##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AgeI AocI ApoI
AscI AvrII BbeI BbrPI BbsI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BseAI BsiWI BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DrdI Eco81I EcoRI EcoRV
FseI Hin1I KasI KspI MamI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 673 GTAGAC GT^[AC][GT]AC
AhdI 3106 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 444 GTGCAC G^TGCAC
Aor51HI 4542 AGCGCT AGC^GCT
ApaLI 444 GTGCAC G^TGCAC
AseI 4463 ATTAAT AT^TAAT
AsnI 4463 ATTAAT AT^TAAT
AspEI 3106 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3622 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2294 TGCGCA TGC^GCA
BamHI 3368 GGATCC G^GATCC
BcgI 865 CGAGGAACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3459 GTATCC GTATCC(6/5)
BclI 4442 TGATCA T^GATCA
BglI 163 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 2623 TCCGGT [AT]^CCGG[AT]
BsiEI 103 CGGCCG CG[GA][CT]^CG
BsmBI 1170 GAGACG CGTCTC(1/5)
BsmI 4167 GAATGC GAATGC(1/-1)
BstYI 3368 GGATCC [GA]^GATC[CT]
DraI 1810 TTTAAA TTT^AAA
EagI 103 CGGCCG C^GGCCG
Eam1105I 3106 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 103 CGGCCG C^GGCCG
Eco47III 4542 AGCGCT AGC^GCT
Eco52I 103 CGGCCG C^GGCCG
FbaI 4442 TGATCA T^GATCA
FspI 2294 TGCGCA TGC^GCA
HindIII 4263 AAGCTT A^AGCTT
HpaI 3337 GTTAAC GTT^AAC
MfeI 785 CAATTG C^AATTG
MflI 3368 GGATCC [GA]^GATC[CT]
MunI 785 CAATTG C^AATTG
PaeR7I 863 CTCGAG C^TCGAG
PflFI 3622 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1300 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4463 ATTAAT AT^TAAT
SacI 2117 GAGCTC GAGCT^C
SbfI 732 CCTGCAGG CCTGCA^GG
ScaI 1832 AGTACT AGT^ACT
SmaI 2760 CCCGGG CCC^GGG
SpeI 1678 ACTAGT A^CTAGT
Sse8387I 732 CCTGCAGG CCTGCA^GG
Tth111I 3622 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3425 TCTAGA T^CTAGA
XhoI 863 CTCGAG C^TCGAG
XhoII 3368 GGATCC [GA]^GATC[CT]
XmaCI 2760 CCCGGG C^CCGGG
XmaI 2760 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1864 GGTACC G^GTACC
3824 GGTACC
Asp700I 2587 GAATGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4517 GAACCTTTTC
Asp718I 1864 GGTACC G^GTACC
3824 GGTACC
AvaI 863 CTCGAG C^[CT]CG[GA]G
2760 CCCGGG
BbvCI 3537 CCTCAGC CCTCAGC(-5/-2)
1458 GCTGAGG
BcnI 2760 CCCGG CC^[GC]GG
2761 CCGGG
BsoBI 863 CTCGAG C^[CT]CG[GA]G
2760 CCCGGG
BstAPI 1205 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2664 GCAAGTCTTGC
BstEII 1017 GGTCACC G^GT[ACGT]ACC
3128 GGTAACC
BstPI 1017 GGTCACC G^GT[ACGT]ACC
3128 GGTAACC
DsaI 1293 CCATGG C^C[GA][CT]GG
4116 CCATGG
EcoO65I 1017 GGTCACC G^GT[ACGT]ACC
3128 GGTAACC
EcoT22I 1377 ATGCAT ATGCA^T
3604 ATGCAT
HaeII 994 AGCGCC [GA]GCGC^[CT]
4542 AGCGCT
HapII 2624 CCGG C^CGG
2761 CCGG
HgaI 147 GACGC GACGC(5/10)
4101 GACGC
HincII 3161 GTTGAC GT[CT]^[GA]AC
3337 GTTAAC
HindII 3161 GTTGAC GT[CT]^[GA]AC
3337 GTTAAC
HpaII 2624 CCGG C^CGG
2761 CCGG
KpnI 1864 GGTACC GGTAC^C
3824 GGTACC
MaeII 3211 ACGT A^CGT
4283 ACGT
MspI 2624 CCGG C^CGG
2761 CCGG
NciI 2760 CCCGG CC^[GC]GG
2761 CCGGG
NcoI 1293 CCATGG C^CATGG
4116 CCATGG
NsiI 1377 ATGCAT ATGCA^T
3604 ATGCAT
SapI 24 GAAGAGC GCTCTTC(1/4)
3765 GAAGAGC
SphI 964 GCATGC GCATG^C
4151 GCATGC
SspI 306 AATATT AAT^ATT
4466 AATATT
XcmI 2236 CCAGCCTAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4290 CCATGCTCTCTGTGG
XmnI 2587 GAATGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4517 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI ApaI AspHI AvaII BalI
BanI BanII BbvI BfaI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BspMI BsrGI BsrI BssKI BstF5I
BstNI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I