##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AclI AgeI AhdI ApaI
AscI Asp718I AspEI AspI BcgI
BclI BglII BsaBI BsiWI BsmBI
BspHI BspLU11I BstBI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3076 GACGTC GACGT^C
AccI 1869 GTATAC GT^[AC][GT]AC
AccIII 327 TCCGGA T^CCGGA
AflII 4142 CTTAAG C^TTAAG
AocI 4163 CCTGAGG CC^T[ACGT]AGG
Asp700I 595 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1679 TGCGCA TGC^GCA
BamHI 2911 GGATCC G^GATCC
BbeI 2314 GGCGCC GGCGC^C
BbvCI 3443 CCTCAGC CCTCAGC(-5/-2)
BciVI 301 GGATAC GTATCC(6/5)
BfrI 4142 CTTAAG C^TTAAG
BseAI 327 TCCGGA T^CCGGA
BsiEI 1119 CGGTCG CG[GA][CT]^CG
BspDI 456 ATCGAT AT^CGAT
BspEI 327 TCCGGA T^CCGGA
BsrBI 502 CCGCTC CCGCTC(-3/-3)
BsrFI 2183 ACCGGC [GA]^CCGG[CT]
BssHII 1468 GCGCGC G^CGCGC
Bst1107I 1869 GTATAC GTA^TAC
BstAPI 3031 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1869 GTATAC GTA^TAC
Bsu36I 4163 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2183 ACCGGC [GA]^CCGG[CT]
ClaI 456 ATCGAT AT^CGAT
Eco81I 4163 CCTGAGG CC^T[ACGT]AGG
FspI 1679 TGCGCA TGC^GCA
HgaI 2302 GACGC GACGC(5/10)
KasI 2314 GGCGCC G^GCGCC
MroI 327 TCCGGA T^CCGGA
NarI 2314 GGCGCC GG^CGCC
PpuMI 3439 GGGACCT [GA]G^G[AT]CC[CT]
SfiI 1025 GGCCTCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2314 GGCGCC GGC^GCC
SspI 3364 AATATT AAT^ATT
XmnI 595 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 1038 ACGTGT A^C[GA][CT]GT
2270 ACACGT
Aor51HI 2045 AGCGCT AGC^GCT
2089 AGCGCT
AseI 2958 ATTAAT AT^TAAT
3186 ATTAAT
AsnI 2958 ATTAAT AT^TAAT
3186 ATTAAT
AvrII 2916 CCTAGG C^CTAGG
4227 CCTAGG
BlnI 2916 CCTAGG C^CTAGG
4227 CCTAGG
BseRI 1803 CTCCTC GAGGAG(10/8)
2031 CTCCTC
BssSI 1535 CTCGTG CACGAG(-5/-1)
2410 CTCGTG
BstEII 1596 GGTGACC G^GT[ACGT]ACC
2790 GGTCACC
BstPI 1596 GGTGACC G^GT[ACGT]ACC
2790 GGTCACC
BstYI 2911 GGATCC [GA]^GATC[CT]
3805 GGATCT
DraI 4285 TTTAAA TTT^AAA
4339 TTTAAA
DraII 2174 AGGGCCT [GA]G^G[ACGT]CC[CT]
3439 GGGACCT
DraIII 437 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2277 CACCCAGTG
Eco47III 2045 AGCGCT AGC^GCT
2089 AGCGCT
EcoO109I 2174 AGGGCCT [GA]G^G[ACGT]CC[CT]
3439 GGGACCT
EcoO65I 1596 GGTGACC G^GT[ACGT]ACC
2790 GGTCACC
MflI 2911 GGATCC [GA]^GATC[CT]
3805 GGATCT
PshAI 713 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3072 GACTGACGTC
PshBI 2958 ATTAAT AT^TAAT
3186 ATTAAT
PstI 2025 CTGCAG CTGCA^G
2337 CTGCAG
SapI 44 GAAGAGC GCTCTTC(1/4)
1353 GCTCTTC
XhoII 2911 GGATCC [GA]^GATC[CT]
3805 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CelII CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XmaCI XmaI