^CCGGG XmnI 2329 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcyI 1135 GACGTC G[GA]^CG[CT]C 2773 GGCGCC AflIII 325 ACGCGT A^C[GA][CT]GT 618 ACGTGT AgeI 373 ACCGGT A^CCGGT 1152 ACCGGT AlwNI 893 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG 1927 CAGACACTG ApaI 436 GGGCCC GGGCC^C 2040 GGGCCC AspI 69 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1324 GACCAGGTC BclI 1175 TGATCA T^GATCA 2060 TGATCA BpmI 2738 CTCCAG CTGGAG(16/14) 2752 CTGGAG BsaAI 2116 TACGTG [CT]AC^GT[GA] 2428 CACGTG BsaHI 1135 GACGTC G[GA]^CG[CT]C 2773 GGCGCC BsgI 452 CTGCAC GTGCAG(16/14) 622 GTGCAG BsmI 2350 GAATGC GAATGC(1/-1) 1541 GCATTC Bsp1407I 855 TGTACA T^GTACA 3134 TGTACA BsrFI 373 ACCGGT [GA]^CCGG[CT] 1152 ACCGGT BsrGI 855 TGTACA T^GTACA 3134 TGTACA BstBI 162 TTCGAA TT^CGAA 1315 TTCGAA BstYI 1598 AGATCC [GA]^GATC[CT] 2299 GGATCT Cfr10I 373 ACCGGT [GA]^CCGG[CT] 1152 ACCGGT FbaI 1175 TGATCA T^GATCA 2060 TGATCA Hin1I 1135 GACGTC G[GA]^CG[CT]C 2773 GGCGCC HincII 2614 GTCAAC GT[CT]^[GA]AC 2836 GTCAAC HindII 2614 GTCAAC GT[CT]^[GA]AC 2836 GTCAAC MflI 1598 AGATCC [GA]^GATC[CT] 2299 GGATCT NcoI 1410 CCATGG C^CATGG 2294 CCATGG NruI 597 TCGCGA TCG^CGA 2516 TCGCGA NspV 162 TTCGAA TT^CGAA 1315 TTCGAA PflFI 69 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1324 GACCAGGTC PflMI 178 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2991 CCAATGGTTGG PinAI 373 ACCGGT A^CCGGT 1152 ACCGGT PpuMI 1067 AGGTCCT [GA]G^G[AT]CC[CT] 2162 AGGACCT SapI 51 GAAGAGC GCTCTTC(1/4) 2685 GAAGAGC SfuI 162 TTCGAA TT^CGAA 1315 TTCGAA SspBI 855 TGTACA T^GTACA 3134 TGTACA Tth111I 69 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1324 GACCAGGTC Van91I 178 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2991 CCAATGGTTGG XhoII 1598 AGATCC [GA]^GATC[CT] 2299 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BmyI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI